华中科技大学同济医学院附属同济医院普外科 湖北省武汉市 430030

    王剑明，男，1968-11-11生，湖北省黄石市人，汉族. 1992年武汉同济医科大学毕业，1995年同济医院外科硕士，1997年同济医院肝胆外科博士，现为武汉同济医院主治医师，主要从事阻塞性黄疸基础与临床研究，发表论文11篇.

    项目负责人 王剑明, 430030, 湖北省武汉市汉口解放大道1095号, 华中科技大学同济医学院附属同济医院普外科. wjm18jgm@public.wh.hb.cn

    Tel: 0086-27-83621590,Fax: 0086-27-83692774

    收稿日期 2001-04-08 接受日期 2001-06-15

    Bcl-2 and Bax expressions and apoptosis in rat liver with obstructive jaundice

    Jian-Ming Wang, Qian Zou and Sheng-Quan ZouDepartment of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China

    Correspondence to Jian-Ming Wang, Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. wjm18jgm@public.wh.hb.cn

    Tel: 0086-27-83621590,Fax: 0086-27-83692774

    Received 2001-04-08 Accepted 2001-06-15

    

    Abstract

    AIM To explore the regulating mechanism of hepatic injury in obstructive jaundice (OJ).

    

    METHODS Forty adult male Wistar rats (190g-230g) were used for all experiments. All rats underwent laparotomy. Experimental obstructiv e jaundice (BDL) was induced by double ligation of the bile duct by a proximal ligature around the bile duct in the hilus of the liver and by a distal ligature close to its entry into the duodenum. The animals were randomly divided into two groups: those that underwent bile duct manipulation (sham operated) and those that underwent BDL for 3, 7, 14 and 21 days. We detected the bilirubin of blood and pathological changes of liver in alle rats. Then we observed the apoptotic status and the expression of apoptotic related gene Bcl-2, Bax protein in liver of alle rats by the terminal-deoxynucleotidyl mediated nick end labeling (TUNEL) and immunohistochemistry method. ......