RNAi沉默Polo-like kinase-1基因表达对胰腺癌细胞增殖的影响
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张尤历, 范 钰, 吴 莺, 张宇川, 张 炜
胰腺肿瘤;PLK1;RNA干扰;小干扰RNA;增殖;凋亡;实时定量PCR;免疫印迹,RNAi沉默Polo-likekinase-1基因表达对胰腺癌细胞增殖的影响,张尤历,范钰,吴莺,张宇川,张炜,张尤历,通讯作者:,Effectsofpolo-likekinase-1genesilencebyR
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张尤历, 范钰, 吴莺, 张宇川, 张炜, 江苏大学附属医院消化科 江苏省镇江市 212001
张尤历, 医学博士, 主任医师, 副教授, 主要从事消化系统疾病基础与临床研究.
通讯作者: 范钰, 212001, 江苏省镇江市, 江苏大学附属医院消化科. yufanzh99@sina.com
电话: 0511-85026370 传真: 0511-85026387
收稿日期: 2007-07-14 修回日期: 2007-11-02
Effects of polo-like kinase-1 gene silence by RNA interference on proliferation of human pancreatic cancer cells
You-Li Zhang, Yu Fan, Ying Wu, Yu-Chuan Zhang, Wei Zhang
You-Li Zhang, Yu Fan, Ying Wu, Yu-Chuan Zhang, Wei Zhang, Department of Gastroenterology, the Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
Correspondence to: Yu Fan, Department of Gastroenterology, the Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China. yufanzh99@sina.com
Received: 2007-07-14 Revised: 2007-11-02
Abstract
AIM: To investigate the role of polo-like kinase-1 (PLK1) in human pancreatic cancer cells.
METHODS: After MiaPaCa-2 pancreatic cancer cells were transfected with small interfering RNA (siRNA) against PLK1, real-time RT-PCR and Western blotting were used to examine PLK1 gene expression in all cancer cells. The proliferation and growth of cancer cells in vivo and in vitro were studied. Apoptosis of cancer cells was evaluated by terminal uridine deoxynucleotidyl nick end labeling (TUNEL) and agarose gel electrophoresis, respectively.
RESULTS: Expression of PLK1 in MiaPaCa-2 cancer cells transfected with siRNA was down-regulated significantly. Transfection of PLK1 siRNA resulted in significant inhibition of pancreatic cancer cells in vivo and in vitro. The results from TUNEL and DNA ladder analysis showed cancer cells exhibited marked apoptosis, in a time- and dose-dependent manner (r = 0.875, P < 0.05).
CONCLUSION: RNA interference PLK1 can inhibit proliferation through inducing apoptosis of human pancreatic cancer cells.
Key Words: Pancreatic carcinoma; Polo-like kinase 1; RNA interference; Small interfering RNA; Cell proliferation; Apoptosis; Real time-reverse transcription-polymerase chain reaction; Western blotting ......
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