胰腺癌抗原SC6的分离及其特性分析
胰腺肿瘤;重组噬菌体抗体;SC6抗原,全军医药卫生科研基金课题，,No.b96D002,项目负责人,SupportedbyMedicalandHealthScientificFundofthePLA,China,No.b96D002.,,Correspondenceto,PurificationandcharacterizationofantigenSC6forpancreaticc
沈阳军区总医院医学实验科 辽宁省沈阳市 110015刘民培，男，1948-03-21生，四川省蓬溪县人，汉族. 1975年白求恩医科大学放射医学系毕业，1991～1993年北京军事医学科学院学习，主任医师，教授，主要从事肿瘤免疫学研究，发表论文80篇，获国家和省部级二等奖5项.
全军医药卫生科研基金课题，No.b96D002
项目负责人 刘民培，110015，辽宁省沈阳市沈河区文化路83号，沈阳军区总医院医学实验科.
Supported by Medical and Health Scientific Fund of the PLA, China, No.b96D002.
Correspondence to Dr. Min-Pei Liu, Department of Experimental Medicine, Beifang Hospital, No.83 Wenhua Road, Shenhe District, Shenyang 110015, Liaoning Province, China
Tel. +86·24·23051421
收稿日期 1999-04-15
Purification and characterization of antigen SC6 for pancreatic cancer
Min-Pei Liu, Jing-Chun Zhou, Xiao-Zhong Guo, Wei Chen, Bing Dai, Tian-Yi An and Shi-Ying Ma
Department of Experimental Medicine, Beifang Hospital, Shenyang 110015, Liaoning Province, China
Abstract
AIM To obtain a target antigen for planning recombinant phage antibody (RPA) of pancreatic cancer.
METHODS Purification of the antigen was performed by affinity chromatography on CNBr-sepharose 4B columns binding monoclonal antibody SC6 purified by protein A-sepharose 4B columns. Nature of the purified antigen was examined by SDS-PAGE, Western blotting, immunochemistry, automatic analyzer of amino-acid etc. ......
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