袁志强，李晓鲁，彭毅志，中国人民解放军第三军医大学西南医院烧伤研究所 重庆市 400038

    袁志强，男，1976-3-18生，湖南省汝城县人，汉族.1997年第三军医大学毕业，现为第三军医大学烧伤外科学博士研究生，主要从事脏器缺血再灌注损伤及感染防治的研究，发表论文8篇.

    国家重点基础研究发展规划项目，(G1999054202)

    项目负责人 彭毅志，400038，重庆市高滩岩，第三军医大学西南医院烧伤研究所. cqmed@sina.com

    电话： 023-68754175

    收稿日期 2002-03-05 接受日期 2002-03-22

    Effect of inducible HSP70 on hypoxia/ reoxygenation induced injury of intestinal epithelial cell in vitro

    Zhi-Qiang Yuan, Xiao-Lu Li, Yi-Zhi Peng

    Zhi-Qiang Yuan, Xiao-Lu Li, Yi-Zhi Peng, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China

    Supported by major stat basic Research development program of China (G1999054202)

    Correspondence to: Dr Yi-Zhi Peng, Institute of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, China. cqmed@sina.com

    Received 2002-03-05 Accepted 2002-03-22

    AbstractAIM: To study the mechanism and effect of inducible heat shock protein 70 (HSP70) on hypoxia/reoxygenation induced injury of intestinal epithelial cell.

    METHODS: Cultured IEC-6 cells were divided into three groups: Control group (C), hypoxia/reoxygenation group (H/R) and heat shock pretreated group (HS+H/R). After 1 hour of hypoxia followed by 1 hour of reoxygenation, Immunohistochemical staining was used to determine the expression of HSP70 in IEC-6 cells. Cell viability was measured by MTT assay and lactate dehydrogenase leakage were analyzed, and the cell apoptosis was evaluated with TUNEL staining. ......