作者：
单位：Li Xiangdong(李向东), Hui Guozhen(惠国桢), Lu Daru(卢大儒), Wang Qi(王 琪), Xu Lu(徐 露), Qiu Xingfang(邱信芳), Xue Jinlun(薛京伦)(Department of Neurosurgery,The First Hospital Affiliated to Suzhou Medical College,Suzhou,215006)
关键词：
苏州医学院学报000702 Abstract Objective To investigate the effects of combination adenovirus-mediated HSV-tk/GCV system and antisense IGF-1 gene therapy for rat glioma and analyze the mechanism.Methods Using the recombinant adenovirus vector,GCV killing effeciency after combined gene transfer of HSV-tk and antisense IGF-1 was observed in vitro.Rat glioma was treated with HSV-tk/GCV and antisense IGF-1 and the survival rate of rats was observed.Results C6 cells transfected with tk and antisense IGF-1 gene were more sensitive to GCV than that transfected with tk gene alone.The survival of the combination gene therapy group was prolonged significantly and large amounts of CD+4,CD+8 lymphocytes were detected in the tumor tissues.Conclusion Antisense IGF-1 gene may enhance the tumor-killing effects of HSV-tk/GCV. ......
单位：Li Xiangdong(李向东), Hui Guozhen(惠国桢), Lu Daru(卢大儒), Wang Qi(王 琪), Xu Lu(徐 露), Qiu Xingfang(邱信芳), Xue Jinlun(薛京伦)(Department of Neurosurgery,The First Hospital Affiliated to Suzhou Medical College,Suzhou,215006)
关键词：
苏州医学院学报000702 Abstract Objective To investigate the effects of combination adenovirus-mediated HSV-tk/GCV system and antisense IGF-1 gene therapy for rat glioma and analyze the mechanism.Methods Using the recombinant adenovirus vector,GCV killing effeciency after combined gene transfer of HSV-tk and antisense IGF-1 was observed in vitro.Rat glioma was treated with HSV-tk/GCV and antisense IGF-1 and the survival rate of rats was observed.Results C6 cells transfected with tk and antisense IGF-1 gene were more sensitive to GCV than that transfected with tk gene alone.The survival of the combination gene therapy group was prolonged significantly and large amounts of CD+4,CD+8 lymphocytes were detected in the tumor tissues.Conclusion Antisense IGF-1 gene may enhance the tumor-killing effects of HSV-tk/GCV. ......