彭丽华,杨云生, 孙刚,王巍峰,中国人民解放军总医院消化内科北京市 100853

    彭丽华,女, 1972年生,北京市人, 汉族,学士, 主治医师,主要从事消化系疾病的临床工作和胃肠功能性及动力疾病的研究.

    项目负责人:杨云生,100853, 北京市复兴路28号,中国人民解放军总医院消化内科. sunny888@medmail.com.cn

    电话:010-66939747

    收稿日期:2003-06-21 接受日期:2003-08-18

    Anew model of constipation-predominant irritable bowel syndrome in rats

    Li-HuaPeng, Yun-Sheng Yang, Gang Sun, Wei-Feng Wang

    Li-HuaPeng, Yun-Sheng Yang, Gang Sun, Wei-Feng Wang, General Hospital of ChinesePLA, Beijing 100853, China

    Correspondence to: Yun-Sheng Yang, Department of Gastroenterology,General Hospital of Chinese PLA, Beijing 100853, China. sunny888@medmail.com.cn

    Received:2003-06-21 Accepted:2003-08-18

    AbstractAIM: To set up a new model of constipation-predominant irritable bowelsyndrome (C-IBS) in rats and to study the possible pathogenesis of IBS.

    METHODS:Wistar rats of postnatal four weeks were divided into three groups: groupA (stomach irritation with 0-4 °C cool water), groupB (stomach irritation with room temperature water) and group C (controlgroup). Both of rats in groups A and B received stomach irritation dailyfor fourteen days. In these fourteen days, the feces of rats in threegroups were collected and the feces granules were counted, moisturecontents of the feces were calculated daily during 0 to 3 hours and 3 to24 hours, respectively. After suspending the irritation of cool water, thegranules and moisture contents of feces in corresponding periods wereobserved for the following fourteen days continuously in order to evaluatethe degree of constipation. Then perception thresholds and the number ofabdominal withdrawal reflexes (AWR) were recorded during rectal balloondistention in order to evaluate bowel sensitivity. The ileocecal junctionand colon samples were stained by haematoxylin-eosin (HE) for routinepathologic examination and the improved toluidine blue method for mastcells, respectively. The expression of 5-hydroxymetryptamine (5-HT) onbowel was showed by immunohistochemical staining, and analyzedsemi-quantitatively by computer color image analyzer. ......