the effectiveness between these systems for processing large quantity of cells' purification for clinical use is few, but high purity (more than 90%) CD34+ cells were collected when CliniMACS system that was designed for large quantity of cells was used. Using CliniMACS had no any harm to cells and had nearly 60% of cell recovery rate. Its function is similar to corresponding laboratory type equipment, MiniMACS.
Autologous transplantation needs lower dose of stem/progenitor cells for hematopoietic reconstitution. The hematopoietic function was reconstituted in 3 patients in our study by transplant of 1.4-4.2×105/Kg of purified CD34+ cells. 3.8×106/Kg of CD34+ cells in average are enough for hematopoietic reconstitution in clinic because of the high recovery rate of CliniMACS system. In haploidentical transplantation, basing on strong immunological conditioning regimen, higher dose of stem/progenitor cells, generally 10×106/Kg of CD34+ cells is needed to overcome immune barrier, to engraft and to have hematopietic reconstitution. Such a high dose (per Kg) of stem/progenitor cells can be more easily obtained from small children(3 ......
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