钙离子拮抗剂等对成纤维细胞增殖的影响
肝硬变,病理学钙通道拮抗剂成纤维细胞,项目负责人,Effectsofcalciumantagonistsonproliferationoffibroblasts,Abstract,AIM,METHODS,RESULTS,CONCLUSION,Subjectheadings,摘,要,目的,方法,结果,结论,主题词,1,材料和方法,1.1,材料,1.2方
上海第二医科大学附属新华医院消化内科 上海市 200092徐芹芳，女，1954-02-19生，上海市人，汉族. 1973年新华卫校毕业，检验师，主要从事肝纤维化发病机理的研究，发表论文1篇.
项目负责人 徐芹芳，上海市杨浦区控江路1665号.
Tel: 021-65454630-4277收搞日期 1996-05-03 接受日期 1996-09-02
Effects of calcium antagonists on proliferation of fibroblasts
Qin-Fang Xu, Xue-Song Liu, Ding-Guo Li and Han-Ming Lu
Department of Gastroenterology, Xinhua Hospital, Shanghai 200092, China
Abstract
AIM To study the effects of Cin, Nif, A23187 and CPZ on the proliferation of 3T6 fibroblasts.
METHODS 3T6 cells were cultured in RPMI 1640 medium supplemented with 10% FCS. Three different concentrations of Cin, Nif, A-23187 and CPZ were added separately to each test sample, and 24 hours later 3T6 cells were measured by ICM and FCM to determine the DNA, RNA, total protein content and cell cycle.
RESULTS Nif could delay the progression of 3T6 cells from G2 to M phase in a dosedependent manner. Nif, A23187 and CPZ accelerated the synthesis of RNA and Cin inhibited the synthesis of RNA and DNA. A23187 and CPZ (10μg) inhibited the synthesis of protein, but Cin (30μg) and CPZ (5μg) promoted the synthesis of protein. ......
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