HBV转基因小鼠暴露ATB-1后肝脏ATB-1-DNA加成物水平的测定
肝,化学黄曲霉素B1肝炎，乙型动物，转基因DNA加成物肝肿瘤,病因学,项目负责人,DetectionofhepaticaflatoxinB,1-DNAadductsinHBVtransgenicmouseafterexposuretoaflatoxinB,1,Abstract,AIM,METHODS,RESULTS,CONCLUSION,Sub
1上海医科大学肿瘤医院中西医结合科 上海市 2000322上海市肿瘤研究所化学致癌室
项目负责人 吴万垠,收搞日期 接受日期:
Detection of hepatic aflatoxin B1-DNA adducts in HBV transgenic mouse after exposure to aflatoxin B1
Wan-Yin Wu1, Yan-Feng Li2, Ming-Zhi Song1, Geng-Sun Qian2 and Er-Xin Yu1
1Department of TCM & Western Medicine, Cancer Hospital of Shan ghai Medical University, Shanghai 200032, China
2Department of Chemical Carcinogenesis, Shanghai Cancer Institute, Shanghai 200032, China
AbstractAIM To elucidate the mechanism of synergistic interaction be tween HBV and aflatoxin B1 (ATB1) on hepatocarcinogenesis.
METHODS With a competitive radioimmunoassay, seven different ti me phases of hepatic ATB1-DNA adducts were detected in HBV transgenic mice an d normal mice at 0, 0.5, 1, 2, 4, 8, 24 hours after exposure to ATB1.
RESULTS The levels of hepatic ATB1-DNA adducts in HBV transgenic mice were higher than those in normal mice, especially remarkable at 1 hour (559.2±41.5 vs 413.6±28.2pmol/mg DNA, P<0.001) and at 24 hours (248.7±20.3 vs 98.9±8.5pmol/mg DNA, P<0.01) after exposure to ATB1. At 24 hours after treated intraperitoneally with ATB1, the level of hepatic ATB1-DNA adducts in HBV transgenic mice was still high, but in normal mice it almost returned to the level before exposure to ATB1. ......
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