细胞凋亡——丝裂霉素致人肝细胞损伤机制的研究
细胞凋亡;肝,损伤;肝细胞;药物,毒性;体外研究;丝裂霉素;流式细胞术;抗肿瘤药,项目负责人,Correspondenceto,Apoptosis:astudyonmechanismofinjuriesinhumanhepatocyteinducedbymitomycin,,Abstract,AIM,METHODS,RESULT
湖北医科大学附属第二医院普外科 湖北省武汉市 430071陈绪军，男，1975-11-08生，湖北省武汉市人，汉族. 1996年6月湖北医科大学临床医学系本科毕业，学士，1999年于湖北医科大学获外科学硕士学位，住院医师. 主要从事肝、胆、胰疾病的诊治研究，已发表论文2篇，综述4篇.
项目负责人 陈绪军，430071，湖北省武汉市武昌区东湖路169号，湖北医科大学附属第二医院普外科.
Department of General Surgery, The Second Hospital Affiliated to Hubei Medical University, Wuhan 430071, Hubei Province, China
Correspondence to Xu-Jun Chen, Department of General Surgery, The Second Hospital Affiliated to Hubei Medical University, Wuhan 430071, Hubei Province, China
Tel. 0086-027-87317906
收稿日期 2000-03-08 接收日期 2000-04-15
Apoptosis: a study on mechanism of injuries in human hepatocyte induced by mitomycin
Xu-Jun Chen, Zhong-Li Ai and Zhi-Shu Liu
Abstract
AIM To investigate whether apoptosis can be induced by mitomycin in primarily cultured human hepatocyte.
METHODS Primarily cultured human hepatocytes were exposed to mitomycin. Apoptosis was evaluated by fluorescent stain, terminal deoxynucleotidyl transferase-mediated-dUTP-nick-end-labeling (TUNEL), agarose gel electrophoresis, transmission electron microscopy and flow cytometry (FCM). ......
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