经颅重频磁刺激对大鼠脑缺血再灌流损伤后 运动皮层兴奋性和神经功能的影响
北京协和医院神经内科 (100730)
【摘要】目的：观察经颅重频磁刺激(rTMS)对大鼠脑缺血再灌流损伤后运动皮层兴奋性和神经功能的影响。方法：测定清醒状态下SD大鼠右后肢运动阈值(MT)和运动诱发电位(MEP)，制作左侧大脑中动脉栓塞再流模型，并给予rTMS，1次/ d，一周后再次测定MT、MEP，进行神经功能评分，处死测量脑梗死体积。观察rTMS对损伤前、后MT、MEP、以及损伤后神经功能评分、梗死体积的影响。结果：脑缺血再灌流损伤使大鼠MT升高，神经功能评分增加，形成明确的局灶性梗死灶，对MEP波幅、潜伏期无显著影响。rTMS治疗可降低大鼠MT和神经功能评分升高的程度，维持神经功能，与对照组相比有统计学显著性差异(P＝0.02，0.03)；并减小梗死体积，但与对照组相比无统计学显著性差异(P＝0.06)。结论： rTMS可能促进脑缺血再灌流损伤后神经功能的恢复，有进一步的研究应用前景。
【关键词】经颅重频磁刺激；脑缺血再灌流损伤；运动阈值；运动诱发电位
Effects of repetitive transcranial magnetic
stimulation on rats’ motor cortical excitability and neurofunction after cerebral ischemia-reperfusion injury
CUI Liying YAN Li FENG Honglin
【Abstract】Objective: To clarify the effects of repetitive transcranial magnetic stimulation (rTMS) on rats’ motor cortical excitability and neurofunction after cerebral ischemia-reperfusion injury. Methods: After determined awake motor threshold (MT) and motor evoked potential (MEP) of right hindlimbs, Sprague-Dawley rats were subjected to the middle cerebral artery occlusion and reperfusion injury, then rTMS were applied to them once a day. A week later, MT and MEP were evaluated again, as well as the extent of neurologic deficits graded as different scores using two examinations and infarct volume. The variations of these parameters and the effects of rTMS were analyzed. Results: After cerebral ischemia-reperfusion, both of MT and neurologic deficient scores increased and distinct focal infarction formed. The change of amplitude and latency of MEP was not evident. On the other hand, when compared with control group, the MT and neurological scores of rats receiving rTMS had been improved significantly (P=0.02, 0.03); the infarct volume decreased by 37%, but there is no statistically significance (P=0.06). Conclusion: rTMS may facilitate neurofunction recovery after cerebral ischemia-reperfusion injury, and should be explored further.
【Key words】 repetitive transcranial magnetic stimulation cerebral ischemia-reperfusion injury motor threshold motor evoked potential, 百拇医药(崔丽英 严莉 丰宏林)
【摘要】目的：观察经颅重频磁刺激(rTMS)对大鼠脑缺血再灌流损伤后运动皮层兴奋性和神经功能的影响。方法：测定清醒状态下SD大鼠右后肢运动阈值(MT)和运动诱发电位(MEP)，制作左侧大脑中动脉栓塞再流模型，并给予rTMS，1次/ d，一周后再次测定MT、MEP，进行神经功能评分，处死测量脑梗死体积。观察rTMS对损伤前、后MT、MEP、以及损伤后神经功能评分、梗死体积的影响。结果：脑缺血再灌流损伤使大鼠MT升高，神经功能评分增加，形成明确的局灶性梗死灶，对MEP波幅、潜伏期无显著影响。rTMS治疗可降低大鼠MT和神经功能评分升高的程度，维持神经功能，与对照组相比有统计学显著性差异(P＝0.02，0.03)；并减小梗死体积，但与对照组相比无统计学显著性差异(P＝0.06)。结论： rTMS可能促进脑缺血再灌流损伤后神经功能的恢复，有进一步的研究应用前景。
【关键词】经颅重频磁刺激；脑缺血再灌流损伤；运动阈值；运动诱发电位
Effects of repetitive transcranial magnetic
stimulation on rats’ motor cortical excitability and neurofunction after cerebral ischemia-reperfusion injury
CUI Liying YAN Li FENG Honglin
【Abstract】Objective: To clarify the effects of repetitive transcranial magnetic stimulation (rTMS) on rats’ motor cortical excitability and neurofunction after cerebral ischemia-reperfusion injury. Methods: After determined awake motor threshold (MT) and motor evoked potential (MEP) of right hindlimbs, Sprague-Dawley rats were subjected to the middle cerebral artery occlusion and reperfusion injury, then rTMS were applied to them once a day. A week later, MT and MEP were evaluated again, as well as the extent of neurologic deficits graded as different scores using two examinations and infarct volume. The variations of these parameters and the effects of rTMS were analyzed. Results: After cerebral ischemia-reperfusion, both of MT and neurologic deficient scores increased and distinct focal infarction formed. The change of amplitude and latency of MEP was not evident. On the other hand, when compared with control group, the MT and neurological scores of rats receiving rTMS had been improved significantly (P=0.02, 0.03); the infarct volume decreased by 37%, but there is no statistically significance (P=0.06). Conclusion: rTMS may facilitate neurofunction recovery after cerebral ischemia-reperfusion injury, and should be explored further.
【Key words】 repetitive transcranial magnetic stimulation cerebral ischemia-reperfusion injury motor threshold motor evoked potential, 百拇医药(崔丽英 严莉 丰宏林)