共聚焦方法分析P16基因的表达及对人肝癌细胞系721生长的作用
肝细胞瘤;基因表达;基因疗法;基因，抑制，肿瘤;原位杂交;转染,项目负责人,Correspondenceto,EffectofP16geneexpressioningrowthofhumanhepaticcarcinomacellline7721withconfocalmicroscopicanalysis,Abstract,A
中国人民解放军第四军医大学唐都医院传染科 陕西省西安市 710038王九平，女，1961-10-25生，陕西省西安市人，汉族. 1985年西安第四军医大学毕业，传染科主治医师，讲师，硕士. 主要从事病毒性肝炎发病机制的研究,发表论文7篇.
项目负责人 王九平，710038，陕西省西安市，中国人民解放军第四军医大学唐都医院传染科.
Department of Infectious Disease, Tangdu Hospital, Fourth Military Medical University, Xi＇an 710038, Shaanxi Province, China Correspondence to Jiu-Ping Wang, Department of Infectious Disease, Tangdu Hospital, The Fourth Military Medical University, Xi＇an 710038, Shaanxi Province, China
Tel. 0086-29-3577752, Fax: 0086-29-3373761
Email. wangxj@ fmmu.edu.cn
收稿日期 2000-05-05 接收日期 2000-05-11
Effect of P16 gene expression in growth of human hepatic carcinoma cell line 7721 with confocal microscopic analysis
Jiu-Ping Wang, Guo-Rong Duan, Yu-Ling Zhao and De-Wei Du
Abstract
AIM To explore the effect of P16 gene on the growth inhibition of hepatic carcinoma cell line 7721, Pathogenesis of hepatic carcinoma so as to offer theoretical basis for gene therapy.
METHODS Using subclone technic, the wild type P16 gene and irrelative DNA sequence were cloned into an eukaryotic express vector pcDNA3. Using Lipofectamine 2000, the recombinate plasmid pcDNA3-p16 was transfected into the 7721 human hepatic carcinoma cell line. mRNA of P16 in the 7721 cell was detected with in situ hybridization. Confocal microscopy was used to analyze P16 gene protein expression. Growth state of transfected 7721 cell was examined by MTT and FCM. ......
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