幽门螺杆菌尿素酶B基因的克隆、表达及免疫原性研究
闂佺ǹ绻戞繛濠囧极椤撶姵瀚氱€广儱绻掔粣锟�螺杆菌,幽门;尿素酶B亚单位;克隆;基因表达;免疫原性,项目负责人,Correspondenceto,Cloning,expressionandimmunog-enicityofHpUreBgene,Abstract,,AIM,METHODS,RESULTS,CONCLUSION,Subjectheadings,摘要,目的,方
1解放军455医院消化内科 上海市 200052
2中国科学院上海生物化学研究所 上海市 200031
李明峰,男,1966-01-16生,甘肃省张掖市人,汉族. 1989年毕业于第四军医大学六年制本科,1989 年第四军医大学消化学硕士,主治医师,发表论文18篇.
项目负责人 李明峰,200052,上海市淮海西路338号,解放军455医院消化内科.
Correspondence to Dr. Ming-Feng Li, Department of Gastroenterology, Chinese PLA 455 Hospital, 338 Huaihai Westen Road, Shanghai 200052, China
Tel. +86·21·62811146(H)
收稿日期 1999-04-15
Cloning, expression and immunog-enicity of Hp UreB gene
Ming-Feng Li1, Zhen Ling1, Ai-Ying Ma1, Jian-Hua Zhao1, Jian-Xin Sun2, Sheng-Zhi Yu1 and Xiang-Fu Wu2
Department of Gastroenterology, Chinese PLA 455 Hospital, Shanghai 200052, China
Abstract
AIM To construct a recombinant strain which could express antigen of UreB subunit from Helicobacter pylori.
METHODS The gene encoding the structural B subunit of H, pylori urease was amplified from H.pylori chromosomal DNA by PCR. The gene was inserted in the prokaryotic expression vector pET-22b(+), and then was transformed into the BL-21(DE3) E.coli strain, expressed UreB recombinant protein. ......
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