脂肪肝细胞模型的建立及其生物学特性
http://www.100md.com
王炳芳, 朱韶杰, 田培营
非酒精性脂肪肝;非酒精性脂肪性肝炎;细胞模型;脂肪乳剂,王炳芳,朱韶杰,田培营,王炳芳,通讯作者:,Establishmentofacellularmodelofnon-alcoholicfattyliveranditsbiologicalcharacteri
 |
| 第1页 |
参见附件(854KB，4页)。
王炳芳, 朱韶杰, 田培营, 江苏大学附属昆山市第一人民医院消化科 江苏省昆山市 215300
王炳芳, 1995年上海第二医科大学博士毕业, 主任医师, 教授, 主要从事消化系统疾病的临床及实验研究.
上海市自然科学基金资助项目, No. 02ZB14106
通讯作者: 王炳芳, 215300, 江苏省昆山市前进路91号, 江苏大学附属昆山市第一人民医院消化科. bingfang@public4.sta.net.cn
电话: 0512-50180526
收稿日期: 2007-07-25 修回日期: 2007-11-05
Establishment of a cellular model of non-alcoholic fatty liver and its biological characteristics
Bing-Fang Wang, Shao-Jie Zhu, Pei-Ying Tian
Bing-Fang Wang, Shao-Jie Zhu, Pei-Ying Tian, Department of Gastroenterology, Kunshan First People’s Hospital Affiliated to Jiangsu University, Kunsan 215300, Jiangsu Province, China
Supported by: the Natural Science Foundation of Shanghai, No. 02ZB14106
Correspondence to: Bing-Fang Wang, Department of Gastroenterology, Kunshan First People’s Hospital Affiliated to Jiangsu University, 91 Qianjin Road, Kunshan 215300, Jiangsu Province, China. bingfang@public4.sta.net.cn
Received: 2007-07-25 Revised: 2007-11-05
Abstract
AIM: To establish non-alcoholic fatty liver and non-alcoholic steatohepatitis models at a cellular level.
METHODS: Liver cells HL-7702 were cultured with RPMI-1640 medium containing long chain fat emulsion, which is a nutritional emulsion used in the clinic. Cells were subcultured for two generations to obtain non-alcoholic steatohepatitis models. The similarities between the cellular and animal models were evaluated, especially the utility of the cellular model.
RESULTS: After 48 hours in culture, the TG content in HL-7702 cells cultured in RPMI-1640 medium containing long chain fat emulsion increased remarkably, while there were few variations in biochemical indices such as ALT. When these cells were subcultured for two generations, they showed inflammatory injury.
CONCLUSION: Cellular models of non-alcoholic fatty liver and non-alcoholic steatohepatitis in vivo can be generated in a short time by mixing medium with long chain fat emulsion ......
您现在查看是摘要介绍页，详见PDF附件(854KB，4页)。