肝素对大鼠肝星状细胞中转化生长因子β1和Ⅰ型胶原的影响
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陈益平, 卢朝升, 汪洪姣, 徐志伟, 陈均亚, 石海矾, 狄军波
肝素;肝星状细胞;转化生长因子β1;Ⅰ型胶原;酶联免疫吸附测定,陈益平,卢朝升,汪洪姣,徐志伟,陈均亚,石海矾,狄军波,通讯作者:,EffectsofheparinontheexpressionoftypeIcollagenandtransf
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陈益平, 卢朝升, 汪洪姣, 徐志伟, 陈均亚,石海矾, 狄军波, 温州医学院附属育英儿童医院感染科 浙江省温州市 325027
温州市科技局基金资助项目, No. Y2004A071
通讯作者: 陈益平, 325027, 温州市学院西路183号, 温州医学院附属育英儿童医院感染科. cccyp@163.com
电话: 0577-88816153
收稿日期: 2007-07-24 修回日期: 2007-11-07
Effects of heparin on the expression of type I collagen and transforming growth factor β1 in rat hepatic stellate cells
Yi-Ping Chen, Chao-Sheng Lu, Hong-Jiao Wang, Zhi-Wei Xu, Jun-Ya Chen, Hai-Fan Shi, Jun-Bo Di
Yi-Ping Chen, Chao-Sheng Lu, Hong-Jiao Wang, Zhi-Wei Xu, Jun-Ya Chen, Hai-Fan Shi, Jun-Bo Di, Department of Pediatrics Infectious Diseases, Yuying Affiliated Children’s Hospital of Wenzhou Medical College, Wenzhou, 325027, Zhejiang Province, China
Supported by: the Science and Technology Project of Wenzhou City, No. Y2004A071
Correspondence to: Yi-Ping Chen, Department of Pediatrics Infectious Diseases, Yuying Affiliated Children’s Hospital of Wenzhou Medical College, 183 Xueyuan West Road, Wenzhou 325027, Zhejiang Province, China. cccyp@163.com
Received: 2007-07-24 Revised: 2007-11-07
Abstract
AIM: To explore the effects of heparin on the expression of transforming growth factor β1 and typeⅠcollagen in rat hepatic stellate cells (HSCs).
METHODS: HSCs were seeded at a density of 1 × 108 cells/L per well in 96-well plates, and grouped as follows: heparin groupⅠ(10 mg/L), group Ⅱ (100 mg/L), group Ⅲ (1000 mg/L), and NS control group. The incubation liquid was extracted after cells were cultured for 48 hours, and frozen until later use. Type Ⅰcollagen and transforming growth factor β1 (TGF-β1) were detected by enzyme-linked immunosorbent assay (ELISA), and HSC proliferation was measured by MTT assay.
RESULTS: The mean levels of TGF-β1 in the heparin Ⅱ and Ⅲ groups were significantly lower than those in the control group (4.59 ± 1.27 ng/L, 3.34 ± 1.13 ng/L vs 5.95 ± 1.72 ng/L, P < 0.01). The mean levels of typeⅠcollagen in groupsⅠ, Ⅱ and Ⅲ were significantly lower than those in the control group (87 ......
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