降脂益肝冲剂对NASH大鼠肝脏抵抗素mRNA表达的影响
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杨文慧, 赵和平, 段晓燕, 刘 婷, 葛姝囡
非酒精性脂肪性肝炎;降脂益肝冲剂;抵抗素;大鼠;逆转录聚合酶链反应,杨文慧,段晓燕,刘婷,葛姝囡,赵和平,杨文慧,通讯作者:,EffectofJiangzhiYiganChongjionresistinmRNAintheliverofr
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杨文慧, 段晓燕, 刘婷, 葛姝囡, 山西医科大学 山西省太原市 030001
赵和平, 山西医科大学第一临床附属医院综合科 山西省太原市 030001
杨文慧, 山西医科大学硕士研究生, 主要从事脂肪肝临床及实验研究.
通讯作者: 赵和平, 030001, 山西省太原市, 山西医科大学第一临床附属医院综合科. zhpyx168@126.com
电话: 0351-4639468
收稿日期: 2007-09-12 修回日期: 2007-11-28
Effect of Jiangzhi Yigan Chongji on resistin mRNA in the liver of rats with non-alcoholic steatohepatitis
Wen-Hui Yang, He-Ping Zhao, Xiao-Yan Duan, Ting Liu, Shu-Nan Ge
Wen-Hui Yang, Xiao-Yan Duan, Ting Liu, Shu-Nan Ge, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
He-Ping Zhao, General Department, the First Clinical College, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
Correspondence to: Professor He-Ping Zhao, General Department, the First Clinical College, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China. zhpyx@126.com
Received: 2007-09-12 Revised: 2007-11-28
Abstract
AIM: To explore the effects of resistin in the pathogenesis of a rat model of non-alcoholic steatohepatitis (NASH), and to observe the effect of Jiangzhi Yigan Chongji on resistin mRNA in the liver of that model, to explore its mechanism of action.
METHODS: 24 male Wistar rats were randomly divided into three groups (eight rats in each): normal group fed with standard diet, model group, and treated group fed with a high-fat diet. The treated group was given Jiangzhi Yigan Chongji (1 mL/100 g, its concentration is 0.8 g/mL, twice daily) by intragastric administration after 12 weeks. Meanwhile, the normal and model groups were separately given drinking water by the same means. After 17 weeks, they were killed. Resistin mRNA in the liver of the rat model of NASH induced by high-fat diet was detected with RT-PCR. Level of alanine aminotransferase (ALT) and asparate aminotransferase (AST) was detected by an automatic biochemistry analyzer; fasting blood glucose (FBG) was detected by glucose oxidase method; and tumor necrosis factor a (TNF-a) and fasting insulin (FINS) in serum were detected by radioimmunoassay ......
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