幽门螺杆菌OipA、HopX、HopW基因的检测及序列分析
http://www.100md.com
王 华, 邵世和
幽门螺杆菌;外膜蛋白;OipA基因;HopX基因;HopW基因;聚合酶链式反应,王华,邵世和,王华,通讯作者:,DetectionandanalysisofOipA,HopXandHopWofHelicobacterpylori,Hu
 |
| 第1页 |
参见附件(859KB，5页)。
王华, 邵世和, 江苏大学医学技术学院病原生物系 江苏省镇江市 212013
王华, 江苏大学在读博士, 主要从事幽门螺杆菌致病性的研究.
江苏省科技厅资助项目, No. BS2004021
江苏大学高级人才资助项目, No. JDG2004008
通讯作者: 王华, 212013, 江苏省镇江市, 江苏大学医学技术学院病原生物系. wangjiahan1979@163.com
收稿日期: 2007-05-11 修回日期: 2007-11-02
Detection and analysis of OipA, HopX and HopW of Helicobacter pylori
Hua Wang, Shi-He Shao
Hua Wang, Shi-He Shao, Department of Medical Microbiology and Parasitology, Medical Technology College, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China
Supported by: the Research Program of Jiangsu Provincial Technology Commission, No. BS2004021 and the Advanced Talent Research Program of Jiangsu University, No. JDG2004008
Correspondence to: Hua Wang, Department of Medical Microbiology and Parasitology, Medical Technology College, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China. wangjiahan1979@163.com
Received: 2007-05-11 Revised: 2007-11-02
Abstract
AIM: To detect three genes of Helicobacter pylori (H pylori) outer membrane proteins in NCTC11637 and clinical strains, and to make a homologous comparison of the nucleotides in strains 26695 and J99.
METHODS: The three genes were detected with PCR in H pylori strains NCTC11637 and 27. The PCR products were sent for nucleotide sequence analysis, and compared with those in strains 26695 and J99 using GenBank.
RESULTS: The sequences of the genes of interest were obtained in strain NCTC11637. Among 27 clinical samples, OipA and HopX genes were detected in 10 and 23 H pylori strains, respectively. Only the HopW gene was obtained in 27
H pylori strains. Moreover, in NCTC11637, the homology of the OipA gene between NCTC11637 and 26695, and J99 in nucleotide acid was 95.38% and 94.60%, respectively, which was lower than the homology between 26695 and J99, at 95.50%. The homology of the HopX gene between NCTC11637 and 26695, and J99 in nucleotide acid was 94 ......
您现在查看是摘要介绍页，详见PDF附件(859KB，5页)。