卵巢癌患者外周血及腹水癌细胞多耐药基因及凋亡因子检测
作者：孙晓明 尹格平 陈诵芬#1\$'7, 百拇医药
单位：孙晓明(济南军区总医院流式细胞室，山东 济南 250031)；尹格平 陈诵芬(济南军区总医院妇产科，山东 济南 250031)#1\$'7, 百拇医药
关键词：卵巢癌；多耐药基因；凋亡因子；Fas；Apo2.7化疗#1\$'7, 百拇医药
免疫学杂志010115 摘 要：目的 探讨卵巢癌细胞多耐药基因表达和化疗药物促使卵巢癌细胞凋亡的作用。方法 从62例首次手术及17例复发卵巢癌患者腹水中提取富含癌细胞的悬液，应用3种荧光单克隆抗体P170、CD95及Apo2.7分别标记，经流式细胞仪检测。结果 ①化疗后癌细胞CD95及Apo2.7含量均高于化疗前(P<0.05)；②腹腔化疗前后P170含量无明显差异；③复发组癌细胞P170含量显著高于初发手术组(P<0.05)，且本组化疗后癌细胞CD95和Apo2.7含量明显低于初发手术组(P<0.05)；④化疗可使外周血淋巴细胞CD95含量显著增加(P<0.05)。结论 腹腔灌注化疗药物对卵巢腹水癌细胞凋亡有明显的促进作用，但癌细胞多耐药基因的表达可显著减少癌细胞凋亡。#1\$'7, 百拇医药
分类号：R73 文献标识码：A#1\$'7, 百拇医药
文章编号：1000-8861(2001)01-0053-03#1\$'7, 百拇医药
Study of cellular multidrug resistant gene and apoptosis factor in peripheral blood and ovarian carcionoma cells in abdominal cavity infusion chemotherapy#1\$'7, 百拇医药
SUN Xiao-ming(Department of Flow Cytometry，General Hospital of Jinan Military Region，Jinan 250031，China)#1\$'7, 百拇医药
YIN Ge-ping(Department of Gynecology and Obestetric，General Hospital of Jinan Military Region，Jinan 250031，China)#1\$'7, 百拇医药
CHEN Song-fen(Department of Gynecology and Obestetric，General Hospital of Jinan Military Region，Jinan 250031，China)
Abstract：Objective To explore the multidrug resistant gene (Mdrl) expreession(P170) and cellular apoptosis of ovarian carcinoma in abdominal cavity infusion chemotherapy.Methods The samples of ascites cancer cells(ACC) were selectively collected in sixty-two primary and seventeen recurred ovarian carcinomas，immunologically marked by three kinds of fluorescent monoantibodies-P170(p-gp)，CD95(Fas-Apo-1)，Apo2.7，then determined by flow cytometry.Results ①The CD95 and Apo2.7 contents of ACC after chemotherapy were significantly higher than that before treatment(P<0.05)；②The contents of P170 after chemotherapy were similar to that before treatment (P<0.05)；③The P170 contents in recurred group were significantly higher than that found in the primary cancer group(P<0.05)，and the contents of CD95 and Apo2.7 in the recurred group after chemotherapy were lower than that in the primary group.④The CD95 contents of peripheral lymphocyte were also higher after abdominal cavity infusion chemotherapy(P<0.05).Conclusions The abdominal cavity infusion chemotherapy could obviously improve the apoptostic rate of ACC in ovarian carcinoma；but the apoptostic rate may decrease when the content of P170 becomes elevated.
Keywords：ovary neoplasma；multidrug resistant gene(Mdrl)；apoptosis factor；Fas；Apo2.7；chemotherapyrw1|)#, 百拇医药
作者简介：孙晓明(1958-)，女，山东济南市人，主管技师，本科，主要从事流式细胞仪的临床免疫工作。Tel：(0531)2187681rw1|)#, 百拇医药
参考文献：rw1|)#, 百拇医药
[1]Arao S，Suwa H，Mandai M.et al.Expression of multidrug resistance gene and localization of P-glycoprotein in human primary ovarian cancer[J].Cancer Res.1994，54(5)，1 355-1 358.rw1|)#, 百拇医药
[2]梁正东，卞度宏.卵巢癌细胞对顺铂耐药及其逆转的实验研究[J].中华妇产科杂志，1996，31(2)：75-78.rw1|)#, 百拇医药
[3]Zhang C，Ao Z，Seth A，et al.A mitochondrial membrane protein defined by a novel monoclonal antibaody is preferentially detected in apoptosis cells[J].J Immunol，1996，157(9)：3 980-3 987.rw1|)#, 百拇医药
[4]尹格平，孙晓明，陈诵芬，等.卵巢肿瘤粘附分子吞噬细胞糖蛋白的测定[J].中华妇产科杂志，1997，32(6)：358-361.rw1|)#, 百拇医药
[5]孙晓明，尹格平.实体瘤组织粘附分子流式细胞研究方法的建立[J].中华医学检验杂志，1997，20(1)：9-11.rw1|)#, 百拇医药
[6]Kerr JF，Winterford CM，Harmon BV.Apoptosis：Its significance in cancer and cancer therapy[J].Cancer，1994，73(8)：2 013-2 026.rw1|)#, 百拇医药
[7]Nooter K，Herweijer H.Multidrug resistance(Mdr) genes in human cancer[J].Br J Cancer，1991，63(5)：661-667.rw1|)#, 百拇医药
[8]Nagata S，Golstein P.The fas ceath factor[J].Science，1995，267(5 203)：1 449-1 456.rw1|)#, 百拇医药
[9]Ormerod MG，O′Neill CF，Roberson D，et al.Cisplatin induces apoptosis in human ovarian carcinoma cell line without concomitant internucleosomal degradion of DNA[J].Exper Cell Res，1994，211(2)：231-237.rw1|)#, 百拇医药
[10]Ohmori T，Podack ER，Nishio K，et al.Apoptosis of lung cancer cells caused by some anti-cancer agents(MMC，CPT-11，ADM) is inhibited by bcl-2[J].Biochem Biophys Res Commun，1993，192(1)：30-36.rw1|)#, 百拇医药
收稿日期：1999年10月6日rw1|)#, 百拇医药
修稿日期：2000年10月15日rw1|)#, 百拇医药
出版日期：2001年1月15日(作者：孙晓明 尹格平 陈诵芬)
单位：孙晓明(济南军区总医院流式细胞室，山东 济南 250031)；尹格平 陈诵芬(济南军区总医院妇产科，山东 济南 250031)#1\$'7, 百拇医药
关键词：卵巢癌；多耐药基因；凋亡因子；Fas；Apo2.7化疗#1\$'7, 百拇医药
免疫学杂志010115 摘 要：目的 探讨卵巢癌细胞多耐药基因表达和化疗药物促使卵巢癌细胞凋亡的作用。方法 从62例首次手术及17例复发卵巢癌患者腹水中提取富含癌细胞的悬液，应用3种荧光单克隆抗体P170、CD95及Apo2.7分别标记，经流式细胞仪检测。结果 ①化疗后癌细胞CD95及Apo2.7含量均高于化疗前(P<0.05)；②腹腔化疗前后P170含量无明显差异；③复发组癌细胞P170含量显著高于初发手术组(P<0.05)，且本组化疗后癌细胞CD95和Apo2.7含量明显低于初发手术组(P<0.05)；④化疗可使外周血淋巴细胞CD95含量显著增加(P<0.05)。结论 腹腔灌注化疗药物对卵巢腹水癌细胞凋亡有明显的促进作用，但癌细胞多耐药基因的表达可显著减少癌细胞凋亡。#1\$'7, 百拇医药
分类号：R73 文献标识码：A#1\$'7, 百拇医药
文章编号：1000-8861(2001)01-0053-03#1\$'7, 百拇医药
Study of cellular multidrug resistant gene and apoptosis factor in peripheral blood and ovarian carcionoma cells in abdominal cavity infusion chemotherapy#1\$'7, 百拇医药
SUN Xiao-ming(Department of Flow Cytometry，General Hospital of Jinan Military Region，Jinan 250031，China)#1\$'7, 百拇医药
YIN Ge-ping(Department of Gynecology and Obestetric，General Hospital of Jinan Military Region，Jinan 250031，China)#1\$'7, 百拇医药
CHEN Song-fen(Department of Gynecology and Obestetric，General Hospital of Jinan Military Region，Jinan 250031，China)
Abstract：Objective To explore the multidrug resistant gene (Mdrl) expreession(P170) and cellular apoptosis of ovarian carcinoma in abdominal cavity infusion chemotherapy.Methods The samples of ascites cancer cells(ACC) were selectively collected in sixty-two primary and seventeen recurred ovarian carcinomas，immunologically marked by three kinds of fluorescent monoantibodies-P170(p-gp)，CD95(Fas-Apo-1)，Apo2.7，then determined by flow cytometry.Results ①The CD95 and Apo2.7 contents of ACC after chemotherapy were significantly higher than that before treatment(P<0.05)；②The contents of P170 after chemotherapy were similar to that before treatment (P<0.05)；③The P170 contents in recurred group were significantly higher than that found in the primary cancer group(P<0.05)，and the contents of CD95 and Apo2.7 in the recurred group after chemotherapy were lower than that in the primary group.④The CD95 contents of peripheral lymphocyte were also higher after abdominal cavity infusion chemotherapy(P<0.05).Conclusions The abdominal cavity infusion chemotherapy could obviously improve the apoptostic rate of ACC in ovarian carcinoma；but the apoptostic rate may decrease when the content of P170 becomes elevated.
Keywords：ovary neoplasma；multidrug resistant gene(Mdrl)；apoptosis factor；Fas；Apo2.7；chemotherapyrw1|)#, 百拇医药
作者简介：孙晓明(1958-)，女，山东济南市人，主管技师，本科，主要从事流式细胞仪的临床免疫工作。Tel：(0531)2187681rw1|)#, 百拇医药
参考文献：rw1|)#, 百拇医药
[1]Arao S，Suwa H，Mandai M.et al.Expression of multidrug resistance gene and localization of P-glycoprotein in human primary ovarian cancer[J].Cancer Res.1994，54(5)，1 355-1 358.rw1|)#, 百拇医药
[2]梁正东，卞度宏.卵巢癌细胞对顺铂耐药及其逆转的实验研究[J].中华妇产科杂志，1996，31(2)：75-78.rw1|)#, 百拇医药
[3]Zhang C，Ao Z，Seth A，et al.A mitochondrial membrane protein defined by a novel monoclonal antibaody is preferentially detected in apoptosis cells[J].J Immunol，1996，157(9)：3 980-3 987.rw1|)#, 百拇医药
[4]尹格平，孙晓明，陈诵芬，等.卵巢肿瘤粘附分子吞噬细胞糖蛋白的测定[J].中华妇产科杂志，1997，32(6)：358-361.rw1|)#, 百拇医药
[5]孙晓明，尹格平.实体瘤组织粘附分子流式细胞研究方法的建立[J].中华医学检验杂志，1997，20(1)：9-11.rw1|)#, 百拇医药
[6]Kerr JF，Winterford CM，Harmon BV.Apoptosis：Its significance in cancer and cancer therapy[J].Cancer，1994，73(8)：2 013-2 026.rw1|)#, 百拇医药
[7]Nooter K，Herweijer H.Multidrug resistance(Mdr) genes in human cancer[J].Br J Cancer，1991，63(5)：661-667.rw1|)#, 百拇医药
[8]Nagata S，Golstein P.The fas ceath factor[J].Science，1995，267(5 203)：1 449-1 456.rw1|)#, 百拇医药
[9]Ormerod MG，O′Neill CF，Roberson D，et al.Cisplatin induces apoptosis in human ovarian carcinoma cell line without concomitant internucleosomal degradion of DNA[J].Exper Cell Res，1994，211(2)：231-237.rw1|)#, 百拇医药
[10]Ohmori T，Podack ER，Nishio K，et al.Apoptosis of lung cancer cells caused by some anti-cancer agents(MMC，CPT-11，ADM) is inhibited by bcl-2[J].Biochem Biophys Res Commun，1993，192(1)：30-36.rw1|)#, 百拇医药
收稿日期：1999年10月6日rw1|)#, 百拇医药
修稿日期：2000年10月15日rw1|)#, 百拇医药
出版日期：2001年1月15日(作者：孙晓明 尹格平 陈诵芬)