黄芩甙对肝癌细胞BEL-7402抗黏附作用中细胞凋亡和细胞周期的改变
黄芩甙;肝细胞癌;黏附;凋亡;细胞周期,郭昱,姚金锋,崔东来,郭霞,姚树坤,郭昱,通讯作者:,Effectofbaicalinonadhesion,apoptosisandcellcycleofhepatocellularc
 |
| 第1页 |
参见附件(923KB，5页)。
郭昱, 姚金锋, 崔东来, 河北医科大学第二医院消化内科 河北省石家庄市 050000
郭霞, 姚树坤, 河北医科大学第四医院消化内科 河北省石家庄市 050011
郭昱, 副主任医师, 主要从事肝癌中药治疗的研究.
通讯作者: 郭昱, 050000, 河北省石家庄市, 河北医科大学第二医院. guoyu123123@sina.com
电话: 0311-87222951
收稿日期: 2007-07-30 修回日期: 2007-10-24
Effect of baicalin on adhesion, apoptosis and cell cycle of hepatocellular carcinoma cell line BEL-7402
Yu Guo, Xia Guo, Jin-Feng Yao, Dong-Lai Cui, Shu-Kun Yao
Yu Guo, Jin-Feng Yao, Dong-Lai Cui, Department of Gastroenterology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China
Xia Guo, Shu-Kun Yao, Department of Gastroenterology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China
Correspondence to: Dr. Yu Guo, Department of Gastroenterology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China. guoyu123123@sina.com
Received: 2007-07-30 Revised: 2007-10-24
Abstract
AIM: To investigate the effect of baicalin on adhesion, apoptosis and cell cycle of hepatocellular carcinoma (HCC) cell line BEL-7402, and to discuss the relationship between them.
METHODS: HCC cell line BEL-7402 adhesion assay was conducted in a Boyden chamber. Expression of integrin β1 and E-cd proteins of cell line BEL-7402 was determined by flow cytometry (FCM). The effects of baicalin on cell cycle and apoptosis were also observed by FCM. Changes in morphology and ultrastructure were observed by inverted microscopy and electron microscopy.
RESULTS: The adhesion capacity was 52.38 ± 3.83, 35.63 ± 2.32 and 26.58 ± 1.13 at 5, 10 and 20 mg/L baicalin, respectively, and was lower than that in the control group (84.32 ± 6.74; P < 0.05). In the treated group, the HCC cells became smaller and remained round with relatively fewer pseudopods, and microvilli were fewer and shorter. After treatment with 5, 10 and 20 mg/L baicalin, expression of integrin β1 was decreased (354 ......
您现在查看是摘要介绍页，详见PDF附件(923KB，5页)。