以食高岭土作为判定大鼠发生运动病指标的有效性
http://www.100md.com
2005年2月26日
以食高岭土作为判定大鼠发生运动病指标的有效性
付静宜 于立身* 刘丽 王烨 曹颖
(100036 北京，空军总医院空军临床分子生物学中心实验室；*空军航空医学研究所)
【摘要】 目的 改进用食高岭土作为判定大鼠发生诱发性运动病的方法。 方法 ①用122只雄性SD大鼠观察自然条件下食高岭土的特点；②将81只雄性SD大鼠分为用梯形旋转前庭刺激实验组和对照组做刺激前、后食高岭土情况的观察，根据大鼠在刺激前是否进食高岭土分别建立判定发生运动病的指标和计算大鼠运动病的发生率，同时与文献上常用的以进食高岭土为指标方法判定大鼠发生运动病情况进行比较。 结果 在自然饲养条件下未受前庭刺激的大鼠不只在适应期的前2 d进食高岭土，就是到第6天，仍有39.3%的大鼠进食高岭土，食否高岭土对饲料摄取量没有影响。用本研究提出的方法判定大鼠诱发性运动病发生率为22.5%，用其他文献中的方法判定的发生率或为0%，或为70%。 结论 本研究提出的方法能区分大鼠运动病易感性的不同，可排除假阳性或假阴性结果，从而可提高用进食高岭土作为指标判定大鼠是否发生运动病的有效性。
【关键词】 大鼠； 运动病； 高岭土
【中图分类号】 R852.33
The effectiveness of using kaolin intake as an index of motion sickness in rats FU Jing-yi*, YU Li-shen, LIU Li, et al. *General Hospital of Air Force, Beijing 100036, China
【Abstract】 Objective To revise the method of using kaolin intake as an index of motion sickness in rats. Methods The characteristic of kaolin intake were observed in 122 male SD rats under natural condition and in 81 male SD rats before and after rotational stimulation. In rats underwent rotational stimulation, the induced motion sickness were determined by the criteria established according to the characteristic of kaolin intake 3 days before and 3 days after rotational stimulation. Results Under natural condition, kaolin intake in rats is not limited in the first two days, on the 6th day of giving kaolin, 39.3% rats still took kaolin. Kaolin intake did not influence on the amount of feed intake. Using our evaluation criteria, the incidence of induced motion sickness in this group of rats was 22.5%, while using other criteria reported in references the incidence of induced motion sickness would be 0% or 70.0%. Conclusions Our evaluation criterion could distinguish rats susceptible to rotational stimulation and avoid the false results， so as to improve the effectiveness of using kaolin intake as an index of motion sickness in rats.
【Key words】 Rats; Motion sickness; Kaolin_, 百拇医药
付静宜 于立身* 刘丽 王烨 曹颖
(100036 北京，空军总医院空军临床分子生物学中心实验室；*空军航空医学研究所)
【摘要】 目的 改进用食高岭土作为判定大鼠发生诱发性运动病的方法。 方法 ①用122只雄性SD大鼠观察自然条件下食高岭土的特点；②将81只雄性SD大鼠分为用梯形旋转前庭刺激实验组和对照组做刺激前、后食高岭土情况的观察，根据大鼠在刺激前是否进食高岭土分别建立判定发生运动病的指标和计算大鼠运动病的发生率，同时与文献上常用的以进食高岭土为指标方法判定大鼠发生运动病情况进行比较。 结果 在自然饲养条件下未受前庭刺激的大鼠不只在适应期的前2 d进食高岭土，就是到第6天，仍有39.3%的大鼠进食高岭土，食否高岭土对饲料摄取量没有影响。用本研究提出的方法判定大鼠诱发性运动病发生率为22.5%，用其他文献中的方法判定的发生率或为0%，或为70%。 结论 本研究提出的方法能区分大鼠运动病易感性的不同，可排除假阳性或假阴性结果，从而可提高用进食高岭土作为指标判定大鼠是否发生运动病的有效性。
【关键词】 大鼠； 运动病； 高岭土
【中图分类号】 R852.33
The effectiveness of using kaolin intake as an index of motion sickness in rats FU Jing-yi*, YU Li-shen, LIU Li, et al. *General Hospital of Air Force, Beijing 100036, China
【Abstract】 Objective To revise the method of using kaolin intake as an index of motion sickness in rats. Methods The characteristic of kaolin intake were observed in 122 male SD rats under natural condition and in 81 male SD rats before and after rotational stimulation. In rats underwent rotational stimulation, the induced motion sickness were determined by the criteria established according to the characteristic of kaolin intake 3 days before and 3 days after rotational stimulation. Results Under natural condition, kaolin intake in rats is not limited in the first two days, on the 6th day of giving kaolin, 39.3% rats still took kaolin. Kaolin intake did not influence on the amount of feed intake. Using our evaluation criteria, the incidence of induced motion sickness in this group of rats was 22.5%, while using other criteria reported in references the incidence of induced motion sickness would be 0% or 70.0%. Conclusions Our evaluation criterion could distinguish rats susceptible to rotational stimulation and avoid the false results， so as to improve the effectiveness of using kaolin intake as an index of motion sickness in rats.
【Key words】 Rats; Motion sickness; Kaolin_, 百拇医药