TNFα诱导人大肠癌Lovo细胞凋亡的时间效应
鍏抽敭璇嶏細肿瘤坏死因子,药理学;脱噬作用,药物作用;结肠肿瘤,病理学;直肠肿瘤,病理学,项目负责人,Correspondenceto,TimeeffectofTNF,αonapoptosisofLovocellsofhumancolorectalcarcinoma,Abstract,,AIM,METHODS,RESULTS,CONCLUSION,Sub
1空军广州医院小儿内科 广东省广州市 510602
2第一军医大学病理学教研室 广东省广州市 510515
梁卫江,女,1963-03-22生,广东省连州市人,汉族. 1984年第一军医大学医疗系本科毕业,硕士学位,主治医师. 现为第一军医大学南方医院消化病研究所博士研究生,发表论文6篇.
项目负责人 梁卫江,510602,广东省广州市空军广州医院小儿内科.
Correspondence to Wei-Jiang Liang, Department of Internal Medicine, Guangzhou Air Force Hospital, Guangzhou 510602, China
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收稿日期 1997-08-01
Time effect of TNFα on apoptosis of Lovo cells of human colorectal carcinoma
Wei-Jiang Liang1, Zhuo-Yuan Huang2 and Yan-Qing Ding2
Department of Pathology, the First Military Medical University, Guangzhou 510515, Guangdong Province, China
Abstract
AIM To explore the possibility and regulation of apoptosis Lovo cells of human colorectal carcinoma induced by tumor necrosis factorα(TNFα).
METHODS After Lovo cells were cultured in vitro and treated with TNFα1×108U/L for 0 to 48 hours, the morphological changes of apoptosis were observed by HE and Hoechst 33258 dyeing under optical microscope and the percentage of apoptosis was counted, and DNA fragments were detected with DNA electrophoresis. ......
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