脂联素随原代HSC活化表达的动态变化及其对原代HSC表达MMP-13的影响
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于 丹, 马 红
肝星状细胞;基质金属蛋白酶-13;肝纤维化;实时荧光聚合酶链反应;酶联免疫吸附反应,于丹,马红,于丹,通讯作者:,Expressionofadiponectinduringhepaticstellatecell(HSC
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于丹, 马红, 首都医科大学附属北京友谊医院肝病中心 北京市 100050
于丹, 首都医科大学研究生, 从事肝纤维化发生机制的研究.
通讯作者: 马红, 100050, 北京市, 首都医科大学附属北京友谊医院肝病中心. mahongmd@yahoo.com.cn
电话: 010-63138727
收稿日期: 2007-07-24 修回日期: 2007-11-28
Expression of adiponectin during hepatic stellate cell (HSC) activation and the effect of adiponectin on expression of matrix metalloproteinase-13 in primary HSCs
Dan Yu, Hong Ma
Dan Yu, Hong Ma, Liver Research Center of Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
Correspondence to: Ma Hong, Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China. mahongmd@yahoo.com.cn
Received: 2007-07-24 Revised: 2007-11-28
Abstract
AIM: To study trends in the expression of adiponectin during hepatic stellate cell (HSC) activation and the effects of adiponectin on mRNA and protein expression of matrix metalloproteinase-13 (MMP-13), which was closely related to liver fibrosis in primary HSCs.
METHODS: We isolated primary HSCs through improved two-step liver in situ perfusion, and investigated changes in expression of adiponectin during activation of primary HSCs. MTT was used to study the effect of adiponectin on proliferation of activated HSCs. We chose proper concentration of adiponectin and then divided primary HSCs into five groups according to the concentration of adiponectin. Real-time PCR was used to detect the difference of a-smooth muscle actin, adiponectin and MMP-13 expression between the groups. Enzyme linked immunosorbent assay (ELISA) was used to detect the quantity of MMP-13 protein secreted into the medium.
RESULTS: The survival rate of HSCs showed a linear correlation with adiponectin concentration (OR = 0.828). Expression of adiponectin was down-regulated during HSC activation. Adiponectin was able to inhibit the proliferation of HSCs. When the concentration of adiponectin was 500 mg/L, MMP-13 mRNA expression, in contrast with that in the control group, increased by 5 ......
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