抗氧化剂对大鼠颈动脉损伤后血管内膜增殖的影响(摘要)
作者：裴诚民 陈星
单位：天津市，天津胸科医院 心内科(300051)
关键词：
中国循环杂志99zk41 目的：动脉血管内膜增厚造成血管腔有效面积减少是经皮冠状动脉腔内成形术(PTCA)术后再狭窄甚至动脉粥样硬化的主要病理基础，其中血管平滑肌细胞(VSMC)过度增殖是其主要原因。因此，抑制VSMC过度增殖是防治再狭窄的首要任务。本研究观察了抗氧化剂Vit E对胆固醇饲喂的大鼠颈动脉损伤后血管内膜增殖的影响效应。
方法：将24只SD大鼠随机分成0.5% Vit E+1%胆固醇组，1%胆固醇组和普通饲料组，每组8只大鼠，前两组大鼠在接受后8周，血清胆固醇水平均较前明显升高(P<0.05)。3组大鼠均接受外科手术：用2F Fogarty球囊导管将大鼠单侧颈外动脉内膜剥脱，术后12天处杀动物，内灌注固定动脉后，取受损侧颈动脉做病理切片和免疫组化染色。
, http://www.100md.com
结果：普通饲料组动脉血管内膜平均厚度和内膜/中膜厚度比率(41.5±5.0 μm,0.70±0.09)较1%胆固醇组(43.5±6.0 μm,0.83±0.07)无明显差别(P>0.05);而0.5% Vitamin E+1%胆固醇组动脉血管内膜平均厚度和内膜/中膜厚度比率(26.5±5.0 μm,0.56±0.07)分别明显小于1%胆固醇组和普通饲料组(P<0.05)。动脉内膜PCNA免疫染色阳性率，0.5% Vitamin E+1%胆固醇组和普通饲料组[(11.6±1.1)%,(12.1±2.1)%]明显地低于1%胆固醇组[(17.1±2.4)%,P<0.05];动脉中膜PCNA免疫染色阳性率，0.5% Vitamin E+1%胆固醇组和普通饲料组[(0.40±0.11)%,(0.31±0.16)%]明显地低于1%胆固醇组[(0.81±0.21)%,P<0.05]。所有动脉病理切片(HE染色)均显示，动脉壁新生内膜主要成分为VSMC伴少量巨噬细胞和单核细胞。
结论：动物摄取一定量的Vitamin E可以明显地抑制血管损伤后的内膜过度增殖反应，这种反应主要是由于VSMC过度增殖所致，抗氧化剂Vitamin E可能具有直接抑制VSMC在动脉新生内膜堆积的作用。
, 百拇医药
Antioxidant Inhibits The Intimal Response to Balloon Injury in Rat Carotid Artery (Abstract)
Department of Cardiology, Tianjin Chest Hospital, Tianjin (300051)
Pei Chengmin, Chen Xing.
Objective: The accumulation of vascular smooth muscle cells (VSMCs) within the arterial intima is a key process in the pathogenesis of atherosclerosis. This is also one of the mechanisms leading to restenosis of coronary arteries following PTCA. We have investigated the effect of the naturally occuring, lipid-soluble antioxidant, vitamin E on the intimal response to balloon injury in the cholesterol-fed rats.
, 百拇医药
Methods: Twenty-four rats were assigned to 3 dietary groups at random: Ⅰ 1% cholesterol; Ⅱ 1% cholesterol with 0.5% vitamin E, and Ⅲ control chow. Water was allowed ad libitum. Balloon catheter deendotheliosation of the left common carotid artery was peformed by 3 passages of an inflated 2 F Fogarty embolectomy catheter 8 weeks after starting the experimental diet. 12 days after the procedure, rats were killed by an overdose anaesthetic, and then perfused with paraformaldehyde to in situ fix the arteries. After embedding with paraffin wax. Sections were cut and stained with HE and immunohistochemstry.
, 百拇医药
Results: 1% cholesterol diet caused a significant increase in serum cholesterol in all rats on this diet compared to controls by the time to surgical treatment (p<0.05). Neo-intimal thickness and intimal/medial ratio in control chow group did not differ significantly from the rats receiving 1% cholesteroldiet. These parameters were significantly lower in 1% choelsterol with 0.5% vitamin E group as compared to control and 1% cholesterol groups, respectively (p<0.05). PCNA-positive cells in both 1% cholesterol with 0.5% vitamin E and control groups were fewer than that in 1% cholesterol group (p<0.05). Lesions in affected groups were predominantly composed of VSMCs, a few monocytes/macrophages were observed, either.
, 百拇医药
Conclusion: These data suggested that the reactive oxygen species may be involved in the intimal response to balloon injury, and that antioxidants, such as vitamin E may offer some protection against restenosis. Although the way by which it does so is unclear, one possible mechanism is that by a direct inhibitory effect on the accumulation of VSMCs with the developing neo-intima., 百拇医药
单位：天津市，天津胸科医院 心内科(300051)
关键词：
中国循环杂志99zk41 目的：动脉血管内膜增厚造成血管腔有效面积减少是经皮冠状动脉腔内成形术(PTCA)术后再狭窄甚至动脉粥样硬化的主要病理基础，其中血管平滑肌细胞(VSMC)过度增殖是其主要原因。因此，抑制VSMC过度增殖是防治再狭窄的首要任务。本研究观察了抗氧化剂Vit E对胆固醇饲喂的大鼠颈动脉损伤后血管内膜增殖的影响效应。
方法：将24只SD大鼠随机分成0.5% Vit E+1%胆固醇组，1%胆固醇组和普通饲料组，每组8只大鼠，前两组大鼠在接受后8周，血清胆固醇水平均较前明显升高(P<0.05)。3组大鼠均接受外科手术：用2F Fogarty球囊导管将大鼠单侧颈外动脉内膜剥脱，术后12天处杀动物，内灌注固定动脉后，取受损侧颈动脉做病理切片和免疫组化染色。
, http://www.100md.com
结果：普通饲料组动脉血管内膜平均厚度和内膜/中膜厚度比率(41.5±5.0 μm,0.70±0.09)较1%胆固醇组(43.5±6.0 μm,0.83±0.07)无明显差别(P>0.05);而0.5% Vitamin E+1%胆固醇组动脉血管内膜平均厚度和内膜/中膜厚度比率(26.5±5.0 μm,0.56±0.07)分别明显小于1%胆固醇组和普通饲料组(P<0.05)。动脉内膜PCNA免疫染色阳性率，0.5% Vitamin E+1%胆固醇组和普通饲料组[(11.6±1.1)%,(12.1±2.1)%]明显地低于1%胆固醇组[(17.1±2.4)%,P<0.05];动脉中膜PCNA免疫染色阳性率，0.5% Vitamin E+1%胆固醇组和普通饲料组[(0.40±0.11)%,(0.31±0.16)%]明显地低于1%胆固醇组[(0.81±0.21)%,P<0.05]。所有动脉病理切片(HE染色)均显示，动脉壁新生内膜主要成分为VSMC伴少量巨噬细胞和单核细胞。
结论：动物摄取一定量的Vitamin E可以明显地抑制血管损伤后的内膜过度增殖反应，这种反应主要是由于VSMC过度增殖所致，抗氧化剂Vitamin E可能具有直接抑制VSMC在动脉新生内膜堆积的作用。
, 百拇医药
Antioxidant Inhibits The Intimal Response to Balloon Injury in Rat Carotid Artery (Abstract)
Department of Cardiology, Tianjin Chest Hospital, Tianjin (300051)
Pei Chengmin, Chen Xing.
Objective: The accumulation of vascular smooth muscle cells (VSMCs) within the arterial intima is a key process in the pathogenesis of atherosclerosis. This is also one of the mechanisms leading to restenosis of coronary arteries following PTCA. We have investigated the effect of the naturally occuring, lipid-soluble antioxidant, vitamin E on the intimal response to balloon injury in the cholesterol-fed rats.
, 百拇医药
Methods: Twenty-four rats were assigned to 3 dietary groups at random: Ⅰ 1% cholesterol; Ⅱ 1% cholesterol with 0.5% vitamin E, and Ⅲ control chow. Water was allowed ad libitum. Balloon catheter deendotheliosation of the left common carotid artery was peformed by 3 passages of an inflated 2 F Fogarty embolectomy catheter 8 weeks after starting the experimental diet. 12 days after the procedure, rats were killed by an overdose anaesthetic, and then perfused with paraformaldehyde to in situ fix the arteries. After embedding with paraffin wax. Sections were cut and stained with HE and immunohistochemstry.
, 百拇医药
Results: 1% cholesterol diet caused a significant increase in serum cholesterol in all rats on this diet compared to controls by the time to surgical treatment (p<0.05). Neo-intimal thickness and intimal/medial ratio in control chow group did not differ significantly from the rats receiving 1% cholesteroldiet. These parameters were significantly lower in 1% choelsterol with 0.5% vitamin E group as compared to control and 1% cholesterol groups, respectively (p<0.05). PCNA-positive cells in both 1% cholesterol with 0.5% vitamin E and control groups were fewer than that in 1% cholesterol group (p<0.05). Lesions in affected groups were predominantly composed of VSMCs, a few monocytes/macrophages were observed, either.
, 百拇医药
Conclusion: These data suggested that the reactive oxygen species may be involved in the intimal response to balloon injury, and that antioxidants, such as vitamin E may offer some protection against restenosis. Although the way by which it does so is unclear, one possible mechanism is that by a direct inhibitory effect on the accumulation of VSMCs with the developing neo-intima., 百拇医药