研究证实PP2A哪一个亚型发挥肿瘤抑制作用
生物谷报道:最新一期Cancer cell上刊登了一篇文章解决了这个谜,认为PP2A B56γ3 可能是主要的亚型.
The SV40 small t antigen (ST) interacts with the serine-threonine protein phosphatase 2A (PP2A). To investigate the role of this interaction in transformation, we suppressed the expression of the PP2A B56γ subunit in human embryonic kidney (HEK) epithelial cells expressing SV40 large T antigen, hTERT, and H-RAS. Suppression of PP2A B56γ expression inhibited PP2A-specific phosphatase activity similar to that achieved by ST and conferred the ability to grow in an anchorage-independent fashion and to form tumors. Overexpression of PP2A B56γ3 in tumorigenic HEK cells expressing ST or human lung cancer cell lines partially reversed the tumorigenicity of these cells. These observations identify specific PP2A complexes involved in human cell transformation.
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The SV40 small t antigen (ST) interacts with the serine-threonine protein phosphatase 2A (PP2A). To investigate the role of this interaction in transformation, we suppressed the expression of the PP2A B56γ subunit in human embryonic kidney (HEK) epithelial cells expressing SV40 large T antigen, hTERT, and H-RAS. Suppression of PP2A B56γ expression inhibited PP2A-specific phosphatase activity similar to that achieved by ST and conferred the ability to grow in an anchorage-independent fashion and to form tumors. Overexpression of PP2A B56γ3 in tumorigenic HEK cells expressing ST or human lung cancer cell lines partially reversed the tumorigenicity of these cells. These observations identify specific PP2A complexes involved in human cell transformation.
Related articles:
Identification of specific PP2A complexes involved in human cell transformationWen Chen, Richard Possemato, K. Thirza Campbell, Courtney A. Plattner, David C. Pallas, and William C. Hahn
[Summary] [Full Text] [PDF]
PP2A fulfills its promises as tumor suppressor: Which subunits are important?Christine Van Hoof and Jozef Goris
[Summary] [Full Text] [PDF]
Protein hijacking: Key proteins held captive against their willAna Traven, David C.S. Huang, and Trevor Lithgow
[Summary] [Full Text] [PDF], 百拇医药