新霉素抑制促胃液素促人结肠癌细胞株SW480增殖的研究
新霉素;胃泌素;结肠肿瘤;细胞株SW480,项目负责人,Correspondenceto,Inhibitionofneomycinongastrin-stimulatingcellproliferationof,humancoloncelllineSW480,Abstract,AIM,METHODS,RESULTS,CONCLUSION,Subjectheadings
第三军医大学大坪医院野战外科研究所普通外科 重庆市 400042谢斌，男，1968-01-24生，四川省彭州市人，汉族. 1991年重庆医科大学医学系本科毕业，1998年第三军医大学普通外科硕士，医师，主要从事胃肠肿瘤的研究.
项目负责人 谢斌，400042，第三军医大学大坪医院野战外科研究所普通外科，重庆市长江支路10号.
Correspondence to Bin Xie, Department of General Surgery, Research Institute of Field Surgery, Daping Hospital, Third Military Medical University, 10 Changjiang Zhilu, Chongqing 400042, China
Tel. +86·23·68757247
收稿日期 1998-11-09
Inhibition of neomycin on gastrin-stimulating cell proliferation of human colon cell line SW480
Bin Xie, Shuang-Wu He and Dai-Ke Wang
Department of General Surgery, Research Institute of Field Surgery, Daping Hospital,Third Military Medical University, Chongqing 400042, China
Abstract
AIM To study the effect of neomycin (NM) on penta gastrin (PG) -stimulating cell proliferation of human colon cell line SW480, so as to elucidate the possible role and clinical significance of the phosphoinositide signalling pathway in proliferation of SW480 cells.
METHODS MTT assay was used for viable cell counts, [3H]-myo-inositol labeling method for the analysis of IP3, fluorescence measurements for [Ca2+]i, incorporation of phosphate from [γ-32P]ATP to histone for the measurement of the PKC activity. ......
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