肝靶向基因给药系统AsOR-PL+ PEG-PEI的研究
肝靶向基因给药系统AsOR-PL+PEG-PEI的研究,项目负责人,Multi-componentDNAdeliverysystemtargetedforhepatocyte-AsOR-PL,PEG-PEI,Correspondenceto:,Abstract,AIM:,METHODS:,RESULTS:,CONCLUSION:,摘要,目的:,方法:,结果:,结论:,0引言,1材料和方法,1.1材料,1.2方法
靳雪源，张玲霞，楼敏，谢建芳，中国人民解放军第302医院感染五科 北京市 100039靳雪源，女，1966-07-23生，河北省三河市人，汉族，1989年山西医科大学(原山西医学院)本科毕业，主治医师，从事肝病临床及实验研究.
项目负责人 靳雪源，100039，北京市丰台区丰台路26号，中国人民解放军第302医院感染五科. jinxueyuan@Yahoo.com
电话: 010-66933129-6411 传真：010-63801283
收稿日期 2001-12-21 接受日期 2002-01-04
Multi-component DNA delivery system targeted for hepatocyte-As OR-PL/PEG-PEI
Xue-Yuan Jin, Ling-Xia Zhang, Min Lou, Jian-Fang Xie
Xue-Yuan Jin, Ling-Xia Zhang, Min Lou, Jian-Fang Xie, the 5th Division of the 302th Hospital of PLA, Beijing 100039,China
Correspondence to: Xue-Yuan Jin, the 5th Division of the 302th Hospital of PLA,Beijing 100039,China. jinxueyuan@Yahoo.com
Received 2001-12-21 Accepted 2002-01-04
Abstract
AIM:To develop a multi-component DNA delivery system which incorporated hepatocyte targeting ligand, DNA compressing domain and endosome disruptive molecule in one complex.
METHODS:AsOR was conjugated to PL by EDC; The active ester of PEG derivative was reacted with the amino groups in PEI to form PEG-PEI conjugate; DNA was first complexed with PEG-PEI and then with AsOR-PL. In vitro transfection experiment was conducted in the galactose receptor positive Huh-7 cells. In vivo transduction was also evaluated in mice after tail vein injection. ......
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