乙型肝炎病毒X基因启动子结构及调节研究
邵清,成军,中国人民解放军第302医院传染病研究所基因治疗研究中心、全军病毒性肝炎防治研究重点实验室 北京市 100039
白雪帆,中国人民解放军第四军医大学唐都医院感染科 陕西省西安市 710038
国家自然科学基金项目,No. C03011402, No. C30070689
军队“九、五”科技攻关项目,No.98D063
军队回国留学人员启动基金项目,No.98H038
军队“十、五”科技攻关青年基金项目,No.01Q138
军队“十、五”科技攻关面上项目,No.01MB135
, 百拇医药
项目负责人:成军, 100039,北京市西四环中路100号,中国人民解放军第302医院传染病研究所基因治疗研究中心、全军病毒性肝炎防治研究重点实验室. cj@genetherapy.com.cn
电话:010-66933391 传真:010-63801283
收稿日期:2003-03-08 接受日期:2003-04-16
邵清,成军, 白雪帆.乙型肝炎病毒X基因启动子结构及调节研究.世界华人消化杂志 2003;11(8):1240-1242
Guo SP, Ma ZS, Wang WL. Construction of eukaryotic expressionvector of HBV x gene. World J Gastroenterol
, http://www.100md.com
1999;5:351-352
2 Tang RX, Gao FG, Zeng LY, Wang YW, Wang YL. Detection of HBV DNAand its existence status in liver tissues
andperipheral blood lymphocytes from chronic hepatitis B patients. World J Gastroenterol 1999;5:359-361
3 Qin LL, Su JJ, Li Y, Yang C, Ban KC, Yian RQ. Expression of IGF-II, p53, p21 and HBxAg in precancerous events
ofhepatocarcinogenesis induced by AFB1 and/or HBV in tree shrews. World JGastroenterol 2000;6:138-139
, http://www.100md.com
4 Wang Y, Liu H, Zhou Q, Li X. Analysis of point mutation in site1896 of HBV precore and its detection in the tissues
andserum of HCC patients. World J Gastroenterol 2000;6:395-397
5 骆抗先,朱幼芙. 乙型肝炎基础和临床.第2版.北京: 人民卫生出版社, 2001:25-33
6 Zheng YW, Riegler J, Wu J, Yen TS. Novel short transcripts ofhepatitis B virus X gene derived from intragenic promoter.
JBiol Chem 1994;269:22593-22598
, 百拇医药
7 Zhang P, Raney AK, McLachlan A. Characterization of the hepatitis Bvirus X-and nucleocapsid gene
transcriptionalregulatory elements. Virology 1992;191:31-41
8 Treinin M, Laub O. Identification of a promoter element locatedupstream from the hepatitis B virus X gene. Mol Cell
Biol 1987;7:545-548
9 Fukai K, Takada S, Yokosuka O, Saisho H, Omata M, Koike K.Characterization of a specific region in the hepatitis B
, http://www.100md.com
virusenhancer I for the efficient expression of X gene in the hepatic cell. Virology 1997;236:279-287
10 Takada S, Kaneniwa N, Tsuchida N, Koike K. Hepatitis B virus X geneexpression is activated by X protein but repressed
byp53 tumor suppressor gene product in the transient expression system.Virology 1996;216:80-89
11 Nakamura I, Koike K. Identification of a binding protein to the Xgene promoter region of hepatitis B virus. Virology
, 百拇医药
1992; 191:533-540
12 Yaginuma K, Nakamura I, Takada S, Koike K. A transcriptioninitiation site for the hepatitis B virus X gene is directed by
thepromoter-binding protein. J Virol 1993;67:2559-2565
13 Guo WT, Bell KD, Ou JH. Characterization of the hepatitis B virusEnhI enhancer and X promoter complex. J
Virol 1991;65:6686-6692
, 百拇医药
14 Gustin K, Shapiro M, Lee W, Burk RD. Characterization of the roleof individual protein binding motifs within the hepatitis
Bvirus enhancer I on X promoter activity using linker scanning mutagenesis.Virology 1993;193:653-660
15 Xu ZH, Zhao MJ, Li TP. p73beta inhibits transcriptional activitiesof enhancer I and X promoter in hepatitis B virus
moreefficiently than p73alpha. World J Gastroenterol 2002;8:1094-1097
, 百拇医药
16 Choi CY, Choi BH, Park GT, Rho HM. Activating transcription factor2 (ATF2) down-regulates hepatitis B virus X
promoter activity by the competitionfor the activating protein 1 binding site and the formation of the ATF2-
Junheterodimer. J Biol Chem 1997;272:16934-16939
17 Chol CY, Park GT, Rho HM. A positive regulatory sequence ofhepatitis B viral small X promoter. Eur J
Biochem 1996;239:579-587, http://www.100md.com( 邵 清, 成 军, 白雪帆)
白雪帆,中国人民解放军第四军医大学唐都医院感染科 陕西省西安市 710038
国家自然科学基金项目,No. C03011402, No. C30070689
军队“九、五”科技攻关项目,No.98D063
军队回国留学人员启动基金项目,No.98H038
军队“十、五”科技攻关青年基金项目,No.01Q138
军队“十、五”科技攻关面上项目,No.01MB135
, 百拇医药
项目负责人:成军, 100039,北京市西四环中路100号,中国人民解放军第302医院传染病研究所基因治疗研究中心、全军病毒性肝炎防治研究重点实验室. cj@genetherapy.com.cn
电话:010-66933391 传真:010-63801283
收稿日期:2003-03-08 接受日期:2003-04-16
邵清,成军, 白雪帆.乙型肝炎病毒X基因启动子结构及调节研究.世界华人消化杂志 2003;11(8):1240-1242
Guo SP, Ma ZS, Wang WL. Construction of eukaryotic expressionvector of HBV x gene. World J Gastroenterol
, http://www.100md.com
1999;5:351-352
2 Tang RX, Gao FG, Zeng LY, Wang YW, Wang YL. Detection of HBV DNAand its existence status in liver tissues
andperipheral blood lymphocytes from chronic hepatitis B patients. World J Gastroenterol 1999;5:359-361
3 Qin LL, Su JJ, Li Y, Yang C, Ban KC, Yian RQ. Expression of IGF-II, p53, p21 and HBxAg in precancerous events
ofhepatocarcinogenesis induced by AFB1 and/or HBV in tree shrews. World JGastroenterol 2000;6:138-139
, http://www.100md.com
4 Wang Y, Liu H, Zhou Q, Li X. Analysis of point mutation in site1896 of HBV precore and its detection in the tissues
andserum of HCC patients. World J Gastroenterol 2000;6:395-397
5 骆抗先,朱幼芙. 乙型肝炎基础和临床.第2版.北京: 人民卫生出版社, 2001:25-33
6 Zheng YW, Riegler J, Wu J, Yen TS. Novel short transcripts ofhepatitis B virus X gene derived from intragenic promoter.
JBiol Chem 1994;269:22593-22598
, 百拇医药
7 Zhang P, Raney AK, McLachlan A. Characterization of the hepatitis Bvirus X-and nucleocapsid gene
transcriptionalregulatory elements. Virology 1992;191:31-41
8 Treinin M, Laub O. Identification of a promoter element locatedupstream from the hepatitis B virus X gene. Mol Cell
Biol 1987;7:545-548
9 Fukai K, Takada S, Yokosuka O, Saisho H, Omata M, Koike K.Characterization of a specific region in the hepatitis B
, http://www.100md.com
virusenhancer I for the efficient expression of X gene in the hepatic cell. Virology 1997;236:279-287
10 Takada S, Kaneniwa N, Tsuchida N, Koike K. Hepatitis B virus X geneexpression is activated by X protein but repressed
byp53 tumor suppressor gene product in the transient expression system.Virology 1996;216:80-89
11 Nakamura I, Koike K. Identification of a binding protein to the Xgene promoter region of hepatitis B virus. Virology
, 百拇医药
1992; 191:533-540
12 Yaginuma K, Nakamura I, Takada S, Koike K. A transcriptioninitiation site for the hepatitis B virus X gene is directed by
thepromoter-binding protein. J Virol 1993;67:2559-2565
13 Guo WT, Bell KD, Ou JH. Characterization of the hepatitis B virusEnhI enhancer and X promoter complex. J
Virol 1991;65:6686-6692
, 百拇医药
14 Gustin K, Shapiro M, Lee W, Burk RD. Characterization of the roleof individual protein binding motifs within the hepatitis
Bvirus enhancer I on X promoter activity using linker scanning mutagenesis.Virology 1993;193:653-660
15 Xu ZH, Zhao MJ, Li TP. p73beta inhibits transcriptional activitiesof enhancer I and X promoter in hepatitis B virus
moreefficiently than p73alpha. World J Gastroenterol 2002;8:1094-1097
, 百拇医药
16 Choi CY, Choi BH, Park GT, Rho HM. Activating transcription factor2 (ATF2) down-regulates hepatitis B virus X
promoter activity by the competitionfor the activating protein 1 binding site and the formation of the ATF2-
Junheterodimer. J Biol Chem 1997;272:16934-16939
17 Chol CY, Park GT, Rho HM. A positive regulatory sequence ofhepatitis B viral small X promoter. Eur J
Biochem 1996;239:579-587, http://www.100md.com( 邵 清, 成 军, 白雪帆)
