王玉梅, 冯国和, 赵桂珍, 乔光彦，中国医科大学第二临床学院传染科 辽宁省沈阳市 110004

    王玉梅, 女，1970-02-26生，山西省大同市人，1993年沈阳中国医科大学本科毕业，1996年中国医科大学硕士研究生毕业，1999年开始于中国医科大学攻读博士学位，讲师，主要从事病毒性肝炎发病机制的研究.

    项目负责人 王玉梅,110004，辽宁省沈阳市，中国医科大学第二临床学院传染科

    电话: 024-23893501-6981

    收稿日期 2002-03-05 接受日期 2002-03-14

    The roles of tumor necrosis factor-αand nitric oxide on liver injury in fulminant hepatic failure

    Yu-Mei Wang,Guo-He Feng,Gui-Zhen Zhao,Guang-Yan Qiao

    Yu-Mei Wang,Guo-He Feng,Gui-Zhen Zhao,Guang-Yan Qiao，Department of infectious disease,2nd clinical college of China Medical University,Shenyang,Liaoning Province,China,110004

    Correspondence to: Yu-Mei Wang， Department of infectious disease,2nd clinical college of China Medical University,Shenyang,Liaoning Province,China,110004

    Received 2002-03-05 Accepted 2002-03-14

    Abstract

    AIM:To study the roles of tumor necrosis factor-α(TNF-α)and nitric oxide(NO) on liver injury and the mural effect of the two factors in fulminant hepatic failure(FHF).

    METHODS:We established experimental model of FHF by LPS and D-GalN. Serumal NO level and iNOSmRNA expression in liver were studied by biochemistry method and RT-PCR method respectively, while serumal TNF-αlevel and TNF-α mRNA expression in liver were tested by ELISA method and RT-PCR method respectively. The change of TNF-α,NO and liver injury were observed in the different phase after drug administration. In addition, we observed the change of above items after pretreatment with anti-TNF-α IgG1 and NO synthase inhibitor L-NMMA respectively. ......