paxillin在胃腺癌中的表达及临床意义
田素芳,熊永炎, 余少平,汪必成,武汉大学中南医院病理科 湖北省武汉市 430071
项目负责人:熊永炎,430071, 湖北省武汉市东湖路169号,武汉大学中南医院病理科.
电话:027-67813159
收稿日期:2002-11-12 接受日期:2002-11-29
摘要
目的:研究焦点黏附相关调节蛋白paxillin在胃癌组织中的表达及其与胃癌细胞分化、浸润及转移的关系.
, http://www.100md.com 方法:应用免疫组化SP法检测80例胃癌和40例淋巴结转移灶中paxillin的表达情况.
结果:paxillin在胃中、低分化腺癌组织中的表达显著高于高分化腺癌，进展期胃癌组织中显著高于早期胃癌，胃癌原发灶中显著高于淋巴结转移灶.
结论:paxillin可能在胃癌细胞的分化、浸润和转移中起着重要作用.
田素芳,熊永炎, 余少平,汪必成.paxillin在胃腺癌中的表达及临床意义.世界华人消化杂志 2003;11(10):1632-1634
Clark EA, Brugge JS. Integrins and signal transduction pathways:theroad taken. Science 1995;268:233-239
, 百拇医药
2 Schaller MD. Paxillin: a focal adhesion-associated adaptorprotein.Oncogene 2001;20:6459-6472
3 Iwasaki T, Nakata A, Mukai M, Shinkai K, Yano H, Sabe H, SchaeferE, Tatsuta M, Tsujimura T, Terada N, Kakishita
E, Akedo H. Involvement of phosphorylationof Tyr-31 and Tyr-118 of paxillin in MM1 cancer cell migration. Int JCancer
2002;97:330-335
4 Shigetomi A, Kotoh T, Harada T, Morikawa S, Nakamura T. Developmentof an experimental model for spontaneous
, 百拇医药
lymph node metastasis of human esophagealcarcinoma in nude mice histopatholegicol analysis. Hum Cell
1992;5:273-281
5 Jockusch BM, Bubeck P, Giehl K, Kroemker M, Moschner J, RothkegelM, Rudiger M, Schluter K, Stanke G, Winkler J.
The molecular architecture of focaladhesions. Annu Rev Cell Dev Biol 1995;11:376-416
6 Tu LC, Chou CK, Chen HC, Yeh SF. Protein kinase C-mediated tyrosinephosphorylation of paxillin and focal adhesion
, 百拇医药
kinase requires cytoskeletal integrity andis uncoupled to mitogen-activated protein kinase activation in human
hepatoma cells. J Biomed Sci 2001;8:184-190
7 Haier J, Nicolson GL. Role of the cytoskeleton in adhesionstabilization of human colorectal carcinoma cells to
extracellular matrix components underdynamic conditions of laminar flow. Clin Exp Metastasis 1999;17:713-721
, 百拇医药
8 Turner CE. Paxillin interactions. J Cell Sci 2000;113:4139-4140
9 Vadlamudi R, Adam L, Tseng B, Costa L, Kumar R. Transcripitionalup-regulation of paxillin expression by heregulin
in human breast cancer cells. Cancer Res 1999;59:2843-2846
10 Salgia R, Li JL, Ewaniuk DS, Wang YB, Sattler M, Chen WC, RichardsW, Pisick E, Shapiro GI, Rollins BJ, Chen LB, Griffin
, 百拇医药
JD, Sugarbaker DJ. Expression of the focaladhesion protein paxillin in lung cancer and its relation to cellmotility.
Oncogene 1999;18:67-77
11 刘红岩.整合素激活FAK介导的信号传导途径研究进展.国外医学免疫学分册 2000;23:1-5
12 Rodina A, Schramm K, Musatkina E, Kreuser ED, Tavitian A, TatosyanA. Phosphorylation of p125FAK and paxillin
focal adhesion proteins in src-transformedcells with different metastatic capacity. FEBS Lett 1999;455:145-148
, http://www.100md.com
13 Lu Z, Jiang G, Blume-Jensen P, Hunter T. Epidermal growthfactor-induced tumor cell invasion and metastasis initiated
by dephosphorylation and downregulation offocal adhesion kinase. Mol Cell Biol 2001;21:4016-4031
14 苏剑敏,桂律, 周逸平,查锡良. 粘着斑激酶在胃癌中的表达及其临床意义.实用癌症杂志 2000;15:341-343
15 Cajot JF, Sordat I, Silvestre T, Sordat B. Differential displaycloning identifies motility 杛elatedprotein (MRP1/CD9)
as highly expressed in primary compared tometastatic human colon carcinoma cells. Cancer Res 1997;57:2593-2597, http://www.100md.com( 田素芳, 熊永炎, 余少平, 汪必成)
项目负责人:熊永炎,430071, 湖北省武汉市东湖路169号,武汉大学中南医院病理科.
电话:027-67813159
收稿日期:2002-11-12 接受日期:2002-11-29
摘要
目的:研究焦点黏附相关调节蛋白paxillin在胃癌组织中的表达及其与胃癌细胞分化、浸润及转移的关系.
, http://www.100md.com 方法:应用免疫组化SP法检测80例胃癌和40例淋巴结转移灶中paxillin的表达情况.
结果:paxillin在胃中、低分化腺癌组织中的表达显著高于高分化腺癌，进展期胃癌组织中显著高于早期胃癌，胃癌原发灶中显著高于淋巴结转移灶.
结论:paxillin可能在胃癌细胞的分化、浸润和转移中起着重要作用.
田素芳,熊永炎, 余少平,汪必成.paxillin在胃腺癌中的表达及临床意义.世界华人消化杂志 2003;11(10):1632-1634
Clark EA, Brugge JS. Integrins and signal transduction pathways:theroad taken. Science 1995;268:233-239
, 百拇医药
2 Schaller MD. Paxillin: a focal adhesion-associated adaptorprotein.Oncogene 2001;20:6459-6472
3 Iwasaki T, Nakata A, Mukai M, Shinkai K, Yano H, Sabe H, SchaeferE, Tatsuta M, Tsujimura T, Terada N, Kakishita
E, Akedo H. Involvement of phosphorylationof Tyr-31 and Tyr-118 of paxillin in MM1 cancer cell migration. Int JCancer
2002;97:330-335
4 Shigetomi A, Kotoh T, Harada T, Morikawa S, Nakamura T. Developmentof an experimental model for spontaneous
, 百拇医药
lymph node metastasis of human esophagealcarcinoma in nude mice histopatholegicol analysis. Hum Cell
1992;5:273-281
5 Jockusch BM, Bubeck P, Giehl K, Kroemker M, Moschner J, RothkegelM, Rudiger M, Schluter K, Stanke G, Winkler J.
The molecular architecture of focaladhesions. Annu Rev Cell Dev Biol 1995;11:376-416
6 Tu LC, Chou CK, Chen HC, Yeh SF. Protein kinase C-mediated tyrosinephosphorylation of paxillin and focal adhesion
, 百拇医药
kinase requires cytoskeletal integrity andis uncoupled to mitogen-activated protein kinase activation in human
hepatoma cells. J Biomed Sci 2001;8:184-190
7 Haier J, Nicolson GL. Role of the cytoskeleton in adhesionstabilization of human colorectal carcinoma cells to
extracellular matrix components underdynamic conditions of laminar flow. Clin Exp Metastasis 1999;17:713-721
, 百拇医药
8 Turner CE. Paxillin interactions. J Cell Sci 2000;113:4139-4140
9 Vadlamudi R, Adam L, Tseng B, Costa L, Kumar R. Transcripitionalup-regulation of paxillin expression by heregulin
in human breast cancer cells. Cancer Res 1999;59:2843-2846
10 Salgia R, Li JL, Ewaniuk DS, Wang YB, Sattler M, Chen WC, RichardsW, Pisick E, Shapiro GI, Rollins BJ, Chen LB, Griffin
, 百拇医药
JD, Sugarbaker DJ. Expression of the focaladhesion protein paxillin in lung cancer and its relation to cellmotility.
Oncogene 1999;18:67-77
11 刘红岩.整合素激活FAK介导的信号传导途径研究进展.国外医学免疫学分册 2000;23:1-5
12 Rodina A, Schramm K, Musatkina E, Kreuser ED, Tavitian A, TatosyanA. Phosphorylation of p125FAK and paxillin
focal adhesion proteins in src-transformedcells with different metastatic capacity. FEBS Lett 1999;455:145-148
, http://www.100md.com
13 Lu Z, Jiang G, Blume-Jensen P, Hunter T. Epidermal growthfactor-induced tumor cell invasion and metastasis initiated
by dephosphorylation and downregulation offocal adhesion kinase. Mol Cell Biol 2001;21:4016-4031
14 苏剑敏,桂律, 周逸平,查锡良. 粘着斑激酶在胃癌中的表达及其临床意义.实用癌症杂志 2000;15:341-343
15 Cajot JF, Sordat I, Silvestre T, Sordat B. Differential displaycloning identifies motility 杛elatedprotein (MRP1/CD9)
as highly expressed in primary compared tometastatic human colon carcinoma cells. Cancer Res 1997;57:2593-2597, http://www.100md.com( 田素芳, 熊永炎, 余少平, 汪必成)
