大鼠激光心肌血管重建术后血管新生与血管生长因子的变化(摘要)
作者：黄璇 郭静萱 张萍
单位：北京市，北京医科大学第三医院 心内科(100083)
关键词：
中国循环杂志99zk42 目的：观察TMR术后新生血管的变化及血管内皮细胞生长因子(VEGF)及碱性成纤维细胞生长因子(bFGF)在缺血心肌的表达，揭示TMR的可能机制。
方法：制作大鼠急性心肌缺血模型，以单纯缺血为对照组，实验组行激光打孔。于术后不同时间(3天、1周、2周、4周、6周、8周)观察新生血管的变化情况，用免疫组化方法观察血管内皮细胞生长因子(VEGF)和碱性成纤维细胞生长因子的变化。
结果：实验组2周时可见新生的毛细血管，随时间逐渐增多。实验组孔道区在术后3天即有VEGF表达，在4周时开始上升，于6周时达高峰，8周开始下降，而对照组仅在4周时有VEGF的表达，随之逐渐减少；实验组bFGF在前4周无明显表达，于第6周时表达显著增加，达高峰后于8周时呈下降趋势，对照组仅于2周及4周时有少量表达，于6周后逐渐消失。
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结论：TMR术后2周开始出现新生的毛细血管，单纯缺血组(对照组)未见明显的血管新生现象。TMR术后VEGF、bFGF表达较对照组明显增加，于6周时表达量最多，范围最广，8周时呈下降趋势。TMR术可通过介导致血管生成的生长因子(如VEGF及bFGF)的释放来达到其部分血管新生的作用。
Vascular Growth and Expression of Growth Factor after Transmyocardial Laser Revascularization (Abstract)
Department of Cardiology, The Third Hospital, Beijing Medical University, Beijing (100083)
Huang Xuan, Guo Jingxuan, Zhang Ping.
, 百拇医药
Objective: To observe the new vessel growth and the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) after transmyocardial laser revascularization (TMR) and to investigate the relationship between TMR, growth factor and angiogenesis.
Methods: Eighty-four Wistar rats underwent ligation of the left coronary artery. They were randomly divided into two groups. One (n=42) served as controls, whereas the other (n=42) underwent concomitant TMR by the creation of three transmural channels with modulating Nd: YAG laster on left ventricular free wall. Then the animals were sacrificed at intervals of 3 days, 1, 2, 4, 6 and 8 weeks (n=7 in each subgroup). HE staining was used to observe the vascular growth. Immunohistochemistry using specific poly-colonal antibodies was performed against VEGF and bFGF. Growth factor expression was quantitated by comparing optical density (OD) of staining with computerized morphometric analysis.
, http://www.100md.com
Results: Mortality was similar in both groups. TMR group showed capillary growth beginning at week 2 and thereafter gradually increased till week 8. TMR group had significant higher VEGF expression than controls in all subgroups with a peak at week 6 postoperatively (0.062±0.004 vs. 0.198±0.022; p<0.001). No significant differences in bFGF expression were found between two groups at day 3 and week 1. It began to increase at week 2 in controls and reduced thereafter. In TMR group, it peaked at week 6. Level of bFGF were significant higher in TMR group compared with the control group at week 6 and 8 (0.118±0.017 vs. 0.233±0.012, p<0.001; 0.113±0.034 vs. 0.162±0.010, p<0.001).
, 百拇医药
Conclusion: The expression of VEGF and bFGF is significantly increased after TMR procedure with vascular growth beginning at week 2 in the model of acute myocardial ischemia in rats. It appears that the angiogenic response is mediated by the release of certain angiogenic growth factors such as VEGF and bFGF. With the increasing recognition that the long-term patency of myocardial channels created by laser does not exist, its mechanism may be related to the effect of angiogenesis., 百拇医药
单位：北京市，北京医科大学第三医院 心内科(100083)
关键词：
中国循环杂志99zk42 目的：观察TMR术后新生血管的变化及血管内皮细胞生长因子(VEGF)及碱性成纤维细胞生长因子(bFGF)在缺血心肌的表达，揭示TMR的可能机制。
方法：制作大鼠急性心肌缺血模型，以单纯缺血为对照组，实验组行激光打孔。于术后不同时间(3天、1周、2周、4周、6周、8周)观察新生血管的变化情况，用免疫组化方法观察血管内皮细胞生长因子(VEGF)和碱性成纤维细胞生长因子的变化。
结果：实验组2周时可见新生的毛细血管，随时间逐渐增多。实验组孔道区在术后3天即有VEGF表达，在4周时开始上升，于6周时达高峰，8周开始下降，而对照组仅在4周时有VEGF的表达，随之逐渐减少；实验组bFGF在前4周无明显表达，于第6周时表达显著增加，达高峰后于8周时呈下降趋势，对照组仅于2周及4周时有少量表达，于6周后逐渐消失。
, http://www.100md.com
结论：TMR术后2周开始出现新生的毛细血管，单纯缺血组(对照组)未见明显的血管新生现象。TMR术后VEGF、bFGF表达较对照组明显增加，于6周时表达量最多，范围最广，8周时呈下降趋势。TMR术可通过介导致血管生成的生长因子(如VEGF及bFGF)的释放来达到其部分血管新生的作用。
Vascular Growth and Expression of Growth Factor after Transmyocardial Laser Revascularization (Abstract)
Department of Cardiology, The Third Hospital, Beijing Medical University, Beijing (100083)
Huang Xuan, Guo Jingxuan, Zhang Ping.
, 百拇医药
Objective: To observe the new vessel growth and the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) after transmyocardial laser revascularization (TMR) and to investigate the relationship between TMR, growth factor and angiogenesis.
Methods: Eighty-four Wistar rats underwent ligation of the left coronary artery. They were randomly divided into two groups. One (n=42) served as controls, whereas the other (n=42) underwent concomitant TMR by the creation of three transmural channels with modulating Nd: YAG laster on left ventricular free wall. Then the animals were sacrificed at intervals of 3 days, 1, 2, 4, 6 and 8 weeks (n=7 in each subgroup). HE staining was used to observe the vascular growth. Immunohistochemistry using specific poly-colonal antibodies was performed against VEGF and bFGF. Growth factor expression was quantitated by comparing optical density (OD) of staining with computerized morphometric analysis.
, http://www.100md.com
Results: Mortality was similar in both groups. TMR group showed capillary growth beginning at week 2 and thereafter gradually increased till week 8. TMR group had significant higher VEGF expression than controls in all subgroups with a peak at week 6 postoperatively (0.062±0.004 vs. 0.198±0.022; p<0.001). No significant differences in bFGF expression were found between two groups at day 3 and week 1. It began to increase at week 2 in controls and reduced thereafter. In TMR group, it peaked at week 6. Level of bFGF were significant higher in TMR group compared with the control group at week 6 and 8 (0.118±0.017 vs. 0.233±0.012, p<0.001; 0.113±0.034 vs. 0.162±0.010, p<0.001).
, 百拇医药
Conclusion: The expression of VEGF and bFGF is significantly increased after TMR procedure with vascular growth beginning at week 2 in the model of acute myocardial ischemia in rats. It appears that the angiogenic response is mediated by the release of certain angiogenic growth factors such as VEGF and bFGF. With the increasing recognition that the long-term patency of myocardial channels created by laser does not exist, its mechanism may be related to the effect of angiogenesis., 百拇医药