定量检测HpcagA基因内参标模板的构建及测序
幽门螺杆菌;细胞毒素相关蛋白基因A;聚合酶链反应;内参标模板;乳糖操纵基因,项目负责人,Correspondenceto,ConstructionandsequencingofaninternalstandardtemplateforquantitativePCRdetectionofcagA+Hp,,Abstract,AIM,METHODS,RESU
中国人民解放军第四军医大学全军基因诊断技术应用研究所 陕西省西安市 710033韩锋产,男,1963-05-08生,陕西省咸阳市人,汉族. 1985年延安大学医学系毕业,1990年西安医科大学生物化学硕士,1998年第四军医大学生物化学于分子生物学博士研究生,现在第四军医大学全军基因诊断技术应用研究所工作,主要从事基因诊断方面的研究,发表论文12篇.项目负责人 韩锋产,710033,陕西省西安市长乐西路17号,中国人民解放军第四军医大学全军基因诊断技术研究所.
Correspondence to Feng-Chan Han, Chinese PLA Institute of Genetic Diagnosis, Fourth Military Medical University, 17 Changle Xilu, Xi'an 710033, Shaanxi Province, China
Tel. +86-29-3285729
Email. Hanfc@263.net
收稿日期 1999-12-22 接收日期 2000-01-08
Construction and sequencing of an internal standard template for quantitative PCR detection of cagA+ Hp
Feng-Chan Han, Xiao-Jun Yan, Yu Hou, Cheng-Zhi Su, Le-Yi Xiao, Yan-Hai Guo, Da-Xiang Cui and Shan-Qu Li
Chinese PLA Institute of Gene Diagnosis, Fourth Military Medical University,Xi'an 710033, Shaanxi Province, China
Abstract
AIM To construct an internal standard template for quantitative PCR detection of cagA+ Hp.
METHODS Two pairs of oligonucleotide primers were designed and synthesized according to the sequences of cagA and lac operator. The primers were cagAp1 ......
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