肝细胞与骨髓间质干细胞共同培养时的肝细胞功能
黄艳欣,杜雅菊,李宝杰,项目负责人,:,Effectonfunctionofrathepatocytesculturedwithbonemarrowcells,Correspondenceto:,Abstract,摘要,目的,方法,结果,结论,0,引言,1,材料和方法,1.1材料,1.2方法,统计学处理,2,结果,2.1肝细胞活
黄艳欣,杜雅菊, 李宝杰,哈尔滨医科大学附属第二医院消化内科 黑龙江省哈尔滨市 150086黄艳欣,女, 1977-11-14生,黑龙江省哈尔滨人,汉族. 2001年哈尔滨医科大学硕士生.
项目负责人:杜雅菊,150086, 黑龙江省哈尔滨市,哈尔滨医科大学附属第二医院消化内科.
电话:0451-86605143
收稿日期:2003-12-10 接受日期:2004-02-01
Effect on function of rat hepatocytes cultured with bone marrow cells
Yan-Xin Huang, Ya-Ju Du, Bao-Jie Li
Yan-Xin Huang, Ya-Ju Du, Bao-Jie Li, Department of Gastroenterology,The Second Hospital of Harbin Medical University, Harbin 150086,Heilongjiang Province, China
Correspondence to: Dr. Ya-Ju Du, Department of Gastroenterology,The Second Hospital of Harbin Medical University, Harbin 150086,Heilongjiang Province, China
Received: 2003-12-10 Accepted:2004-02-01
AbstractAIM: To study of the effect on the function of rat hepatocytescultured with bone marrow cells.
METHODS: Rat hepatocytes were isolated by the modified two-step methoddescribed by Seglen. The primary cultured hepatocytes and bone marrowcells were served as cocultured group, and single cultured hepatocytes ascontrol group. The yield and viability were assessed by trypan blueexclusion. The morphologic changes of cultured hepatocytes were observed.The concentrations of albumin and urea in the supernatant in differentcultural period were examined. ......
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