靶向cyclinE小干扰RNA对肝癌HepG2细胞增殖和凋亡的影响
肝癌;细胞周期蛋白E;RNA干扰,田雪飞,肖竺,郭永良,黄燕,朱才,田雪飞,通讯作者:,EffectsofsmallRNAinterferencetotargetcyclinEgeneonapoptosisandprolife
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田雪飞, 肖竺, 郭永良, 湖南中医药大学中西医结合学院 湖南省长沙市 410208
黄燕, 朱才, 中南大学湘雅医院感染科 湖南省长沙市 410008
田雪飞, 2001年湖南中医药大学中医内科学博士研究生毕业, 2004年中南大学湘雅医院内科学博士后流动站出站, 副教授, 从事肝癌的中医药防治研究.
湖南省自然科学基金资助项目, No. 06JJ4054
湖南省教育厅青年资助项目, No. 05B036
通讯作者: 田雪飞, 410208, 湖南省长沙市含浦科教园区象嘴路, 湖南中医药大学中西医结合学院. windsame@163.com
电话: 0731-8458070 传真: 0731-8458071
收稿日期: 2007-07-20 修回日期: 2007-10-29
Effects of small RNA interference to target cyclinE gene on apoptosis and proliferation of HepG2 hepatocellular carcinoma cells
Xue-Fei Tian, Yan Huang, Cai Zhu, Zhu Xiao, Yong-Liang Guo
Xue-Fei Tian, Zhu Xiao, Yong-Liang Guo, Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China
Yan Huang, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China
Supported by: the Natural Science Foundation of Hunan Province, No. 06JJ4054 and the Youth Project of Hunan Province Office of Education, No. 05B036
Correspondence to: Dr. Xue-Fei Tian, Department of Integrative Medicine, Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China. windsame@163.com
Received: 2007-07-20 Revised: 2007-10-29
Abstract
AIM: To design pSilencer3.1-H1hygro plasmid expressing small interfering RNA (siRNA) that targets the coding region of cyclinE, and to evaluate the inhibitory effect of this siRNA on HepG2 cells after transfection.
METHODS: pSilencer3.1-H1hygro vector of the siRNA based on the coding region of cyclinE was constructed. The plasmid and liposome metafectene were cotransfected into HepG2 cells, which overexpressed cyclinE. Cell proliferation and apoptosis were assessed by MTT assay and flow cytometry, respectively, and expression of cyclinE was detected by RT-PCR and Western blotting.
RESULTS: The plasmid expressing siRNA targeted on the coding region of cyclinE was successfully constructed ......
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