SV40T外显子的拼接及逆转录病毒载体pLLTSN的构建
李俊刚,陈耀凯,王宇明,项目负责人,:,SplicingofSV40Tgeneexonsandconstructionofaretroviralvector,pLLTSN,Supportedby,Correspondenceto:,Abstract,摘要,目的,:,方法,结果,结论,0,引言,1,材料和方法,1.1材料,1.2,2,结果,2.1
李俊刚,陈耀凯, 王宇明,中国人民解放军第三军医大学西南医院全军感染病研究所 重庆市 400038李俊刚,男, 1975-06-10生,贵州省平坝县人,汉族. 2001年第三军医大学硕士生,主要从事生物人工肝细胞材料的研究.
国家自然科学基金资助项目,No. 30100080
全军医学“十五”计划面上项目,No.01MA177
项目负责人:陈耀凯,400038, 重庆市,中国人民解放军第三军医大学西南医院全军感染病研究所. yaokaichen@hotmail.com
电话:023-68754475-8006 传真:023-65397072
收稿日期:2003-12-10 接受日期:2004-02-01
Splicing of SV40T gene exons and construction of a retroviral vectorpLLTSN
Jun-GangLi, Yao-Kai Chen, Yu-Ming Wang
Jun-Gang Li, Yao-Kai Chen, Yu-Ming Wang, Institute of Infectious Diseases,Southwest Hospital, Third Military Medical University, Chongqing 400038,China
Supported by the National Natural Science Foundation of China,No.30100080, and PLA Tenth Five-Year Medicine Project, No. 01MA177
Correspondence to: Yao-Kai Chen, Institute of Infectious Diseases,Southwest Hospital, Third Military Medical University, Chongqing 400038,China. yaokaichen@hotmail.com
Received: 2003-12-10 Accepted:2004-02-01
AbstractAIM: To construct an immortalization vector for hepatocytesimmortalization, and two exons of simian virus 40 large T antigen gene(SV40T) were spliced and a retroviral vector pLLTSN without intron wasconstructed. ......
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