TUNEL技术原位观察肝细胞凋亡
肝肿瘤,病理学;脱噬作用;组织细胞学制备技术,项目负责人,Correspondenceto,Apoptosisinhumanhepatocellularcarcinomabyterminaldeoxynucleotidyltransferase-mediate,dUTP-FITCnickendlabeling,,Abstract,,AIM,METHODS,RESULTS,CONCLUSION
第四军医大学病理学教研室 陕西省西安市 710033李江，男，1972-05-28生，天津市人，汉族. 1995年第四军医大学本科毕业，1995年第四军医大学病理学硕士研究生，1997年第四军医大学博士研究生，发表论文5篇.
项目负责人 李江，710033，第四军医大学病理学教研室，陕西省西安市长乐西路17号.
Correspondence to Jiang Li, Department of Pathology, Cancer Research Institute, Fourth Military Medical University,Xi＇an 710033, Shannxi Province, China
Tel. +86·29·3287001
收稿日期 1997-12-30 接收日期 1998-02-25
Apoptosis in human hepatocellular carcinoma by terminal deoxynucleotidyl transferase-mediate
dUTP-FITC nick end labeling
Jiang Li, Wen-Liang Wang, Wen-Yong Wang, Bin Liu and Bo-Yun Wang
Department of Pathology, Cancer Research Institute, Fourth Military Medical University, Xi＇an 710033, Shaanxi Province, China
Abstract
AIM To examine the occurrence of apoptotic cell death in HCC-9204 cell line and 21 formalin fixed, paraffin-embedded biopsy specimens from liver cirrhosis (6 cases) and hepatocellular carcinoma (15 cases).
METHODS Apoptosis of HCC-9204 cell line was induced by adriamycin (20μmol/L). Modified TUNEL assay was conducted using in situ cell death detection kit fluorescein (Boehringer Mannheim). Sections were pretreated by microwave. Control experiments were also performed, including DNase-digestion before TUNEL as positive control and TdT and FITC-dUTP deletion as negative control. Routine HE staining was conducted on serial sections. ......
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