RNA干扰阻断cyclin D1表达抑制结肠癌细胞增殖
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丁 健, 李 丹, 王承党, 吴 婷, 王小众, 于皆平
小干扰RNA;结肠癌;cyclinD1;细胞周期;细胞增殖;MTT比色法;流式细胞技术,RNA干扰阻断cyclinD1表达抑制结肠癌细胞增殖,丁健,王承党,吴婷,李丹,王小众,于皆平,通讯作者:,RNAinterferenceinhibitstheproliferationofhumancol
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丁健, 王承党, 吴婷, 福建医科大学附属第一医院消化内科 福建省福州市 350002
李丹, 王小众, 福建医科大学附属协和医院消化内科 福建省福州市 350001
于皆平, 武汉大学人民医院消化内科 湖北省武汉市 430060
丁健, 2005年武汉大学医学院医学博士研究生毕业, 主治医师, 主要从事消化道肿瘤的基因治疗研究.
福建省科技厅青年人才创新基金赞助项目, No. 2006F3035
福建省卫生厅青年基金赞助项, No. 2006-1-10
通讯作者: 丁健, 350002, 福建省福州市茶中路,福建医科大学附属第一医院消化内科. docdingjian@yahoo.com.cn
电话: 0591-88808826
收稿日期: 2007-08-23 修回日期: 2007-11-28
RNA interference inhibits the proliferation of human colorectal cancer cells by blocking the expression of cyclin D1
Jian Ding, Dan Li, Cheng-Dang Wang, Ting Wu, Xiao-Zhong Wang, Jie-Ping Yu
Jian Ding, Cheng-Dang Wang, Ting Wu, Digestive Department, First Affiliated Hospital of Fujian Medical University, Fuzhou 350002, Fujian Province, China
Dan Li, Xiao-Zhong Wang, Digestive Department, Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China
Jie-Ping Yu, Digestive Department, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Supported by: Youth Creation Foundation of Sci-Tech Bureau of Fujian Province, No. 2006F3035; Youth Scientific Foundation of Health Bureau of Fujian Province, No. 2006-1-10
Correspondence to: Jian Ding, Digestive Department, First Affiliated Hospital of Fujian Medical University, Chazhong Road, Fuzhou 350002, Fujian Province, China. docdingjian@yahoo.com.cn
Received: 2007-08-23 Revised: 2007-11-28
Abstract
AIM: To inhibit the expression of cyclin D1 in a human colorectal cancer cell line using RNA interference, and to examine its effect on the growth and proliferation of colorectal cancer cells.
METHODS: Human colorectal cancer HT-29 cells were divided into three groups: antisense group transfected with antisense siRNA against cyclin D1, sense group transfected with sense siRNA against cyclin D1, and control group. The inhibitory effect of antisense siRNA on cyclin D1 protein expression was detected by immunoprecipitation and Western blotting ......
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