HBV PreS2S/Fc融合基因真核表达载体的构建及表达
HBVPreS2S,Fc融合基因真核表达载体的构建及表达,陈红梅,白雪帆,黄长形,李光玉,洪沙,项目负责人,:,Constructionandexpressionof,eukaryoticvectorbearingfusiongeneofHBVPreS2SandFcfragment,Correspondenceto:,Abstract,摘要,目的,方法,结果,结论,0,引言,1,材料和方法,1.1材料
陈红梅,白雪帆, 黄长形,李光玉, 洪沙,中国人民解放军第四军医大学唐都医院感染科 陕西省西安市 710038陈红梅,女, 1975-06-16生,山东省阳信县人,汉族, 1998年滨州医学院本科毕业,1998-2004年于第四军医大学攻读硕、博士学位.主要从事乙型病毒性肝炎防治的研究.
项目负责人:陈红梅,710038, 陕西省西安市,中国人民解放军第四军医大学唐都医院感染科.
电话:029-87259845
收稿日期:2003-11-26 接受日期:2004-01-08
Construction and expression of eukaryoticvector bearing fusion geneof HBV PreS2S and Fc fragment
Hong-MeiChen, Xue-Fan Bai, Chang-Xing Huang, Gang-Yu Li, Sha Hong
Hong-Mei Chen, Xue-Fan Bai, Chang-Xing Huang, Gang-Yu Li, Sha Hong,Department of Infectious Diseases, Tangdu Hospital, Fourth MilitaryMedical University, Xi'an710038, Shaanxi Province, China
Correspondence to: Hong-Mei Chen, Department of InfectiousDiseases, Tangdu Hospital, Fourth Military Medical University, Xi'an710038, Shaanxi Province, China.
Received: 2003-11-26 Accepted:2004-01-08
AbstractAIM: To construct and express a recombinant eukaryotic expressionvector bearing fusion gene of HBV S2S and Fc fragment.
METHODS: The technique of splicing by overlapping extension and twice PCRwere used, and fusion gene fragment was obtained and cloned into pGEM-TEasy TA cloning vector to get suited enzyme sites. Recombinant eukaryoticexpression vector pcDNA3 S2S/Fc was constructed by double adhesiveterminal ligation. Then the recombinant vector was transferred into SP2/0cells by using Lipofectamine. ......
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