反相离子对高效液相色谱法测定泰必治注射液A中4种组分的含量
韩澄华 聂小春
摘 要：目的：采用反相离子对高效液相色谱法测定泰必治注射液A中盐酸利多卡因、地塞米松、卡巴芬乙酸(钠盐)与保泰松钠4种组分的含量。方法：采用YWG-C18(10μm,4.5mm×150mm)色谱柱，以乙腈-水(45∶55，含2.5mmol.L-1十六烷基三甲基溴化铵)为流动相，流速1.5mL.min-1，柱温40℃，检测波长230nm。结果：本法可同时测定4种组分的含量。盐酸利多卡因在13～64μg.mL-1、地塞米松在4.5～38μg.mL-1、卡巴芬乙酸(钠盐)在269～1614μg.mL-1、保泰松钠在402～2077μg.mL-1范围内，峰面积与其浓度呈良好的线性关系；平均回收率(n=5)依次为99.9%(RSD=1.2%)，101.0%(RSD=0.4%)，100.5%(RSD=0.2%)，100.6%(RSD=0.8%)。结论：方法简便、快速、准确，可作为样品的检测方法。
关键词：泰必治 盐酸利多卡因 地塞米松 卡巴芬乙酸(钠盐) 保泰松钠 反相离子对高效液相色谱法
, 百拇医药
分类号：R927 文献标识码：A
Determination of Content of Four Components for Trabit Injection A by Reverse-Phase Ion Pair HPLC
HAN Cheng-hua(Xining Institute for Drug Control,810007)
NIE Xiao-chun(Wuhan Institute for Drug Control,430012)
Abstract：Objective：To establish a method using reverse-phase ion pair HPLC,for determining the content of four components—lidocaine hydrochloride,dexamethasone,o-carbamoylphenoxyacetic acid(sodium salt),phenylbutazone sodium of trabit injection A.Method:Used a YWG-C18(4.5 mm×150 mm,10 μm)column,the mobile phase consisted of 2.5 mmol.mL-1 cetyltrimethylammonium bromide-acetonitrile(55∶45),at a flow rate of 1.5 mL.min-1.The column temperature was 40 ℃ and UV detection wavelength was 230 nm.Results:The contents of four components can be simultaneously determined.The calibration curves were linear in the range of 13～64 μg.mL-1 for lidocaine hydrochloride,4.5～38 μg.mL-1 for dexamethason,269～1 614 μg.mL-1 for o-carbamoylphenoxyacetic acid(sodium salt),402～2 077 μg.mL-1 for phenylbutazone sodium respectively.The average recoveries of the four components above respectively were 99.9%(RSD=1.2%)，101.0%(RSD=0.4%),100.5%(RSD=0.2%),100.6%(RSD=0.8%).Conclusion:This method is simple,fast and accurate.
, 百拇医药
Keywords：trabit,lidocaine hydrochloride,dexamethasone,o-carbamoylphenoxyacetic acid(sodium salt),phenylbutazone sodium,reverse-phase ion pair HPLC
作者单位：韩澄华(西宁市药品检验所 810007)
聂小春(武汉市药品检验所 430012)
参考文献：
[1]ZHANG Xin-zhong(张新中).The High Performance Liquid Chromatography Analysis(高效液相色谱分析).Beijing Academic Journal Publishing House(北京学术刊物出版社),1989.129(in Chinese)
收稿日期：2000年12月5日
出版日期：2001年3月31日, 百拇医药
摘 要：目的：采用反相离子对高效液相色谱法测定泰必治注射液A中盐酸利多卡因、地塞米松、卡巴芬乙酸(钠盐)与保泰松钠4种组分的含量。方法：采用YWG-C18(10μm,4.5mm×150mm)色谱柱，以乙腈-水(45∶55，含2.5mmol.L-1十六烷基三甲基溴化铵)为流动相，流速1.5mL.min-1，柱温40℃，检测波长230nm。结果：本法可同时测定4种组分的含量。盐酸利多卡因在13～64μg.mL-1、地塞米松在4.5～38μg.mL-1、卡巴芬乙酸(钠盐)在269～1614μg.mL-1、保泰松钠在402～2077μg.mL-1范围内，峰面积与其浓度呈良好的线性关系；平均回收率(n=5)依次为99.9%(RSD=1.2%)，101.0%(RSD=0.4%)，100.5%(RSD=0.2%)，100.6%(RSD=0.8%)。结论：方法简便、快速、准确，可作为样品的检测方法。
关键词：泰必治 盐酸利多卡因 地塞米松 卡巴芬乙酸(钠盐) 保泰松钠 反相离子对高效液相色谱法
, 百拇医药
分类号：R927 文献标识码：A
Determination of Content of Four Components for Trabit Injection A by Reverse-Phase Ion Pair HPLC
HAN Cheng-hua(Xining Institute for Drug Control,810007)
NIE Xiao-chun(Wuhan Institute for Drug Control,430012)
Abstract：Objective：To establish a method using reverse-phase ion pair HPLC,for determining the content of four components—lidocaine hydrochloride,dexamethasone,o-carbamoylphenoxyacetic acid(sodium salt),phenylbutazone sodium of trabit injection A.Method:Used a YWG-C18(4.5 mm×150 mm,10 μm)column,the mobile phase consisted of 2.5 mmol.mL-1 cetyltrimethylammonium bromide-acetonitrile(55∶45),at a flow rate of 1.5 mL.min-1.The column temperature was 40 ℃ and UV detection wavelength was 230 nm.Results:The contents of four components can be simultaneously determined.The calibration curves were linear in the range of 13～64 μg.mL-1 for lidocaine hydrochloride,4.5～38 μg.mL-1 for dexamethason,269～1 614 μg.mL-1 for o-carbamoylphenoxyacetic acid(sodium salt),402～2 077 μg.mL-1 for phenylbutazone sodium respectively.The average recoveries of the four components above respectively were 99.9%(RSD=1.2%)，101.0%(RSD=0.4%),100.5%(RSD=0.2%),100.6%(RSD=0.8%).Conclusion:This method is simple,fast and accurate.
, 百拇医药
Keywords：trabit,lidocaine hydrochloride,dexamethasone,o-carbamoylphenoxyacetic acid(sodium salt),phenylbutazone sodium,reverse-phase ion pair HPLC
作者单位：韩澄华(西宁市药品检验所 810007)
聂小春(武汉市药品检验所 430012)
参考文献：
[1]ZHANG Xin-zhong(张新中).The High Performance Liquid Chromatography Analysis(高效液相色谱分析).Beijing Academic Journal Publishing House(北京学术刊物出版社),1989.129(in Chinese)
收稿日期：2000年12月5日
出版日期：2001年3月31日, 百拇医药