应用基因芯片技术筛选肝硬化相关基因
基因芯片;肝硬化;基因表达谱,黄东凤,黄介飞,张弘,黄晓平,鲍柏军,魏群,华婷琰,黄东凤,通讯作者:,Screeninghepatocirrhosis-relatedgenesbyGeneChip,Dong-FengHuang,Jie-FeiHuang,Ho
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黄东凤, 黄介飞, 张弘, 黄晓平, 鲍柏军, 魏群, 华婷琰, 南通大学附属医院消化内科 江苏省南通市 226001
黄东凤, 硕士研究生, 主要从事消化系肿瘤基础及临床研究.
江苏省卫生厅医学科研资助项目, No. H200330
通讯作者: 黄介飞, 226001, 江苏省南通市西寺路20号, 南通大学附属医院消化内科. jiefeihuang@163.com
电话: 0513-85806629 传真: 0513-85519820
收稿日期: 2007-04-10 修回日期: 2007-10-23
Screening hepatocirrhosis-related genes by GeneChip
Dong-Feng Huang, Jie-Fei Huang, Hong Zhang, Xiao-Ping Huang, Bai-Jun Bao, Qun Wei, Ting-Yan Hua
Dong-Feng Huang, Jie-Fei Huang, Hong Zhang, Xiao-Ping Huang, Bai-Jun Bao, Qun Wei, Ting-Yan Hua, Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China
Supported by: the Health Department of Jiangsu Province, No. H200330
Correspondence to: Dr. Jie-Fei Huang, Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China. jiefeihuang@163.com
Received: 2007-04-10 Revised: 2007-10-23
Abstract
AIM: To ascertain the effectiveness of GeneChip for screening hepatocirrhosis-related genes.
METHODS: Total RNA was isolated from tissues of two cases of hepatic cirrhosis and one normal liver by the TRIzol method, and then purified, reverse-transcribed to cDNA incorporating biotin-labeling probe, hybridized with GeneChip (covering 18 400 transcripts, representing 14 500 distinct genes). Picture signals of fluorescence in gene array were scanned and compared by computer analysis of differential expression of the genes in two tissues.
RESULTS: Same differentially expressed genes were 1424 (9.82%) and same up regulated and down regulated genes were 980 and 444 respectively, in two hepatic cirrhosis and tissue specimens one normal liver tissue specimen. These genes were initially classified according to their function, and further analysis showed they played an important role in the pathogenesis of hepatic cirrhosis ......
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