方肇勤,管冬元,梁尚华,上海中医药大学基础医学院 上海市 200032

    国家中医药管理局科研基金, NO.97Z014

    项目负责人:方肇勤,200032,上海市零陵路530号,上海中医药大学基础医学院. zqfang@sh163d.sta.net.cn

    电话:021-54231178

    收稿日期:2002-07-20 接受日期:2002-08-07

    Regulation of gene transcription in rat with liver cancer by traditional Chinese medicine

    Zhao-Qin Fang, Dong-Yuan Guan,Shang-Hua Liang

    Zhao-Qin Fang , Dong-Yuan Guan,Shang-Hua Liang, Institute of Basic Medicine, Shanghai University of TCM, Shanghai 200032,China

    Supported by National Traditional Chinese Medicine Administration Bureau. NO:97Z014

    Correspondence to:Professor Zhao-Qin Fang, Institute of Basic Medicine, Shanghai University of TCM, 530 Lingling Road, Shanghai 200032, China. zqfang@sh163d.sta.net.cn.

    Received:2002-07-20 Accepted:2002-08-07

    AbstractAIM:To observe the regulatory effects of YAF Compositus and its modified Compositus: Gingrefang, Huoxuefang and Jianpifang on liver cancer and their transcription the genes in rats.

    METHODS:DEN was used to induce the liver cancer in rats. All rats were divided into seven groups:normal group, model group, four therapy groups and FT-207 group. The survival rate, liver weight, body weight, liver weight/body weight, and pathological change of liver tissues were observed. Immunocytochemical change of AFP and DD-PCR was used to display the different transcriptional expression of cDNA fragments between normal liver tissues and DEN-induced liver cancer tissues. Northern blotting was used to verify the different transcription of these cDNA fragments. ......