甘氨鹅脱氧胆酸钠致阻塞性黄疸大鼠肝细胞凋亡中 bcl-2 mRNA的表达意义
甘氨鹅脱氧胆酸钠致阻塞性黄疸大鼠肝细胞凋亡中bcl-2mRNA的表达意义,项目负责人,Expressionofbcl-2mRNAinhepatocyticapoptosisinducedbyGCDCinratobstructivejaundicebyRT-PCR,Correspondenceto:,Abstract,AIM:,METHODS:,RESULTS:,CONCLUSION:,摘要,目的:,方法:,结果:
王剑明，邹声泉，华中科技大学同济医学院附属同济医院普外科 湖北省武汉市 430030王剑明，男，1968-11-11生，湖北黄石市人，汉族,肝胆外科博士，主治医师.
项目负责人 王剑明，430030，武汉汉口解放大道1095号，华中科技大学同济医学院附属同济医院普外科.
收稿日期 2001-12-20 接受日期 2002-01-07
Expression of bcl-2 mRNA in hepatocytic apoptosis induced by GCDC in rat obstructive jaundice by RT-PCR
Jian-Ming Wang, Sheng-Quan Zou
Jian-Ming Wang, Sheng-Quan Zou，Department of General Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430032,China
Correspondence to: Jian-Ming Wang, Departmentof Surgery,Tongji Hospital, Tongji Medical College,Huazhong Science and Technology, Wuhan 430030,Hubei Province,China. wjm18jgm@public.wh.hb.cn
Received 2001-12-20 Accepted 2002-01-07
Abstract
AIM:To explore the regulating mechanism of bcl-2 gene in liver cell apoptosis in experimental obstructive jaundice.
METHODS:The peritoneal cavity of male Wistar rats was opened, and the common bile duct was double ligated and cut between the ligatures. Controls underwent a sham operation that was consisted of exposure, but not ligation, of the common bile duct. Experimental obstructive jaundice (BDL) was induced by double ligation of the bile duct at 3, 7, 14 and 21 days after operation. Every group consisted of 8 rats. In normal and experimental rats after 7, 14, 21d, hepatocytes were isolated by in situ collagenase perfusion and primary culture was set up. Total cellular RNA was isolated using the standard acid guanidinium thiocyanate-phenolchloroform extraction method and further purified by cesiumchloride gradient centrifugation. bcl-2 mRNA was detected by RT-PCR in all the cells. After added with 100μmol·L-1 GCDC for 24h, the cells were evaluated by FCM and TUNEL. ......
您现在查看是摘要页，全文长 19366 字符。