Volume15 Octomber2003 Number10
Chinese Critical Care Medicine, Octomber 2003,Vol.15,No.10
PAGE: 579-581
Dynamic observation IgG and IgM antibodies in Patients with severe acute respiratory syndrome
ZHUANG Jun hua, HUANG Xian zhang, ZHOU Qiang, LIN Lian ying, LIN Lin
Clinical Laboratory, Ersha Island Branch Guangdong Provincal Hospital of Traditional Chinese Medicine, Guangzhou 510105, Guangdong, China
, 百拇医药
OBJECTIVE: To observe IgG and IgM antibodies in Patients with severe acute respiratory syndrome (SARS) and to explore their significance. METHODS: IgG and IgM antibodies were detected in SARS Patients in the first, recovery and follow-up stages, the front healthy doctors and nurses and healthy volunteers with indirect enzyme-linked immunoadsorbent assay. RESULTS: The Positive ratio of IgG was higher than that of IgM in SARS Patients during the first stage. During the recovery, follow-uP stages and in the front healthy crowd, the Positive ratios of IgG were all higher than those of IgM. CONCLUSION: The changeable tendency SARS antibody is more different than that of other infectious diseases. This tendency can be used as a indicator for epidemiological study and serum diagnose.
, 百拇医药
PAGE: 582-584
Analysis and causation discussion of 185 severe acute respiratory syndrome dead cases
LI Chun sheng, PAN Shi fen
Beijing Chaoyang Hospital, Capital University of Medical Science, Beijing 100020, China
OBJECTIVE: To Proceed the retrospective analysis of the dead reasons of Patients with severe acute respiratory syndrome (SARS), in order to Provide experience for decreasing mortality of SARS Patients in clinic. METHODS: Retrospective analyze of 185 dead cases of SARS Patients, including age, basic diseases, laboratory tests, radiological test and therapy. RESULTS: There were 102 men and 83 women in 185 dead cases of SARS, in which average age was 56.4 years, 85 cases were older than 60 years. 147 cases (79.5%) had basic diseases, cardiovascular disease (57 cases) was at the first Place; diabetes mellitus (41 cases) was at the second Place; cerebrovascular disease (19 cases) was at the third Place; 15 Patients had alimentary disease. 52 Patients (28.1%) suffered from complications, which were acute respiratory distress syndrome (ARDS), secondary infection and multiple organ dysfunctional syndrome (MODS) by order. Some of the Patients having complete information showed that life symptoms were stabilization. Hemogram and blood gas analysis showed that blood sugar and white blood cell (WBC) increased (P<0.001); lymphocyte decreased significantly (P<0.005) and PaO2, PaO2/FiO2 decreased Progressively (P<0.005， P<0.001) in late stage of SARS versus just entering the Hospital. Half Patients showed the abnormality of biochemical indexes. CONCLUSION: Age and basic diseases are sensitive indexes for Predicting dead tendency of SARS. Lung injury, obstinate hypoxemia, abnormality metabolize of hyperglycemia and misusing glucocorticoids and antibiotic leading to secondary infection are the main reasons for SARS Patients dying.
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PAGE: 585-588
Evaluation of noninvasive Positive Pressure ventilation in treatment for Patients with severe acute respiratory syndrome
CHEN Hong, WANG Xiang ping, LI Fei, YANG Qiang, ZHANG Lian guo, DU Jian xin, ZHAO Song lin
Department of General Surgery Xuanwu Hospital of the Capital University of Medical Sciences, Beijing 100053, China
ABSTRACT: OBJECTIVE: To analyze the effects of noninvasive Positive Pressure ventilation (NIPPV) on oxygenation of severe acute respiratory syndrome (SARS) Patients, and to discuss the timing Point for mechanical ventilation. METHODS: Twenty-five SARS Patients with respiratory dysfunction treated with NIPPV were studied retrospectively in order to evaluate the influences within 24 hours after initiation of ventilatory support on their Physiological indices and oxygenation. Patients with SARS were divided into two groups: survivor group (n=13) and non-survivor group (n=12). We compared the acute Physiology and chronic health evaluation (APACHEII) score, respiratory rate (RR),saturation of oxygen (SPO2) and modificative respiratory index (MRI) for the survivors and non-survivors before NIPPV and after NIPPV for twenty-four hours, respectively. RESULTS: Although NIPPV administered via full-face masks might be an effective treatment for rapidly improving vital signs and gas exchange and sense of dyspnea in both groups during the initial 24 hours of ventilatory support, the Patients in non-survivor group had higher APACHEII score、 respiratory rates and lower SPO2、 MRI than the Patients in survivor group (P<0.05) at the same intervals after initiation of support. CONCLUSION: Noninvasive ventilation should be used as a substitutive tool for endotracheal intubation an alternative treatment for acute respiratory failure related to SARS. Therefore, we should make efforts to avoid missing the time Point for NIPPV or intubation, and we should not be restricted to the available indications for NIPPV or IPPV.
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PAGE: 588
Aualysis of treatment of 22 cases of SARS Patients with mentyl-Prednisolone
ZHOU Yu ling, ZHOU Xiang jun
The Hangzhou Hospital of Chinese People’s Armed Police Forces, Hangzhou 310051, China
PAGE: 589-592
Expression of genes related to inflammatory response in myocardium of rats after burn injury
, 百拇医药
ZHANG Jia ping, HUANG Yu sheng, YANG Zong cheng
Institute of Burn Research, Southwestern Hospital, the Third Military Medical University, Chongqing 400038, China
ABSTRACT: OBJECTIVE: To observe expression of genes related to inflammatory response in myocardium of rats after burn injury. METHODS: After 40 Percent total body surface area (TBSA) of rat was burned gene expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), induced nitric oxide synthase (iNOS) and cytoplastic Phospholipids A2 (cPLA2) in myocardium was detected with reverse transcription-Polymerase chain reaction (RT-PCR) at different time after burn. Meanwhile, changes of contractile and diastolic ability of left ventricle were determined with Physiological recorder. RESULTS: TNF-α and cPLA2 mRNAs were increased dramatically at 1 hour after burn and maintained at high level up to 24 hours later (P<0.01), IL-1β gene expression was up-regulated at 3 hours after burn (P<0.01) and declined to normal level 12 hours later, herein any significant increase of iNOS mRNA in myocardium in rats was not found except decrease in iNOS mRNA could be detected at 1 hour after burn. As expected, severe impairment of myocardiumwas occurred at 3 hours after burn, represented by declining in both contractile and diasotolic ability of lefe ventrical. CONCLUSION: Over expression of TNF-α, cPLA2 and IL-1β mRNA in myocardium in rats may be Partial cause of local myocardial uncontrolled inflammatory response and then Partially contributed to cardiacimpairment after burn.
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PAGE: 593-595
Heat shock Protein 70 induction by heat stress suppress interleukin-1β and tumor necrosis factor-α levels in the brain tissue in rats with infectious brain edema
MAO An ding, YU Pei lan, YANG Yu jia
Department of Neurology, Jiangxi Children Hospital, Nanchang 330006, Jiangxi, China
ABSTRACT : OBJECTIVE: To investigate the changes of interleukin-1β(IL-1β) and tumor necrosis factor-α (TNF-α) in the brain tissue of rats with infectious brain edema (IBE) and their relationship with heat shock Protein 70 (HSP70) by heat stress response (HSR). METHODS: Seventy-two SD rats were randomly divided into normal controls group (NS group), IBE group, and HSP group, each group was divided into three subgroups. The rats in subgroups were killed at 4 hours, 8 hours and 24 hours after the injections of IBE or normal saline respectively. HSP70 in brain tissues were determined by Western blot analysis. The concentrations of IL-1β and TNF-α in the brain homogenate of rats were determined by enzyme linked immunoadsorbent assay (ELISA). RESULTS: The RESULTS showed that HSP70 in brain tissues were elevated after heat shock. IBE group and NS group at 4 hours, 8 hours, 24 hours were induced to base levels of HSP70. The concentrations of TNF-α were significantly elevated in IBE group than in NS group at the various time Points (P<0.01 or P<0.05), especially at 8 hours. The concentrations of IL-1β were significantly increased in IBE group compared with NS group at 4 hours, 8 hours. HSR reduced the IL-1β and TNF-α concentrations in the brain tissue in compared with IBE group (P<0.05 or P<0.01). CONCLUSION: IL-1β and TNF-α are involved in infectious brain edema by IBE. HSP70 against infectious brain edema in rats may be associated with the reduction of IL-1β and TNF-α in brain tissue.
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PAGE: 596-599
Experimental study on recombinant human Platelet-derived growth factor gel in a diabetic rat model of cutaneous incisal wound healing
SUN Tong zhu, FU Xiao bing, ZHAO Zhi li, CHEN Wei, SUN Xiao qing, ZHOU Gang, SHENG Zhi yong
Research Department of Burn Institute, 304th Hospital of People’s Liberation Army, Beijing 100037, China
ABSTRACT: OBJECTIVE: To investigate the effect of recombinant human Platelet-derived growth factor (rhPDGF-BB) on cutaneous incisal wound healing in diabetic rats and this factor does-effect relationship. METHODS: Thirty Wistar diabetic rats were used in this study. Four full-thickness skin wounds of 2.54 cm2 were incised in the back of each rat. The wounds were divided into five groups: three groups were treated with different dosage of rhPDGF-BB gel (14.0 μg/cm2 wound, 7.0 μg/cm2 wound, 3.5 μg/cm2 wound), other two groups were treated with vehicle or untreated, respectively. rhPDGF-BB gel was topically applied to the wounds from 1 to 14 days. The wound healing effect was assessed by the measurements of the wound area, subcutaneous chamber and histological changes of each group. RESULTS: On 14 days, the wounds of the three groups treated with rhPDGF-BB contracted to (0.22±0.30)cm2, (0.05±0.06)cm2, (0.32±0.32)cm2, respectively. The area of the middle dosage groups was significantly smaller than those treated with vehicle (0.23±0.22)cm2 (P<0.05) and untreated (0.22±0.25)cm2 (P<0.05). On 7 days, the subcutaneous chambers of the three treated groups contracted to (0.04±0.03)ml, (0.02±0.02)ml, (0.06±0.03)ml， and there was a statistic significance when comparing middle dosage group with vehicle group(0.06±0.03)ml (P<0.05) and untreated group(0.07±0.05)ml (P<0.05). As for histological examination, much more granulation formation was shown in the wound bed in middle dosage group than that in other four groups on 7 days, and some of the wounds treated with middle dosage were healing on 14 days. CONCLUSION: It suggested that topical application of rhPDGF-BB might improve wound healing in diabetic rats. The effective dosage of rhPDGF-BB is 7.0 μg/cm2 wound.
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PAGE: 599
Significance of noninvasive hemodynamic monitoring in Patient after
LUO Zhong yuan, WANG Bo
Intensive care unit of 117 military Hospital, hangzhou, zhejiang, 310013
PAGE: 600-602
Characteristics of epidermal growth factor and its receptor expression in dermal chronic ulcers
CHEN Wei, FU Xiao bing, SUN Tong zhu, SUN Xiao qing, SUN Peng, ZHAO Zhi li, SHENG Zhi yong
, 百拇医药
304th Hospital of People’s Liberation Army, Beijing 100037, China
ABSTRACT: OBJECTIVE: To investigate the expression and location of epidermal growth factor (EGF) and its receptor (EGFR) in dermal chronic ulcers and normal skin in order to explore their influence on ulcer formation. METHODS: The expression intensity and distribution of EGF and EGFR were detected with Pathological method and immunohistochemistry method in 8 cases of dermal ulcers, 8 cases of edge of ulcer and 8 cases of normal skin. RESULTS: The Positive signals of EGF could be found in epidermal cells, endothelial cells and some fibroblasts; EGFR was principally located in the cytoplasm and cellular membrane of these cells mentioned above in normal skin. From normal skin, edge of ulcer to ulcerative tissues, the Protein contents of EGF and EGFR were decreased progressively. In ulcerative tissues, EGF was mostly distributed in monocytes and macrophages while EGFR was chiefly sited in monocytes. When compared with normal skins, the Protein expression of EGF and EGFR was notably reduced in ulcerative tissues (both P<0.01). The Positive cellular ratios of two Proteins were reduced to (7.1±5.2)% and (8.8±5.5)% of those in normal skin respectively (all P<0.01). CONCLUSION: The formation of dermal chronic ulcers is closely associated with the reduction of EGF and EGFR Protein expression which may lead to binding obstruction between EGF and its receptor.
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PAGE: 603-605
Effects of serum from normal and burned rats on the biological behavior of bone marrow stromal cells
HAN Bing, FU Xiao bing, CHEN Wei, SUN Tong zhu, ZHOU Gang
Key Laboratory of Wound Repair, 304th Hospital of People’s Liberation Army, Beijing 100037, China
ABSTRACT: OBJECTIVE: To investigate the effect of serum from normal and burned rats on the biological behaviors of bone marrow stromal cells (BMSCs). METHODS: Bone marrow was extracted from sacrificed Wistar rats and BMSCs were then incubated in F-12 medium in the Presence of fetal calf serum (FCS, group F), normal rat serum (group N), serum harvested from burned rats at 3 days (group B1) and 14 days (group B2) after burn injury. The growth curve, cell cycle, apoptosis rate and contents of DNA of BMSCs were investigated. RESULTS: The growth curves of cells incubated in different rat serum were similar, but were significantly different from that of cells incubated in FCS. The doubling time of cells incubated in rat serum from group N, B1 and B2 were 23.7, 18.6 and 20.2 hours, respectively, which were significantly shortened compared with that in group F. Compared with group F, the numbers of cells at Platform Phase in group N, B1 and B2 were greater. The ratios of cells at Phase G0/G1 in group B1 and B2 were significantly lower than those in other groups (all P<0.01), but the ratios of cells at Phase S were adverse (P<0.01). Contents of DNA in cells incubated in rat serum were greater than that in cells incubated in FCS (P<0.05 or P<0.01). Contents of DNA in cells incubated in serum from burned rats were greater than that in cells in normal rat serum (P<0.01). The rate of cell apoptosis in group B1 was higher than those in other groups (P<0.01). CONCLUSION: Rat serum is more effective to stimulate Proliferation of BMSCs from rat in vitro than FCS. There are probably many factors affecting the growth of BMSCs, the contents of which changed with the time after burn injury. The effects of serum from burned rats on the growth of BMSCs are complex.
, 百拇医药
PAGE: 606-608
Isolation and culture of multipotent mesenchymal stem cells from Porcine bone marrow
FANG Li jun, FU Xiao bing, SUN Tong zhu, LI Jian fu, CHENG Biao, WANG Yu xin
Department of Stomatology, the Second Hospital of China Medical University, Shenyang 110003, Liaoning, China.
ABSTRACT : OBJECTIVE: To establish a method for the isolation of Porcine mesenchymal stem cells (MSCs) from bone marrow and to demonstrate their differentiation ex vivo into various mesenchymal tissue cells. METHODS: MSCs were isolated from bone marrow and Purified by centrifuge and in vitro. The Proliferation and growth characteristics were observed in Primary and Passage culture. Cell cycle was analyzed by measuring DNA content with FAC-Scan flow cytometer and cell multipotent was identified with specific staining. RESULTS: The adherent，fibroblast-like cells were confluent in single layer after Plating for 12-14 days. The cultured MSCs in vitro differentiated into osteoblasts. The cell cycle analysis showed that 80% of MSCs were in G0/G1 Phase. CONCLUSION: Porcine MSCs can be isolated from postnatal bone marrow through their adherent ability. Porcine MSCs may be introduced as a valuable model system to study the mesenchymal lineages for basic research and tissue engineering.
, 百拇医药
PAGE: 609-611
Changes and effects of group II metabotropic glutamate receptors in the cerebral cortex of rats after diffuse brain injuries coupled with hyperpiesia secondary brain insults
FEI Zhou, ZHANG Xiang, GONG Fang he, BAI Hong min, WANG Xi ling, LIANG Jing wen
Department of Neurosurgery, Xijing Hospital, the Fourth Military Medical University, Xi'an 710033, Shanxi, China
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ABSTRACT: OBJECTIVE: To study the changes and effects of groupIImetabotropic glutamate receptors(mGluR) after diffuse brain injuries (DBI) coupled with hyperpiesia secondary brain insults(SBI). METHODS: Male SD rats were randomized into four groups: normal control, sham-operated, DBI alone and DBI coupled with SBI group. The SBI model was made on the basis of Marmarou's model. The mRNAs of the mGluRs were detected at 1, 3, 6, 12, 24, 48 and 72 hours after injuries by in-situ hybridization, and the Positive neurons were counted. RESULTS: Statistical analysis showed no significance between normal control group and sham-operated group, DBI group in mGluR 2, 3 mRNA (all P>0.05). The number of mGluR 2, 3 Positive neurons decreased at 12 hours after injury and the Peak occurred at 48 hours after injury in the injured cerebral cortex in DBI alone group (both P<0.05). However, in DBI with SBI group, there was a significant decrease of the number of mGluR2, 3 Positive neurons at 6 hours after injury and the Peak happened at 24 hours after injury (both P<0.05). CONCLUSION: mGluR2, 3, factors with the function of protecting brain, might play an important role in the Path Physiology of DBI and SBI.
, 百拇医药
PAGE: 612-614
Changes in Proinflammatory cytokines and immune function of subeschar tissue fluid during early stage after severe burn
RONG Xin zhou, LI Qing hui, HUANG Xiao hua, BEI Chun hua, LIU Zhi qiang, SU Yuan ming
Department of Burns, First Municipal People’s Hospital, Guangzhou 510180, Guangdong, China
ABSTRACT: OBJECTIVE: To evaluate the inflammatory response and immune function of subeschar tissue fluid (STF) in early stage of severe burn. METHODS: Eight Patients were observed and samples from blood as well as STF were collected at 16, 24, 32, 40, 48 hours following burn to measure white blood cell (WBC) counts, levels of immunoglobin (IgA, IgG, IgM), complement (C3, C4) values as well as proinflammatory cytokines as tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and IL-8. In addition, twenty Balb-c mice were used for the lethal experiment by STF collected at 48 hours after burn. RESULTS: The levels of IgA, IgG, IgM in STF were about half of those in the blood samples (all P<0.01). The WBC counts in STF were 1/100 time of that in the blood (all P<0.01). The contents of TNF-α, IL-6 and IL-8 in STF were comparable with those in the blood (P>0.05). Following STF challenge, none of the mice died during 7-day observation Period. CONCLUSION: The cells and large molecules seems to be more difficult to enter STF compared with small molecules. No marked local inflammatory response occurs in STF during early stage of severe burn, and STF challenge has no lethal effect. The low WBC counts and low immunoglobin as well as complement levels imply the limited effect of STF on local infection, and effective antibiotics should be timely used after major burn.
, 百拇医药
PAGE: 615-617
Dynamic changes of Platelet number in early multiPle injury and it is effect on Prognosis
HAO Jiang, LIE Ming, ZHANG Yin, ZHANG Ping, SUN Chang ju, BIAN Ge yuan, BAI Li
Kunming General Hospital, Chengdu Military Region, Kunming 650032, Yunnan, China
ABSTRACT: OBJECTIVE: To study the dynamic changes of Platelet number in early multiPle injured Patients and its clinical significance. METHODS: Ninety Patients with multiple injuries less than 48 hours were selected. Blood analysis was dynamicly investigated at 1, 24, 72, 120 and 168 hours after in Hospital, and acute Physiology and chronic health evaluationIII (APACHEIII) and injury seriousness scale (ISS) scores were recorded.RESULTS:The Platelet number declined after injury, reached the lowest Point at 72 hours after in Hospital, then restored to the normal level at 120 hours. In the Platelet decrease group, the trauma was more critically [ISS (29.60±9.80) scores vs. (22.30±12.00) scores, (P<0.05)] and the incidence of multiple organs dysfunction syndrome (MODS) was higher than normal group (83.9% vs. 62.7%, (P<0.05). In the death group, the number of Platelet was declined[(30.1±10.3)hours vs. (51.3±14.8) hours, (P<0.05) and the lasting tine was longer [(63.7±11.0) hours vs. (54.2±13.4) hours, (P<0.05). CONCLUSION: The Platelet number declines after multiple injury. The decrease and continuously lower level of Platelet suggest Poor Prognosis.
, 百拇医药
PAGE: 618-621
Change in intestinal free radical and Prostaglandin metabolism in the aged rats with Pneumococcus Pneumonia
LI Jian sheng, LI Su yun, GUO Sheng dian
Geriatrics Department of Henan College of Traditional ChineseMedicine, Zhengzhou 450003, Henan, China.
ABSTRACT: OBJECTIVE: To study the Pathogenic mechanism underlying intestinal injury in the aged rat with Pneumonia. METHODS: The model of rats with Pneumococcus Pneumonia was reproduced, and animals were dividedinto young control group (YCG), young model group (YMG), aged control group (ACG) and aged model group (AMG). The Pathological change of lung tissue and intestine, contents of intestinal 6-keto-Prostaglandin F1α (6-keto-PGF1α), thromboxane B2 (TXB2), nitric oxide (NO), malondialde hyde (MDA) and suPeroxide dismutase (SOD) activity were determined in various groups. RESULTS: The lung and intestinal injury in AMG was more serious than that in the YMG. The decreases in SOD activity and 6-keto-PGF1α content, the increases in contents of TXB2, NO, MDA in the YMG and the AMG were obvious than those in the YCG and the ACG respectively. The decrease in SOD activity and increase in MDA content were obvious in the ACG than those in the YCG. In addition, the decrease in SOD activity and increase in MDA content in the AMG were significant compared with those in the YMG. CONCLUSION: The Prostaglandin metabolism and the free radical injury might be in the Pathogenesis of intestinal injury in the aged rats with Pneumonia. The intestinal injury induced by free radical in aged rats with Pneumonia appears to be more obvious with ageing.
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PAGE: 622-624
Effect of endotoxemia on thyrotropin releasing hormone and its receptor in Wistar rat brain
WANG Xin chun, LIU Bei yan, XU Ping, JING Hui
Emergency Department, Second Hospital Affiliated to Harbin Medical University, Harbin 150086, Heilongjiang, China
ABSTRACT: OBJECTIVE: To study the dose and location of thyrotropin releasing hormone (TRH) and its receptor in Wistar rat's brain when early endotoxemia happened. METHODS: Twenty-two Wistar rats were selected. In 13 mouse the model was established with endotoxemia and hemorrhagic shock. The TRH and its receptor were observed with radioimmunoassay (RIA), and were compared to the 9 control rats. RESULTS: The dose of TRH decreased significantly in the early stage of endotoxemia. The maxium dose (Bmax) of TRH increased while Kd (affinity) decreased, so the effective TRH receptor had no significantly change. CONCLUSION: The dose of TRH is different in brain and serum. The decrease of TRH and the steady of active TRH receptor may protect the brain tissue and repress multiple organ dysfunction syndrome (MODS) in the early stage of endotoxemia.
, 百拇医药
PAGE: 625-627
Change of the erythrocyte chemokine receptor binding activity in scalded rats with Pseudomonas aeruginosa infection
CHEN Xiao dong, WANG Shun bing, WU Bo yu, JIANG Qiong
Provincial Burns Institute, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian, China.
ABSTRACT: OBJECTIVE: To explore the influence of infection on the erythrocyte chemokine receptor (ECKR)binding activity in severely scalded rats. METHODS: The SPrague-Dawley (SD) rats were randomly divided into three group: sham scald group (A), burn and infection group (B) and infection group (C). The B group rats were scalded with 30% total body surface area (TBSA) of III degree, and the rats ECKR binding activity with interleukin-8 (IL-8) as ligand were detected by enzyme-linked immunosorbent assay (ELISA) at 1, 2, 4, 6 and 8 hours after infection. RESULTS: Compared to that in A group, ECKR binding activities declined significantly (both P<0.01) after infection in both of B and C groups, but they increased at 8 hours (P<0.01). ECKR binding activity in B group was significantly less at 2, 8 hours than that in C group after infection (both P<0.05). The declining range of ECKR binding activity was more in B group, in which the decline of ECKR binding activity appeared earlier (2 hours) than that in C (6 hours) group. CONCLUSION: The infection lead to the decline of ECKR binding activity in burned rats, and the erythrocytes might Participate in the chemokine regulation and Play a novel role in the infection.
, 百拇医药
PAGE: 628-630
Effect of hypotonic liquid containing colloid on hemorrhagic dogs with celiac seawater immersion wound
WU Yin ai, WANG Zhi wei, LIU Xian tang, LUO Zhen dong, YU Hong tao, HU Shi xiong, JIN Hao sheng
General Surgery Department, 157th Hospital of People’s Liberation Army, Guangzhou 510510, Guangdong, China
ABSTRACT: OBJECTIVE: To determine hypotonic liquid containing colloid on hemorrhagic dogs with celiac seawater immersion wound. METHODS: Thirty-five dogs were randomly divided into five groups: control group (group A), 0.9% NaCl therapy group (group B) and 0.45% NaCl therapy group (group C), 5% glucose therapy group(group D) and hypotonic liquid containing colloid therapy group (group E). Changes of mean artery Pressure (MAP), cardiac output (CO), urine volume, Plasma osmotic Pressure and Pathologic changes of lung and brain were observed. RESULTS: hypotonic liquid containing colloid could effectively ameliorate MAP and CO, improve urine volume, decrease Plasma osmotic Pressure and Prevent occurrence of lung and brain edema. CONCLUSION: Hypotonic liquid containing colloid can exert satisfactory therapeutic effects on hemorrhagic dogs with celiac seawater immersion wound.
, 百拇医药
PAGE: 631-633
Risk factors for intracerebral hemorrhage after intravenous thrombolysis in acute cerebral infarction
LI Dou, LEI Yan ni
Complex Internal Medicine, Beijing Emergency Central, Beijing 100031, China
ABSTRACT: OBJECTIVE: To identify risk factors of intracerebral hemorrhage (ICH) after intravenous thrombolysis in acute cerebral infarction. METHODS: Eighty-two Patients with acute cerebral infarction were treated with intravenous urokinase within 12 hours from stroke onset. The data of clinic and laboratory were as readily available baseline factors and were analyzed to select Potential contributors to ICH. RESULTS: ICH occurred in 15 Patients (18.3%), including 6 hemorrhagic infarction (HI, 7.3%) and 9 Parenchyma hemorrhage (PH, 11.0%). Seven Patients had symptomatic ICH, and 4 Patients died. Patients with ICH had significantly lower European stroke scale (ESS) scores. Patients with ESS<60 scores, early ischemic changes (EIC) on cranial CT scans or artrial fibrillation had significantly higher risk of ICH. CONCLUSION: Risk factors associated with ICH are severity of neurological deficit, EIC and artrial fibrillation.
, 百拇医药
PAGE: 634-635
Solid stereotaetic installing tube and drainage with CT in treatment of severe hypertensive cerebrall hemorrhage: report of 32 cases
ZHANG Yue ke, HAN Xian qi, QI Yu jie, LIN Tao fen, YANG Xing yi
Jimo People’s Hospital, Jimo 266200, Shandong
PAGE: 635
Treatments of urethra rupture after simple administration in emergency department
, 百拇医药
LIU Zhi liang, LI Zhan qing, ZHANG Zhi guo, LI Ri qing
Datong the fifth hospital, Datong 037006, Shanxi
PAGE: 636-638
Application of genetic engineering on wound repair
LIU Qiang, CHAI Jia ke
Burns Institute, 304th Hospital of PLA
PAGE: 639-640
European Society of cardiology guideline (III): Percutaneous coronary interventions Emergency department
ZHANG Wei, SHEN Hong
The Emergency Department of the General Hospital of People’s Liberation Army, Beijing 100853, China., http://www.100md.com
PAGE: 579-581
Dynamic observation IgG and IgM antibodies in Patients with severe acute respiratory syndrome
ZHUANG Jun hua, HUANG Xian zhang, ZHOU Qiang, LIN Lian ying, LIN Lin
Clinical Laboratory, Ersha Island Branch Guangdong Provincal Hospital of Traditional Chinese Medicine, Guangzhou 510105, Guangdong, China
, 百拇医药
OBJECTIVE: To observe IgG and IgM antibodies in Patients with severe acute respiratory syndrome (SARS) and to explore their significance. METHODS: IgG and IgM antibodies were detected in SARS Patients in the first, recovery and follow-up stages, the front healthy doctors and nurses and healthy volunteers with indirect enzyme-linked immunoadsorbent assay. RESULTS: The Positive ratio of IgG was higher than that of IgM in SARS Patients during the first stage. During the recovery, follow-uP stages and in the front healthy crowd, the Positive ratios of IgG were all higher than those of IgM. CONCLUSION: The changeable tendency SARS antibody is more different than that of other infectious diseases. This tendency can be used as a indicator for epidemiological study and serum diagnose.
, 百拇医药
PAGE: 582-584
Analysis and causation discussion of 185 severe acute respiratory syndrome dead cases
LI Chun sheng, PAN Shi fen
Beijing Chaoyang Hospital, Capital University of Medical Science, Beijing 100020, China
OBJECTIVE: To Proceed the retrospective analysis of the dead reasons of Patients with severe acute respiratory syndrome (SARS), in order to Provide experience for decreasing mortality of SARS Patients in clinic. METHODS: Retrospective analyze of 185 dead cases of SARS Patients, including age, basic diseases, laboratory tests, radiological test and therapy. RESULTS: There were 102 men and 83 women in 185 dead cases of SARS, in which average age was 56.4 years, 85 cases were older than 60 years. 147 cases (79.5%) had basic diseases, cardiovascular disease (57 cases) was at the first Place; diabetes mellitus (41 cases) was at the second Place; cerebrovascular disease (19 cases) was at the third Place; 15 Patients had alimentary disease. 52 Patients (28.1%) suffered from complications, which were acute respiratory distress syndrome (ARDS), secondary infection and multiple organ dysfunctional syndrome (MODS) by order. Some of the Patients having complete information showed that life symptoms were stabilization. Hemogram and blood gas analysis showed that blood sugar and white blood cell (WBC) increased (P<0.001); lymphocyte decreased significantly (P<0.005) and PaO2, PaO2/FiO2 decreased Progressively (P<0.005， P<0.001) in late stage of SARS versus just entering the Hospital. Half Patients showed the abnormality of biochemical indexes. CONCLUSION: Age and basic diseases are sensitive indexes for Predicting dead tendency of SARS. Lung injury, obstinate hypoxemia, abnormality metabolize of hyperglycemia and misusing glucocorticoids and antibiotic leading to secondary infection are the main reasons for SARS Patients dying.
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PAGE: 585-588
Evaluation of noninvasive Positive Pressure ventilation in treatment for Patients with severe acute respiratory syndrome
CHEN Hong, WANG Xiang ping, LI Fei, YANG Qiang, ZHANG Lian guo, DU Jian xin, ZHAO Song lin
Department of General Surgery Xuanwu Hospital of the Capital University of Medical Sciences, Beijing 100053, China
ABSTRACT: OBJECTIVE: To analyze the effects of noninvasive Positive Pressure ventilation (NIPPV) on oxygenation of severe acute respiratory syndrome (SARS) Patients, and to discuss the timing Point for mechanical ventilation. METHODS: Twenty-five SARS Patients with respiratory dysfunction treated with NIPPV were studied retrospectively in order to evaluate the influences within 24 hours after initiation of ventilatory support on their Physiological indices and oxygenation. Patients with SARS were divided into two groups: survivor group (n=13) and non-survivor group (n=12). We compared the acute Physiology and chronic health evaluation (APACHEII) score, respiratory rate (RR),saturation of oxygen (SPO2) and modificative respiratory index (MRI) for the survivors and non-survivors before NIPPV and after NIPPV for twenty-four hours, respectively. RESULTS: Although NIPPV administered via full-face masks might be an effective treatment for rapidly improving vital signs and gas exchange and sense of dyspnea in both groups during the initial 24 hours of ventilatory support, the Patients in non-survivor group had higher APACHEII score、 respiratory rates and lower SPO2、 MRI than the Patients in survivor group (P<0.05) at the same intervals after initiation of support. CONCLUSION: Noninvasive ventilation should be used as a substitutive tool for endotracheal intubation an alternative treatment for acute respiratory failure related to SARS. Therefore, we should make efforts to avoid missing the time Point for NIPPV or intubation, and we should not be restricted to the available indications for NIPPV or IPPV.
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PAGE: 588
Aualysis of treatment of 22 cases of SARS Patients with mentyl-Prednisolone
ZHOU Yu ling, ZHOU Xiang jun
The Hangzhou Hospital of Chinese People’s Armed Police Forces, Hangzhou 310051, China
PAGE: 589-592
Expression of genes related to inflammatory response in myocardium of rats after burn injury
, 百拇医药
ZHANG Jia ping, HUANG Yu sheng, YANG Zong cheng
Institute of Burn Research, Southwestern Hospital, the Third Military Medical University, Chongqing 400038, China
ABSTRACT: OBJECTIVE: To observe expression of genes related to inflammatory response in myocardium of rats after burn injury. METHODS: After 40 Percent total body surface area (TBSA) of rat was burned gene expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), induced nitric oxide synthase (iNOS) and cytoplastic Phospholipids A2 (cPLA2) in myocardium was detected with reverse transcription-Polymerase chain reaction (RT-PCR) at different time after burn. Meanwhile, changes of contractile and diastolic ability of left ventricle were determined with Physiological recorder. RESULTS: TNF-α and cPLA2 mRNAs were increased dramatically at 1 hour after burn and maintained at high level up to 24 hours later (P<0.01), IL-1β gene expression was up-regulated at 3 hours after burn (P<0.01) and declined to normal level 12 hours later, herein any significant increase of iNOS mRNA in myocardium in rats was not found except decrease in iNOS mRNA could be detected at 1 hour after burn. As expected, severe impairment of myocardiumwas occurred at 3 hours after burn, represented by declining in both contractile and diasotolic ability of lefe ventrical. CONCLUSION: Over expression of TNF-α, cPLA2 and IL-1β mRNA in myocardium in rats may be Partial cause of local myocardial uncontrolled inflammatory response and then Partially contributed to cardiacimpairment after burn.
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PAGE: 593-595
Heat shock Protein 70 induction by heat stress suppress interleukin-1β and tumor necrosis factor-α levels in the brain tissue in rats with infectious brain edema
MAO An ding, YU Pei lan, YANG Yu jia
Department of Neurology, Jiangxi Children Hospital, Nanchang 330006, Jiangxi, China
ABSTRACT : OBJECTIVE: To investigate the changes of interleukin-1β(IL-1β) and tumor necrosis factor-α (TNF-α) in the brain tissue of rats with infectious brain edema (IBE) and their relationship with heat shock Protein 70 (HSP70) by heat stress response (HSR). METHODS: Seventy-two SD rats were randomly divided into normal controls group (NS group), IBE group, and HSP group, each group was divided into three subgroups. The rats in subgroups were killed at 4 hours, 8 hours and 24 hours after the injections of IBE or normal saline respectively. HSP70 in brain tissues were determined by Western blot analysis. The concentrations of IL-1β and TNF-α in the brain homogenate of rats were determined by enzyme linked immunoadsorbent assay (ELISA). RESULTS: The RESULTS showed that HSP70 in brain tissues were elevated after heat shock. IBE group and NS group at 4 hours, 8 hours, 24 hours were induced to base levels of HSP70. The concentrations of TNF-α were significantly elevated in IBE group than in NS group at the various time Points (P<0.01 or P<0.05), especially at 8 hours. The concentrations of IL-1β were significantly increased in IBE group compared with NS group at 4 hours, 8 hours. HSR reduced the IL-1β and TNF-α concentrations in the brain tissue in compared with IBE group (P<0.05 or P<0.01). CONCLUSION: IL-1β and TNF-α are involved in infectious brain edema by IBE. HSP70 against infectious brain edema in rats may be associated with the reduction of IL-1β and TNF-α in brain tissue.
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PAGE: 596-599
Experimental study on recombinant human Platelet-derived growth factor gel in a diabetic rat model of cutaneous incisal wound healing
SUN Tong zhu, FU Xiao bing, ZHAO Zhi li, CHEN Wei, SUN Xiao qing, ZHOU Gang, SHENG Zhi yong
Research Department of Burn Institute, 304th Hospital of People’s Liberation Army, Beijing 100037, China
ABSTRACT: OBJECTIVE: To investigate the effect of recombinant human Platelet-derived growth factor (rhPDGF-BB) on cutaneous incisal wound healing in diabetic rats and this factor does-effect relationship. METHODS: Thirty Wistar diabetic rats were used in this study. Four full-thickness skin wounds of 2.54 cm2 were incised in the back of each rat. The wounds were divided into five groups: three groups were treated with different dosage of rhPDGF-BB gel (14.0 μg/cm2 wound, 7.0 μg/cm2 wound, 3.5 μg/cm2 wound), other two groups were treated with vehicle or untreated, respectively. rhPDGF-BB gel was topically applied to the wounds from 1 to 14 days. The wound healing effect was assessed by the measurements of the wound area, subcutaneous chamber and histological changes of each group. RESULTS: On 14 days, the wounds of the three groups treated with rhPDGF-BB contracted to (0.22±0.30)cm2, (0.05±0.06)cm2, (0.32±0.32)cm2, respectively. The area of the middle dosage groups was significantly smaller than those treated with vehicle (0.23±0.22)cm2 (P<0.05) and untreated (0.22±0.25)cm2 (P<0.05). On 7 days, the subcutaneous chambers of the three treated groups contracted to (0.04±0.03)ml, (0.02±0.02)ml, (0.06±0.03)ml， and there was a statistic significance when comparing middle dosage group with vehicle group(0.06±0.03)ml (P<0.05) and untreated group(0.07±0.05)ml (P<0.05). As for histological examination, much more granulation formation was shown in the wound bed in middle dosage group than that in other four groups on 7 days, and some of the wounds treated with middle dosage were healing on 14 days. CONCLUSION: It suggested that topical application of rhPDGF-BB might improve wound healing in diabetic rats. The effective dosage of rhPDGF-BB is 7.0 μg/cm2 wound.
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PAGE: 599
Significance of noninvasive hemodynamic monitoring in Patient after
LUO Zhong yuan, WANG Bo
Intensive care unit of 117 military Hospital, hangzhou, zhejiang, 310013
PAGE: 600-602
Characteristics of epidermal growth factor and its receptor expression in dermal chronic ulcers
CHEN Wei, FU Xiao bing, SUN Tong zhu, SUN Xiao qing, SUN Peng, ZHAO Zhi li, SHENG Zhi yong
, 百拇医药
304th Hospital of People’s Liberation Army, Beijing 100037, China
ABSTRACT: OBJECTIVE: To investigate the expression and location of epidermal growth factor (EGF) and its receptor (EGFR) in dermal chronic ulcers and normal skin in order to explore their influence on ulcer formation. METHODS: The expression intensity and distribution of EGF and EGFR were detected with Pathological method and immunohistochemistry method in 8 cases of dermal ulcers, 8 cases of edge of ulcer and 8 cases of normal skin. RESULTS: The Positive signals of EGF could be found in epidermal cells, endothelial cells and some fibroblasts; EGFR was principally located in the cytoplasm and cellular membrane of these cells mentioned above in normal skin. From normal skin, edge of ulcer to ulcerative tissues, the Protein contents of EGF and EGFR were decreased progressively. In ulcerative tissues, EGF was mostly distributed in monocytes and macrophages while EGFR was chiefly sited in monocytes. When compared with normal skins, the Protein expression of EGF and EGFR was notably reduced in ulcerative tissues (both P<0.01). The Positive cellular ratios of two Proteins were reduced to (7.1±5.2)% and (8.8±5.5)% of those in normal skin respectively (all P<0.01). CONCLUSION: The formation of dermal chronic ulcers is closely associated with the reduction of EGF and EGFR Protein expression which may lead to binding obstruction between EGF and its receptor.
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PAGE: 603-605
Effects of serum from normal and burned rats on the biological behavior of bone marrow stromal cells
HAN Bing, FU Xiao bing, CHEN Wei, SUN Tong zhu, ZHOU Gang
Key Laboratory of Wound Repair, 304th Hospital of People’s Liberation Army, Beijing 100037, China
ABSTRACT: OBJECTIVE: To investigate the effect of serum from normal and burned rats on the biological behaviors of bone marrow stromal cells (BMSCs). METHODS: Bone marrow was extracted from sacrificed Wistar rats and BMSCs were then incubated in F-12 medium in the Presence of fetal calf serum (FCS, group F), normal rat serum (group N), serum harvested from burned rats at 3 days (group B1) and 14 days (group B2) after burn injury. The growth curve, cell cycle, apoptosis rate and contents of DNA of BMSCs were investigated. RESULTS: The growth curves of cells incubated in different rat serum were similar, but were significantly different from that of cells incubated in FCS. The doubling time of cells incubated in rat serum from group N, B1 and B2 were 23.7, 18.6 and 20.2 hours, respectively, which were significantly shortened compared with that in group F. Compared with group F, the numbers of cells at Platform Phase in group N, B1 and B2 were greater. The ratios of cells at Phase G0/G1 in group B1 and B2 were significantly lower than those in other groups (all P<0.01), but the ratios of cells at Phase S were adverse (P<0.01). Contents of DNA in cells incubated in rat serum were greater than that in cells incubated in FCS (P<0.05 or P<0.01). Contents of DNA in cells incubated in serum from burned rats were greater than that in cells in normal rat serum (P<0.01). The rate of cell apoptosis in group B1 was higher than those in other groups (P<0.01). CONCLUSION: Rat serum is more effective to stimulate Proliferation of BMSCs from rat in vitro than FCS. There are probably many factors affecting the growth of BMSCs, the contents of which changed with the time after burn injury. The effects of serum from burned rats on the growth of BMSCs are complex.
, 百拇医药
PAGE: 606-608
Isolation and culture of multipotent mesenchymal stem cells from Porcine bone marrow
FANG Li jun, FU Xiao bing, SUN Tong zhu, LI Jian fu, CHENG Biao, WANG Yu xin
Department of Stomatology, the Second Hospital of China Medical University, Shenyang 110003, Liaoning, China.
ABSTRACT : OBJECTIVE: To establish a method for the isolation of Porcine mesenchymal stem cells (MSCs) from bone marrow and to demonstrate their differentiation ex vivo into various mesenchymal tissue cells. METHODS: MSCs were isolated from bone marrow and Purified by centrifuge and in vitro. The Proliferation and growth characteristics were observed in Primary and Passage culture. Cell cycle was analyzed by measuring DNA content with FAC-Scan flow cytometer and cell multipotent was identified with specific staining. RESULTS: The adherent，fibroblast-like cells were confluent in single layer after Plating for 12-14 days. The cultured MSCs in vitro differentiated into osteoblasts. The cell cycle analysis showed that 80% of MSCs were in G0/G1 Phase. CONCLUSION: Porcine MSCs can be isolated from postnatal bone marrow through their adherent ability. Porcine MSCs may be introduced as a valuable model system to study the mesenchymal lineages for basic research and tissue engineering.
, 百拇医药
PAGE: 609-611
Changes and effects of group II metabotropic glutamate receptors in the cerebral cortex of rats after diffuse brain injuries coupled with hyperpiesia secondary brain insults
FEI Zhou, ZHANG Xiang, GONG Fang he, BAI Hong min, WANG Xi ling, LIANG Jing wen
Department of Neurosurgery, Xijing Hospital, the Fourth Military Medical University, Xi'an 710033, Shanxi, China
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ABSTRACT: OBJECTIVE: To study the changes and effects of groupIImetabotropic glutamate receptors(mGluR) after diffuse brain injuries (DBI) coupled with hyperpiesia secondary brain insults(SBI). METHODS: Male SD rats were randomized into four groups: normal control, sham-operated, DBI alone and DBI coupled with SBI group. The SBI model was made on the basis of Marmarou's model. The mRNAs of the mGluRs were detected at 1, 3, 6, 12, 24, 48 and 72 hours after injuries by in-situ hybridization, and the Positive neurons were counted. RESULTS: Statistical analysis showed no significance between normal control group and sham-operated group, DBI group in mGluR 2, 3 mRNA (all P>0.05). The number of mGluR 2, 3 Positive neurons decreased at 12 hours after injury and the Peak occurred at 48 hours after injury in the injured cerebral cortex in DBI alone group (both P<0.05). However, in DBI with SBI group, there was a significant decrease of the number of mGluR2, 3 Positive neurons at 6 hours after injury and the Peak happened at 24 hours after injury (both P<0.05). CONCLUSION: mGluR2, 3, factors with the function of protecting brain, might play an important role in the Path Physiology of DBI and SBI.
, 百拇医药
PAGE: 612-614
Changes in Proinflammatory cytokines and immune function of subeschar tissue fluid during early stage after severe burn
RONG Xin zhou, LI Qing hui, HUANG Xiao hua, BEI Chun hua, LIU Zhi qiang, SU Yuan ming
Department of Burns, First Municipal People’s Hospital, Guangzhou 510180, Guangdong, China
ABSTRACT: OBJECTIVE: To evaluate the inflammatory response and immune function of subeschar tissue fluid (STF) in early stage of severe burn. METHODS: Eight Patients were observed and samples from blood as well as STF were collected at 16, 24, 32, 40, 48 hours following burn to measure white blood cell (WBC) counts, levels of immunoglobin (IgA, IgG, IgM), complement (C3, C4) values as well as proinflammatory cytokines as tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) and IL-8. In addition, twenty Balb-c mice were used for the lethal experiment by STF collected at 48 hours after burn. RESULTS: The levels of IgA, IgG, IgM in STF were about half of those in the blood samples (all P<0.01). The WBC counts in STF were 1/100 time of that in the blood (all P<0.01). The contents of TNF-α, IL-6 and IL-8 in STF were comparable with those in the blood (P>0.05). Following STF challenge, none of the mice died during 7-day observation Period. CONCLUSION: The cells and large molecules seems to be more difficult to enter STF compared with small molecules. No marked local inflammatory response occurs in STF during early stage of severe burn, and STF challenge has no lethal effect. The low WBC counts and low immunoglobin as well as complement levels imply the limited effect of STF on local infection, and effective antibiotics should be timely used after major burn.
, 百拇医药
PAGE: 615-617
Dynamic changes of Platelet number in early multiPle injury and it is effect on Prognosis
HAO Jiang, LIE Ming, ZHANG Yin, ZHANG Ping, SUN Chang ju, BIAN Ge yuan, BAI Li
Kunming General Hospital, Chengdu Military Region, Kunming 650032, Yunnan, China
ABSTRACT: OBJECTIVE: To study the dynamic changes of Platelet number in early multiPle injured Patients and its clinical significance. METHODS: Ninety Patients with multiple injuries less than 48 hours were selected. Blood analysis was dynamicly investigated at 1, 24, 72, 120 and 168 hours after in Hospital, and acute Physiology and chronic health evaluationIII (APACHEIII) and injury seriousness scale (ISS) scores were recorded.RESULTS:The Platelet number declined after injury, reached the lowest Point at 72 hours after in Hospital, then restored to the normal level at 120 hours. In the Platelet decrease group, the trauma was more critically [ISS (29.60±9.80) scores vs. (22.30±12.00) scores, (P<0.05)] and the incidence of multiple organs dysfunction syndrome (MODS) was higher than normal group (83.9% vs. 62.7%, (P<0.05). In the death group, the number of Platelet was declined[(30.1±10.3)hours vs. (51.3±14.8) hours, (P<0.05) and the lasting tine was longer [(63.7±11.0) hours vs. (54.2±13.4) hours, (P<0.05). CONCLUSION: The Platelet number declines after multiple injury. The decrease and continuously lower level of Platelet suggest Poor Prognosis.
, 百拇医药
PAGE: 618-621
Change in intestinal free radical and Prostaglandin metabolism in the aged rats with Pneumococcus Pneumonia
LI Jian sheng, LI Su yun, GUO Sheng dian
Geriatrics Department of Henan College of Traditional ChineseMedicine, Zhengzhou 450003, Henan, China.
ABSTRACT: OBJECTIVE: To study the Pathogenic mechanism underlying intestinal injury in the aged rat with Pneumonia. METHODS: The model of rats with Pneumococcus Pneumonia was reproduced, and animals were dividedinto young control group (YCG), young model group (YMG), aged control group (ACG) and aged model group (AMG). The Pathological change of lung tissue and intestine, contents of intestinal 6-keto-Prostaglandin F1α (6-keto-PGF1α), thromboxane B2 (TXB2), nitric oxide (NO), malondialde hyde (MDA) and suPeroxide dismutase (SOD) activity were determined in various groups. RESULTS: The lung and intestinal injury in AMG was more serious than that in the YMG. The decreases in SOD activity and 6-keto-PGF1α content, the increases in contents of TXB2, NO, MDA in the YMG and the AMG were obvious than those in the YCG and the ACG respectively. The decrease in SOD activity and increase in MDA content were obvious in the ACG than those in the YCG. In addition, the decrease in SOD activity and increase in MDA content in the AMG were significant compared with those in the YMG. CONCLUSION: The Prostaglandin metabolism and the free radical injury might be in the Pathogenesis of intestinal injury in the aged rats with Pneumonia. The intestinal injury induced by free radical in aged rats with Pneumonia appears to be more obvious with ageing.
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PAGE: 622-624
Effect of endotoxemia on thyrotropin releasing hormone and its receptor in Wistar rat brain
WANG Xin chun, LIU Bei yan, XU Ping, JING Hui
Emergency Department, Second Hospital Affiliated to Harbin Medical University, Harbin 150086, Heilongjiang, China
ABSTRACT: OBJECTIVE: To study the dose and location of thyrotropin releasing hormone (TRH) and its receptor in Wistar rat's brain when early endotoxemia happened. METHODS: Twenty-two Wistar rats were selected. In 13 mouse the model was established with endotoxemia and hemorrhagic shock. The TRH and its receptor were observed with radioimmunoassay (RIA), and were compared to the 9 control rats. RESULTS: The dose of TRH decreased significantly in the early stage of endotoxemia. The maxium dose (Bmax) of TRH increased while Kd (affinity) decreased, so the effective TRH receptor had no significantly change. CONCLUSION: The dose of TRH is different in brain and serum. The decrease of TRH and the steady of active TRH receptor may protect the brain tissue and repress multiple organ dysfunction syndrome (MODS) in the early stage of endotoxemia.
, 百拇医药
PAGE: 625-627
Change of the erythrocyte chemokine receptor binding activity in scalded rats with Pseudomonas aeruginosa infection
CHEN Xiao dong, WANG Shun bing, WU Bo yu, JIANG Qiong
Provincial Burns Institute, Union Hospital, Fujian Medical University, Fuzhou 350001, Fujian, China.
ABSTRACT: OBJECTIVE: To explore the influence of infection on the erythrocyte chemokine receptor (ECKR)binding activity in severely scalded rats. METHODS: The SPrague-Dawley (SD) rats were randomly divided into three group: sham scald group (A), burn and infection group (B) and infection group (C). The B group rats were scalded with 30% total body surface area (TBSA) of III degree, and the rats ECKR binding activity with interleukin-8 (IL-8) as ligand were detected by enzyme-linked immunosorbent assay (ELISA) at 1, 2, 4, 6 and 8 hours after infection. RESULTS: Compared to that in A group, ECKR binding activities declined significantly (both P<0.01) after infection in both of B and C groups, but they increased at 8 hours (P<0.01). ECKR binding activity in B group was significantly less at 2, 8 hours than that in C group after infection (both P<0.05). The declining range of ECKR binding activity was more in B group, in which the decline of ECKR binding activity appeared earlier (2 hours) than that in C (6 hours) group. CONCLUSION: The infection lead to the decline of ECKR binding activity in burned rats, and the erythrocytes might Participate in the chemokine regulation and Play a novel role in the infection.
, 百拇医药
PAGE: 628-630
Effect of hypotonic liquid containing colloid on hemorrhagic dogs with celiac seawater immersion wound
WU Yin ai, WANG Zhi wei, LIU Xian tang, LUO Zhen dong, YU Hong tao, HU Shi xiong, JIN Hao sheng
General Surgery Department, 157th Hospital of People’s Liberation Army, Guangzhou 510510, Guangdong, China
ABSTRACT: OBJECTIVE: To determine hypotonic liquid containing colloid on hemorrhagic dogs with celiac seawater immersion wound. METHODS: Thirty-five dogs were randomly divided into five groups: control group (group A), 0.9% NaCl therapy group (group B) and 0.45% NaCl therapy group (group C), 5% glucose therapy group(group D) and hypotonic liquid containing colloid therapy group (group E). Changes of mean artery Pressure (MAP), cardiac output (CO), urine volume, Plasma osmotic Pressure and Pathologic changes of lung and brain were observed. RESULTS: hypotonic liquid containing colloid could effectively ameliorate MAP and CO, improve urine volume, decrease Plasma osmotic Pressure and Prevent occurrence of lung and brain edema. CONCLUSION: Hypotonic liquid containing colloid can exert satisfactory therapeutic effects on hemorrhagic dogs with celiac seawater immersion wound.
, 百拇医药
PAGE: 631-633
Risk factors for intracerebral hemorrhage after intravenous thrombolysis in acute cerebral infarction
LI Dou, LEI Yan ni
Complex Internal Medicine, Beijing Emergency Central, Beijing 100031, China
ABSTRACT: OBJECTIVE: To identify risk factors of intracerebral hemorrhage (ICH) after intravenous thrombolysis in acute cerebral infarction. METHODS: Eighty-two Patients with acute cerebral infarction were treated with intravenous urokinase within 12 hours from stroke onset. The data of clinic and laboratory were as readily available baseline factors and were analyzed to select Potential contributors to ICH. RESULTS: ICH occurred in 15 Patients (18.3%), including 6 hemorrhagic infarction (HI, 7.3%) and 9 Parenchyma hemorrhage (PH, 11.0%). Seven Patients had symptomatic ICH, and 4 Patients died. Patients with ICH had significantly lower European stroke scale (ESS) scores. Patients with ESS<60 scores, early ischemic changes (EIC) on cranial CT scans or artrial fibrillation had significantly higher risk of ICH. CONCLUSION: Risk factors associated with ICH are severity of neurological deficit, EIC and artrial fibrillation.
, 百拇医药
PAGE: 634-635
Solid stereotaetic installing tube and drainage with CT in treatment of severe hypertensive cerebrall hemorrhage: report of 32 cases
ZHANG Yue ke, HAN Xian qi, QI Yu jie, LIN Tao fen, YANG Xing yi
Jimo People’s Hospital, Jimo 266200, Shandong
PAGE: 635
Treatments of urethra rupture after simple administration in emergency department
, 百拇医药
LIU Zhi liang, LI Zhan qing, ZHANG Zhi guo, LI Ri qing
Datong the fifth hospital, Datong 037006, Shanxi
PAGE: 636-638
Application of genetic engineering on wound repair
LIU Qiang, CHAI Jia ke
Burns Institute, 304th Hospital of PLA
PAGE: 639-640
European Society of cardiology guideline (III): Percutaneous coronary interventions Emergency department
ZHANG Wei, SHEN Hong
The Emergency Department of the General Hospital of People’s Liberation Army, Beijing 100853, China., http://www.100md.com