RNA干扰survivin基因靶向治疗大肠癌
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张志宏, 韩盛玺, 邱春华, 强 欧, 陈和平, 张初民, 李 易
生存素;PTEN;RNA干扰;凋亡;流式细胞仪,张志宏,韩盛玺,邱春华,陈和平,张初民,李易,强欧,张志宏,通讯作者:,Effectsandmechanismsofsurvivin-siRNAinterferenceoncolonadenocar
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张志宏, 韩盛玺, 邱春华, 陈和平, 张初民, 李易, 四川省医学科学院 四川省人民医院消化内科 四川省成都市 610041
强欧, 四川大学华西医院人类多肽研究实验室 四川省成都市 610041
张志宏, 四川省人民医院消化内科主治医生, 主要从事大肠肿瘤的防治方面的研究工作.
通讯作者: 张志宏, 610041, 四川省成都市, 四川省人民医院消化内科. zhang-821@21cn.com
电话: 028-85081923
收稿日期: 2007-05-15 修回日期: 2007-10-23
Effects and mechanisms of survivin-siRNA interference on colon adenocarcinoma cells SW620
Zhi-Hong Zhang, Sheng-Xi Han, Chun-Hua Qiu, Ou Qiang, He-Ping Chen, Chu-Min Zhang, Yi Li
Zhi-Hong Zhang, Sheng-Xi Han, Chun-Hua Qiu, He-Ping Chen, Chu-Min Zhang, Yi Li, Department of Digestive, Academy of Medical Sciences of Sichuan Province, Chengdu 610041, Sichuan Province, China
Ou Qiang, Department of Human Peptide Research, People Hospital of Sichuan Province, Chengdu 610041, Sichuan Province, China
Correspondence to: Zhi-Hong Zhang, Department of Digestive, Academy of Medical Sciences of Sichuan Province, Chengdu 610041, Sichuan Province, China. zhang-821@21cn.com
Received: 2007-05-15 Revised: 2007-10-23
Abstract
AIM: To investigate the effects and mecha-nisms of survivin-siRNA inteference on adenocarcinoma cells SW620.
METHODS: SW620 cells were transfected with survivin-siRNA using Lipofectamine 2000. The mRNA expression levels of survivin and PTEN were detected by RT-PCR and protein expression levels were detected by Western blotting. MTT and flow cytometry were used to analyze proliferation and apoptosis.
RESULTS: Compared with control cells, the mRNA and protein levels of survivin were reduced in siRNA-transfected cells, while PTEN was increased. At 12, 24, 48 hours, the expression of survivin mRNA was downregulated 75%, 93.75% and 97.8%, respectivly, compared with that in the controls, and the expression of PTEN mRNA was upregulated 41%, 100%, 128%. The growth of transfected cells was inhibited, while apopotosis was increased.
CONCLUSION: Survivin-siRNA has preferential effects on adenocarcinoma cells ......
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