Hplc法测定天眩清注射液中天麻素的含量
谢笑天,郑 萍,徐树光,陈云建
(1 云南师范大学化学系,昆明 650092;2 昆明制药股份有限公司,昆明 650100)
[摘要]目的:建立HPLC法测定天眩清注射液中天麻素的含量。方法:采用Shimpack CLCCN(150mm×6.0mm,5μm)柱,流动相为乙腈水(10∶90),检测波长270nm。结果:天眩清注射液中天麻素在1~8μg范围内呈良好线性关系,线性关系r=0.9999,平均回收率为99.87%,RSD=0.50%(n=6)。结论:方法准确、重现性好,简便、快速,适用于该产品及原料或其他制剂的质量分析。
[关键词]天眩清注射液;天麻素;高效液相色谱法
[中图分类号]*"R927.11[文献标识码]*"B[文章编号]*"1003-3734(2002)02-0146-03
Determination of gastrodin in Tianxuanqing injection by HPLC
XIE Xiaotian1,ZHENG Ping1, XU Shuguang2,CHEN Yunjian2
(1*"Department of Chemistry,Yunnan Normal University,Kunming 650092, China;
2*"Kunming Pharma Sales Co.,Ltd,Kunming 650100,China)
[Abstract]Objective:To establish a HPLC method for the determination of gastrodin in Tianxuanqing injection and compound products.Methods:Shimpack CLCCN Column(150mm×6.0mm,5μm) was used.The mixture of acetonitrile and water (10∶90) was adopted as the mobile phase with the detection wavelength at 270nm.Results:The calibration curve was in good linearity over the concentration range of 1~8μg(r=0.9999).The average recovery was 99.87%,RSD=0.50%(n=6).Conclusion:The method appeared to be reliable,accurate,reproducible,simple and rapid.
[Key words]Tianxuanqing injection;gastrodin;HPLC
[作者简介]谢笑天(1962-),男,硕士,副教授。主要从事有机分析、植物化学及精细化工的科研和教学工作。联系电话:(0871)5517087,Email:xiext@public.km.yn.cn。
[参考文献]
[1]杨世林,兰进,徐锦堂.天麻的研究进展[J].中草药,1999,31(1)∶66-67.
[2]中华人民共和国卫生部药典委员会.中华人民共和国药典[S].1995年版 二部.北京:化工出版社,广东科技出版社,1995.45.
[3]云南省卫生厅.云南药品标准[S].滇Q/WS 6161980.昆明:云南科技出版社,1982.25-26.
(收稿日期:2001-04-02修回日期:2001-09-10)
, http://www.100md.com
(1 云南师范大学化学系,昆明 650092;2 昆明制药股份有限公司,昆明 650100)
[摘要]目的:建立HPLC法测定天眩清注射液中天麻素的含量。方法:采用Shimpack CLCCN(150mm×6.0mm,5μm)柱,流动相为乙腈水(10∶90),检测波长270nm。结果:天眩清注射液中天麻素在1~8μg范围内呈良好线性关系,线性关系r=0.9999,平均回收率为99.87%,RSD=0.50%(n=6)。结论:方法准确、重现性好,简便、快速,适用于该产品及原料或其他制剂的质量分析。
[关键词]天眩清注射液;天麻素;高效液相色谱法
[中图分类号]*"R927.11[文献标识码]*"B[文章编号]*"1003-3734(2002)02-0146-03
Determination of gastrodin in Tianxuanqing injection by HPLC
XIE Xiaotian1,ZHENG Ping1, XU Shuguang2,CHEN Yunjian2
(1*"Department of Chemistry,Yunnan Normal University,Kunming 650092, China;
2*"Kunming Pharma Sales Co.,Ltd,Kunming 650100,China)
[Abstract]Objective:To establish a HPLC method for the determination of gastrodin in Tianxuanqing injection and compound products.Methods:Shimpack CLCCN Column(150mm×6.0mm,5μm) was used.The mixture of acetonitrile and water (10∶90) was adopted as the mobile phase with the detection wavelength at 270nm.Results:The calibration curve was in good linearity over the concentration range of 1~8μg(r=0.9999).The average recovery was 99.87%,RSD=0.50%(n=6).Conclusion:The method appeared to be reliable,accurate,reproducible,simple and rapid.
[Key words]Tianxuanqing injection;gastrodin;HPLC
[作者简介]谢笑天(1962-),男,硕士,副教授。主要从事有机分析、植物化学及精细化工的科研和教学工作。联系电话:(0871)5517087,Email:xiext@public.km.yn.cn。
[参考文献]
[1]杨世林,兰进,徐锦堂.天麻的研究进展[J].中草药,1999,31(1)∶66-67.
[2]中华人民共和国卫生部药典委员会.中华人民共和国药典[S].1995年版 二部.北京:化工出版社,广东科技出版社,1995.45.
[3]云南省卫生厅.云南药品标准[S].滇Q/WS 6161980.昆明:云南科技出版社,1982.25-26.
(收稿日期:2001-04-02修回日期:2001-09-10)
, http://www.100md.com