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乙型肝炎病毒X基因酵母表达载体构建及表达
http://www.100md.com 2002年1月15日 《世界华人消化杂志》 2002年第1期
项目负责人,CloningandexpressionofhepatitisBvirusXgeneinyeast,Correspondenceto:,Abstract,AIM:,METHODS:,RESULTS:,CONCLUSIONS:,摘要,目的:,方法:,结果:,结论:,0引言,1材料和方法,1.1材料,1.2方法,1.2.1HBxAg基因的扩增,1.2.
     陆荫英,李克,成军,王琳,刘妍,段惠娟,张玲霞,中国人民解放军第302医院传染病研究所基因治疗研究中心 北京市 100039

    陆荫英,1974-08-27,女,贵州省贵阳人,2000年军医进修学院传染病学专业博士研究生,发表论文3篇.

    项目负责人 成军,100039,北京市,丰台区丰台路26号基因治疗研究中心. cj@genetherapy.com.cn

    电话: 010-66933392

    收稿日期 2001-10-21 接受日期 2001-11-01

    Cloning and expression of hepatitis B virus X gene in yeast

    
Yin-Ying Lu, Ke Li, Jun Cheng, Lin Wang, Yan Liu, Hui-juan Duan,Ling-Xia Zhang

    Yin-Ying Lu, Ke Li, Jun Cheng, Lin Wang, Yan Liu, Hui-juan Duan,Ling-Xia Zhang.Gene Therapy Research Center, Institution of Infectious Diseases, Chinese PLA 302 Hospital, 100039, Beijing, China

    Correspondence to: Yin-Ying Lu, Gene Therapy Research Center, Institution of Infectious Diseases, PLA 302 Hospital, Luyinying 100039, Beijing, China. luyinying@263.net

    Received 2001-10-21 Accepted 2001-11-01

    

    Abstract
AIM:To study the exact function of HBx protein so as to investigate the gene expression of HBxAg in yeast.

    METHODS:PCR was used to amplify the gene of HBx from the plasmid pCP10 containing the whole fragment of ayw subtype of HBV and the PCR product was cloned into pGEM-T vector and was evaluated by sequencing. The gene of HBx was cut from pGEM-T vector and cloned into yeast expression plasmid pGBKT7, and was transformed into yeast cell AH109. The yeast protein was isolated, and analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. ......

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