银杏内酯B对脑缺血-再灌注神经元损伤的保护作用
秦 兵，张根葆，陈冬云，许 敏，李爱华(安徽省芜湖市皖南医学院病理生理教研室，安徽 芜湖 241001)
【摘要】 目的：研究银杏内酯B对大鼠脑缺血-再灌注神经元损伤的干预作用及其可能机制。方法：采用Kameyama的3条动脉夹闭法制备大鼠脑缺血-再灌注损伤模型。45只Wistar大鼠随机分成假手术对照组、缺血-再灌注模型组、生理盐水对照组和银杏内酯B预处理组；测定各组动物脑匀浆液中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和ATP酶活性以及丙二醛(MDA)含量，并观察脑组织的病理学改变和细胞凋亡指数。结果：全脑缺血-再灌注可引起SOD、GSH-Px和ATP酶活性明显下降，MDA含量显著升高；脑组织呈现炎症性改变，层次不清，细胞凋亡指数达(40.2±6.3)%。银杏内酯B(1~10 mg/kg)能剂量依赖性地降低MDA水平，提高SOD、GSH-Px和ATP酶活性(P<0.05或P<0.01)，明显减轻脑组织神经细胞损害，减少细胞凋亡发生率。结论：银杏内酯B对大鼠脑缺血-再灌注神经元损伤具有明显的保护作用，其机制可能是通过减少自由基的产生和增强ATP酶活性。
【关键词】 银杏内酯B;缺血-再灌注损伤，脑;自由基;细胞凋亡
Protective effects of ginkgolide B(银杏内酯B) on neuron injury induced by cerebral ischemia/reperfusion
QIN Bing, ZHANG Gen-bao, CHEN Dong-yun， XU Min, LI Ai-hua. Department of Pathophysiology, Wannan Medical College, Wuhu 241001, Anhui, China
Corresponding author: ZHANG Gen-bao
【Abstract】 Objective： To investigate the protective effects and mechanism of ginkgolide B(银杏内酯B, GB) on neuron injury induced by cerebral ischemia/reperfusion(I/R) in rats. Methods： The bilateral cerebral hemisphere I/R model was established by ligating three arteries with Kameyama's manner. Forty-five Wistar rats were randomly assigned to sham operation control, I/R model, normal saline control (NS) and GB pretreatment group. The activity of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), ATPase, and the contents of malondialdehyde(MDA) in brain tissue were measured. Apoptosis of neurons and pathologic changes were observed. Results： Compared with sham operation control group ......
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