反相高效液相色谱法测定塞克硝唑及其有关物质
戚燕，单慧军，吴松
(中国医学科学院 中国协和医科大学药物研究所，北京 100050)
[摘要] 目的：采用反相高效液相色谱法测定塞克硝唑的含量及其有关物质。方法：采用Inertsil ODS C18色谱柱(5µm，4.6 mm×150 mm)，以乙腈-水(10：90)为流动相，流速1.5 mL·min-1，检测波长：320nm。结果：反相高效液相色谱法测定的线性范围为0.5 ~700.0µg·mL-1；相关系数r = 0.9999；日内精密度为0.38%(n = 6)；日间精密度为0.44%(n = 6)。结论：采用反相高效液相色谱法测定塞克硝唑的含量及有关物质，方法简便、快速、准确、专属性好。
[关键词] 塞克硝唑；反相高效液相色谱法；含量测定；有关物质
[中图分类号] R978.61；R927.2 [文献标识码] B [文章编号] 1003-3734 (2001)-20010367
RP-HPLC determination of secnidazole and its related substances
QI Yan，SHAN Hui-jun, WU Song*
(Institute of Materia Medica，Peking Union Medical College，Chinese
Academy of Medical Sciences，Beijing 100050，China)
[Abstract] Objective: A RP-HPLC method was established for the determination of secnidazole and its related substances. Method: The separation was performed on a Inertsil C18 column(5µm，4.6mm×150mm).The mobile phase was composed of acetonitrile : water = 10：90.The flow rate was 1.5mL·min-1 .The detection was carried out with a UV detector at 320nm. Results: The calibration curves were linear between 0.5 ~700.0µg·mL-1 (r = 0.9999). Intra-day and inter-day RSD were 0.38% (n = 6)and 0.44%(n = 6). Conclusion: The method is simple，rapid，accurate and is suitable for the determination of secnidazole and its related substances.
[Key words] secnidazole; RP-HPLC; determination; related substance
[作者简介] 戚燕(1973-)，女，大学本科。主要从事于药物分析及制剂研究方面的工作。联系电话：(010)63165223。
[参考文献]
[1] Abdel Fattah M. El Walily, H EBA h. Abdine, Omayma Abdel Razak，et al. Spectrophotometric and HPLC determination of secnidazole in pharmaceutical tablets. [J].J Pharm Biomed Anal, 2000，22：887-897.
[2] Sekhar K. Ravi，M.U.R. Naidu, E.C. Sekhar, et al. Rapid and selective analysis of secnidazole in human plasma using high-performance liquid chromatography with ultraviolet detection.[J].J chromatogr B, 1997，691：208-211., http://www.100md.com
(中国医学科学院 中国协和医科大学药物研究所，北京 100050)
[摘要] 目的：采用反相高效液相色谱法测定塞克硝唑的含量及其有关物质。方法：采用Inertsil ODS C18色谱柱(5µm，4.6 mm×150 mm)，以乙腈-水(10：90)为流动相，流速1.5 mL·min-1，检测波长：320nm。结果：反相高效液相色谱法测定的线性范围为0.5 ~700.0µg·mL-1；相关系数r = 0.9999；日内精密度为0.38%(n = 6)；日间精密度为0.44%(n = 6)。结论：采用反相高效液相色谱法测定塞克硝唑的含量及有关物质，方法简便、快速、准确、专属性好。
[关键词] 塞克硝唑；反相高效液相色谱法；含量测定；有关物质
[中图分类号] R978.61；R927.2 [文献标识码] B [文章编号] 1003-3734 (2001)-20010367
RP-HPLC determination of secnidazole and its related substances
QI Yan，SHAN Hui-jun, WU Song*
(Institute of Materia Medica，Peking Union Medical College，Chinese
Academy of Medical Sciences，Beijing 100050，China)
[Abstract] Objective: A RP-HPLC method was established for the determination of secnidazole and its related substances. Method: The separation was performed on a Inertsil C18 column(5µm，4.6mm×150mm).The mobile phase was composed of acetonitrile : water = 10：90.The flow rate was 1.5mL·min-1 .The detection was carried out with a UV detector at 320nm. Results: The calibration curves were linear between 0.5 ~700.0µg·mL-1 (r = 0.9999). Intra-day and inter-day RSD were 0.38% (n = 6)and 0.44%(n = 6). Conclusion: The method is simple，rapid，accurate and is suitable for the determination of secnidazole and its related substances.
[Key words] secnidazole; RP-HPLC; determination; related substance
[作者简介] 戚燕(1973-)，女，大学本科。主要从事于药物分析及制剂研究方面的工作。联系电话：(010)63165223。
[参考文献]
[1] Abdel Fattah M. El Walily, H EBA h. Abdine, Omayma Abdel Razak，et al. Spectrophotometric and HPLC determination of secnidazole in pharmaceutical tablets. [J].J Pharm Biomed Anal, 2000，22：887-897.
[2] Sekhar K. Ravi，M.U.R. Naidu, E.C. Sekhar, et al. Rapid and selective analysis of secnidazole in human plasma using high-performance liquid chromatography with ultraviolet detection.[J].J chromatogr B, 1997，691：208-211., http://www.100md.com