项目负责人:孙自勤，1958年9月生。消化内科主治医师，硕士学位。主要研究课题有急性肝损伤的机制与治疗，肝纤维化的诊断、防治等实验与临床研究。[山东省济南市师范路98号(250031)， 电话：552171 转69616]

    摘要目的：汉防己甲素(Tet) 为中药粉防己根的提取物。本研究的目的为评价其抗肝纤维作用。

    方法：用40% CCl₄ 1.2mL/kg体重皮下注射，2次/wk，共12wk，诱发SD大鼠肝纤维化。于初次注射开始，用Tet(治疗组)或盐水(对照组)灌胃，隔日1次共12wk。分别于4,8, 12, 14wk处死动物测定血清I型前胶原(PC)、肝及血清透明质酸(HA)、胶原、储脂细胞(FSC)及其细胞器。

    结果：Tet可使血清PC, HA及肝HA降低，肝内胶原沉积减少。治疗结束时，血清PC为61.8μg/L±21.0μg/L，而对照组为270.2μg/L±46.1μg/L(P<0.01)；血清HA分别为204.0μg/L±70.8μg/L与565.2μg/L±161.9μg/L(P<0.01)；肝组织HA为86.9μg/g±15.3μg/g与186.5μg/g±46.2μg/g(P<0.01)。FSC及其内织网与线粒体受抑。

    结论：我们的实验结果显示Tet能抑制肝内胶原的沉积，可用于治疗慢性肝病之肝纤维化。

    主题词 肝纤维化 汉防己甲素 型前胶原 透明质酸 储脂细胞 治疗

    孙自勤, 王要军, 权启镇, 刘晓峰, 潘雪, 江学良.汉防己甲素对大鼠实验性肝纤维化的防治作用.新消化病学杂志,1994;2(1):19-20

    AIM The effect of tetrandrine (Tet), a purified extract from Chinese herb Stephania tetrandra S Moore, on experimental liver fibrosis in rats was studied.

    METHODS Hepatofibrosis model was induced in SD rats, by 40% CCl₄, 1.2mL/kg body weight, subscut aneously,twice a week for 12 weeks From the first injection of CCl₄ on, 30mg of Tet (treatment group) or saline (control group)was given by stomach every 2 days for 12 weeks. The animals were sacrificed at4, 8, 12 and 14 weeks respectively Serum procollagen type (PC), liver and serum hyaluronate (HA) were detected.The liver collagen,number of fat-storing cells (FSC) and its oranganelles were examined. ......