干扰素对小鼠α2(Ⅰ)型胶原基因启动子活性的影响*
干扰素α,药理学;干扰素Ⅱ型,药理学;启动区(遗传学),药物作用;溶胶原,遗传学;纤维变性,药物疗法;3T3细胞,基金资助*国家自然科学基金资助项目,No.39500068.,项目负责人,*ProjectsupportedbytheNationalNaturalScienceFoundation
第二军医大学附属长征医院临床免疫中心 上海市 200003高春芳,1993年毕业于第二军医大学获博士后学位,1995年赴德国Marburg philipps大学医院中心实验室工作,现在第二军医大学附属长征医院临床免疫中心工作. 近10年来长期从事有关肝纤维化形成机制的细胞学和分子生物学研究,于国内外杂志发表相关研究论文多篇.
基金资助 *国家自然科学基金资助项目,No. 39500068.
项目负责人 高春芳,200003,第二军医大学附属长征医院临床免疫中心,上海市凤阳路415号.
*Project supported by the National Natural Science Foundation of China, No. 39500068.
Correspondence to Chun-Fang Gao, Clinical Immunology Center, Changzheng Hospital, Second Military Medical
University, 415 Fengyanglu, Shanghai 200003, China
Tel. +86·21·63610109-73636
收稿日期 1997-12-05
IFN effect on promoter activity in mouse α2(Ⅰ) procollagen gene*
Chun-Fang Gao, Hao Wang and Xian-Tao Kong
Clinical Immunology Center, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
Abstract
AIM To study the effect of interferon on the promoter activity in mouse α2(Ⅰ) [WTXFZ]procollagen gene.
METHODS The upper stream 400bp, 800bp, 2000bp sequences in mouse α2(Ⅰ) [WTXFZ]procollagen gene 5'flanking region were studied with CAT as reporter gene and gene transfection method. ......
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