血浆尿激酶型纤溶酶原激活物及其受体在急性胰腺炎中的测定及意义
陆新良, 浙江大学医学院附属二院消化科 浙江省杭州市 310009
诸景辉,余姚市第二人民医院消化科 浙江省余姚市 315410
卢兴国,浙江大学医学院附属二院血液科 浙江省杭州市 310009
姒健敏, 浙江大学医学院邵逸夫临床医学研究所 浙江省杭州市 310031
通讯作者:陆新良, 310009, 浙江省杭州市解放路88号, 浙江大学医学院附属二院消化科. luxl@zj.com
电话: 0571-87783715
, http://www.100md.com
收稿日期: 2004-09-13 接受日期: 2004-09-30
摘要目的:检测急性胰腺炎患者血浆尿激酶型纤溶酶原激活物(u-PA)及其受体(u-PAR)水平，并探讨了与胰腺Balthazar CT分级的关系.
方法:收集32例急性胰腺炎患者和32例正常对照组，采用ELISA法测定血浆u-PA和u-PAR，并对急性胰腺炎患者血浆u-PA和u-PAR与胰腺Balthazar CT分级进行Pearson相关分析.
结果:急性胰腺炎患者血浆u-PA和u-PAR水平明显高于正常对照组.血浆u-PA水平为1 290.66±484.28 ng/L，高于正常对照组1 034.63±267.55 ng/L(t = 2.618，p= 0.012);血浆u-PAR水平为1 240.00±442.84 ng/L，高于正常对照组870.00±265.72 ng/L(t = 4.053，p= 0.000).经Pearson相关分析，急性胰腺炎患者血u-PA与胰腺CT分级呈负相关(r= -0.433，p= 0.013)，血u-PAR与胰腺CT分级无关(r = 0.274，p= 0.129).
, 百拇医药
结论:血浆尿激酶型纤溶酶原激活物及其受体水平在急性胰腺炎患者中明显升高，其中血浆u-PA水平与胰腺Balthazar CT分级呈正相关.
陆新良, 诸景辉, 卢兴国, 姒健敏. 血浆尿激酶型纤溶酶原激活物及其受体在急性胰腺炎中的测定及意义. 世界华人消化杂志 2005;13(6):803-8051 Yue SQ, Yang YL, Zhou JS, Li KZ, Dou KF. Relationship between urokinase-type plasminogen activator receptor and
vascular endothelial growth factor expression and metastasis of gallbladder cancer. World J Gastroenterol
, http://www.100md.com 2004;10:2750-2752
2 Qin W, Zhu W, Wagner-Mann C, Sauter ER. Nipple aspirate fluid expression of urokinase type plasminogen activator,plasminogen activator inhibitor-1, and urokinase type plasminogen activator receptor predicts breast cancer diagnosis
and advanced disease. Ann Surg Oncol 2003;10:948-953
3 赵仲生, 姚根有, 茹国庆, 马杰, 徐文娟. 尿激酶型纤溶酶原激活物和基质金属蛋白酶9的mRNA表达与
胃癌浸润转移的关系. 中华普通外科杂志 2003;18:727-730
, 百拇医药
4 孙显路, 孙仁涛, 李玉军, 张顺, 吴力群. 尿激酶型纤溶酶原激活物及其受体表达与胰腺癌浸润
和转移的关系. 青岛大学医学院学报 2003;39:244-245
5 Wang M, Vogel I, Kalthoff H. Correlation between metastatic potential and variants from colorectal tumor cell line
HT-29. World J Gastroenterol 2003;9:2627-2631
6 徐根波, 卢兴国, 赵小英, 张晓红, 罗吕宏, 许晓华. 髓系白血病血浆尿激酶型纤溶酶原激活物及其受体
的检测与临床意义. 中华内科杂志 2003;42:199-200
, 百拇医药
7 杨雁灵, 窦科峰, 李开宗. 肝细胞肝癌UPAR和VEGF表达与浸润转移的关系. 世界华人消化杂志
2002;10:381-383
8 Friess H, Duarte R, Kleeff J, Fukuda A, Tang WH, Graber H, Schilling M, Zimmermann A, Korc M, Buchler MW.
The plasminogen activator/plasmin system is up-regulated after acute necrotizing pancreatitis in human beings.
Surgery 1998;124:79-86
9 宋水江, 胡俊斌, 王红祥, 闻树群, 丁美萍, 黄鉴政. 急性脑梗死患者血浆尿激酶型纤溶酶原激活物
, http://www.100md.com
及其受体的表达. 中华医学杂志 2003;83:1583-1585
10 卢兴国, 王彩花, 陈焰, 毛建山, 徐根波. 消化系疾病患者血浆尿激酶型纤溶酶原激活物及其受体检测.
中华血液学杂志 2002;23:495
11 宋川, 杨恬, 曾益军. 皮肤创伤修复中uPA/uPAR的表达及作用的研究. 第三军医大学学报
2001;23:1396-1398
12 Dobosz M, Mionskowska L, Hac S, Dobrowolski S, Dymecki D, Wajda Z. Heparin improves organ microcirculatory
, http://www.100md.com
disturbances in caerulein-induced acute pancreatitis in rats. World J Gastroenterol 2004;10:2553-2556
13 Wang ZF, Liu C, Lu Y, Dong R, Xu J, Yu L, Yao YM, Liu QG, Pan CE. Dexamethasone and dextran 40 treatment of 32
patients with severe acute pancreatitis. World J Gastroenterol 2004;10:1333-1336
14 Zhao YQ, Liu XH, Ito T, Qian JM. Protective effects of rhubarb on experimental severe acute pancreatitis.
, http://www.100md.com
World J Gastroenterol 2004;10:1005-1009
15 赵刚, 王春友, 熊炯炘, 陈立波. 重症急性胰腺炎并发腹腔出血的放射介入治疗.
世界华人消化杂志 2004;12:1241-1243
16 张震环, 李维勤, 黎介寿. 连续性血液净化在重症急性胰腺炎中的作用. 世界华人消化杂志
2003;11:2015-2018
17 Venkatesan T, Moulton JS, Ulrich CD, Martin SP. Prevalence and predictors of severity as defined by Atlanta criteria
, http://www.100md.com
among patients presenting with acute pancreatitis. Pancreas 2003;26:107-110
18 吴丽颖, 程礼, 蒋海飙(编译), 王兴鹏, 许国铭(审阅). 急性胰腺炎诊治指南(一). 胰腺病学 2002;2:48-50
19 方裕强, 吴丽颖, 程礼, 蒋海飙(编译),王兴鹏, 许国铭(审阅). 急性胰腺炎诊治指南(二). 胰腺病学
2002;2:103-109
20 Abu-Zidan FM, Bonham MJ, Windsor JA. Severeity of acute pancreatitis: a multivariate analysis of oxidative
, http://www.100md.com
stress markers and modified Gasgow criteria. Br J Surg 2000;87:1019-1023
21 Gullo L, Migliori M, Olah A, Farkas G, Levy P, Arvanitakis C, Lankisch P, Beger H. Acute pancreatitis in five European
countries: etiology and mortality. Pancreas 2003;24:223-227
22 Nagar AB, Gorelick FS. Acute pancreatitis. Curr Opin Gastroenterol 2004;20:439-443
23 Zhou ZG, Chen YD. Influencing factors of pancreatic microcirculatory impairment in acute panceatitis.
, 百拇医药
World J Gastroenterol 2002;8:406-412
24 Salomone T, Tosi P, Palareti G, Tomassetti P, Migliori M, Guariento A, Saieva C, Raiti C, Romboli M, Gullo L.
Coagulative disorders in human acute pancreatitis:role for the D-Dimer. Pancreas 2003;26:111-116
25 Zhou ZG, Chen YD, Sun W, Chen Z. Pancreatic microcirculatory impairment in experimental acute pancreatitis in rats.
, 百拇医药
World J Gastroenterol 2002;8:933-936
26 Bong JJ, Ammori BJ, McMahon MJ, Kumar A, Turney JH, Norfolk DR. Thrombotic microangiopathy in acute
pancreatitis. Pancreas 2002;25:107-109
27 Bentrem DJ, Joehl RJ. Pancreas:healing response in critical illness. Crit Care Med 2003;31(8 Suppl):S582-S589
28 Zhang Q, Ni Q, Cai D, Zhang Y, Zhang N, Hou L. Mechanisms of multiple organ damages in acute necrotizing
, http://www.100md.com
pancreatitis. Chin Med J 2001;114:738-742
29 Gurewich V. Fibrinolysis:an unfinished agenda. Blood Coagul Fibrinolysis 2000;11:401-408
30 Biousse V. The coagulation system. J Neuroophthalmol 2003;23:50-62
31 Cao DJ, Guo YL, Colman RW. Urokinase-type plasminogen activator receptor is involved in mediating the apoptotic
effect of cleaved high molecular weight kininogen in human endothelial cells. Circ Res 2004;94:1227-1234
, 百拇医药
32 Colman RW, Jameson BA, Lin Y, Johnson D, Mousa SA. Domain 5 of high molecular weight kininogen(kininostatin)d
own-regulates endothelial cell proliferation and migration and inhibits angiogenesis. Blood 2000;95:543-550
33 Ilback NG, Benyamin G, Lindh U, Fohlman J, Friman G. Trace element changes in the pancreas during viral infection
in mice. Pancreas 2003;26:190-196
编辑 张海宁, 百拇医药( 陆新良,诸景辉, 卢兴国,姒健敏)
诸景辉,余姚市第二人民医院消化科 浙江省余姚市 315410
卢兴国,浙江大学医学院附属二院血液科 浙江省杭州市 310009
姒健敏, 浙江大学医学院邵逸夫临床医学研究所 浙江省杭州市 310031
通讯作者:陆新良, 310009, 浙江省杭州市解放路88号, 浙江大学医学院附属二院消化科. luxl@zj.com
电话: 0571-87783715
, http://www.100md.com
收稿日期: 2004-09-13 接受日期: 2004-09-30
摘要目的:检测急性胰腺炎患者血浆尿激酶型纤溶酶原激活物(u-PA)及其受体(u-PAR)水平，并探讨了与胰腺Balthazar CT分级的关系.
方法:收集32例急性胰腺炎患者和32例正常对照组，采用ELISA法测定血浆u-PA和u-PAR，并对急性胰腺炎患者血浆u-PA和u-PAR与胰腺Balthazar CT分级进行Pearson相关分析.
结果:急性胰腺炎患者血浆u-PA和u-PAR水平明显高于正常对照组.血浆u-PA水平为1 290.66±484.28 ng/L，高于正常对照组1 034.63±267.55 ng/L(t = 2.618，p= 0.012);血浆u-PAR水平为1 240.00±442.84 ng/L，高于正常对照组870.00±265.72 ng/L(t = 4.053，p= 0.000).经Pearson相关分析，急性胰腺炎患者血u-PA与胰腺CT分级呈负相关(r= -0.433，p= 0.013)，血u-PAR与胰腺CT分级无关(r = 0.274，p= 0.129).
, 百拇医药
结论:血浆尿激酶型纤溶酶原激活物及其受体水平在急性胰腺炎患者中明显升高，其中血浆u-PA水平与胰腺Balthazar CT分级呈正相关.
陆新良, 诸景辉, 卢兴国, 姒健敏. 血浆尿激酶型纤溶酶原激活物及其受体在急性胰腺炎中的测定及意义. 世界华人消化杂志 2005;13(6):803-8051 Yue SQ, Yang YL, Zhou JS, Li KZ, Dou KF. Relationship between urokinase-type plasminogen activator receptor and
vascular endothelial growth factor expression and metastasis of gallbladder cancer. World J Gastroenterol
, http://www.100md.com 2004;10:2750-2752
2 Qin W, Zhu W, Wagner-Mann C, Sauter ER. Nipple aspirate fluid expression of urokinase type plasminogen activator,plasminogen activator inhibitor-1, and urokinase type plasminogen activator receptor predicts breast cancer diagnosis
and advanced disease. Ann Surg Oncol 2003;10:948-953
3 赵仲生, 姚根有, 茹国庆, 马杰, 徐文娟. 尿激酶型纤溶酶原激活物和基质金属蛋白酶9的mRNA表达与
胃癌浸润转移的关系. 中华普通外科杂志 2003;18:727-730
, 百拇医药
4 孙显路, 孙仁涛, 李玉军, 张顺, 吴力群. 尿激酶型纤溶酶原激活物及其受体表达与胰腺癌浸润
和转移的关系. 青岛大学医学院学报 2003;39:244-245
5 Wang M, Vogel I, Kalthoff H. Correlation between metastatic potential and variants from colorectal tumor cell line
HT-29. World J Gastroenterol 2003;9:2627-2631
6 徐根波, 卢兴国, 赵小英, 张晓红, 罗吕宏, 许晓华. 髓系白血病血浆尿激酶型纤溶酶原激活物及其受体
的检测与临床意义. 中华内科杂志 2003;42:199-200
, 百拇医药
7 杨雁灵, 窦科峰, 李开宗. 肝细胞肝癌UPAR和VEGF表达与浸润转移的关系. 世界华人消化杂志
2002;10:381-383
8 Friess H, Duarte R, Kleeff J, Fukuda A, Tang WH, Graber H, Schilling M, Zimmermann A, Korc M, Buchler MW.
The plasminogen activator/plasmin system is up-regulated after acute necrotizing pancreatitis in human beings.
Surgery 1998;124:79-86
9 宋水江, 胡俊斌, 王红祥, 闻树群, 丁美萍, 黄鉴政. 急性脑梗死患者血浆尿激酶型纤溶酶原激活物
, http://www.100md.com
及其受体的表达. 中华医学杂志 2003;83:1583-1585
10 卢兴国, 王彩花, 陈焰, 毛建山, 徐根波. 消化系疾病患者血浆尿激酶型纤溶酶原激活物及其受体检测.
中华血液学杂志 2002;23:495
11 宋川, 杨恬, 曾益军. 皮肤创伤修复中uPA/uPAR的表达及作用的研究. 第三军医大学学报
2001;23:1396-1398
12 Dobosz M, Mionskowska L, Hac S, Dobrowolski S, Dymecki D, Wajda Z. Heparin improves organ microcirculatory
, http://www.100md.com
disturbances in caerulein-induced acute pancreatitis in rats. World J Gastroenterol 2004;10:2553-2556
13 Wang ZF, Liu C, Lu Y, Dong R, Xu J, Yu L, Yao YM, Liu QG, Pan CE. Dexamethasone and dextran 40 treatment of 32
patients with severe acute pancreatitis. World J Gastroenterol 2004;10:1333-1336
14 Zhao YQ, Liu XH, Ito T, Qian JM. Protective effects of rhubarb on experimental severe acute pancreatitis.
, http://www.100md.com
World J Gastroenterol 2004;10:1005-1009
15 赵刚, 王春友, 熊炯炘, 陈立波. 重症急性胰腺炎并发腹腔出血的放射介入治疗.
世界华人消化杂志 2004;12:1241-1243
16 张震环, 李维勤, 黎介寿. 连续性血液净化在重症急性胰腺炎中的作用. 世界华人消化杂志
2003;11:2015-2018
17 Venkatesan T, Moulton JS, Ulrich CD, Martin SP. Prevalence and predictors of severity as defined by Atlanta criteria
, http://www.100md.com
among patients presenting with acute pancreatitis. Pancreas 2003;26:107-110
18 吴丽颖, 程礼, 蒋海飙(编译), 王兴鹏, 许国铭(审阅). 急性胰腺炎诊治指南(一). 胰腺病学 2002;2:48-50
19 方裕强, 吴丽颖, 程礼, 蒋海飙(编译),王兴鹏, 许国铭(审阅). 急性胰腺炎诊治指南(二). 胰腺病学
2002;2:103-109
20 Abu-Zidan FM, Bonham MJ, Windsor JA. Severeity of acute pancreatitis: a multivariate analysis of oxidative
, http://www.100md.com
stress markers and modified Gasgow criteria. Br J Surg 2000;87:1019-1023
21 Gullo L, Migliori M, Olah A, Farkas G, Levy P, Arvanitakis C, Lankisch P, Beger H. Acute pancreatitis in five European
countries: etiology and mortality. Pancreas 2003;24:223-227
22 Nagar AB, Gorelick FS. Acute pancreatitis. Curr Opin Gastroenterol 2004;20:439-443
23 Zhou ZG, Chen YD. Influencing factors of pancreatic microcirculatory impairment in acute panceatitis.
, 百拇医药
World J Gastroenterol 2002;8:406-412
24 Salomone T, Tosi P, Palareti G, Tomassetti P, Migliori M, Guariento A, Saieva C, Raiti C, Romboli M, Gullo L.
Coagulative disorders in human acute pancreatitis:role for the D-Dimer. Pancreas 2003;26:111-116
25 Zhou ZG, Chen YD, Sun W, Chen Z. Pancreatic microcirculatory impairment in experimental acute pancreatitis in rats.
, 百拇医药
World J Gastroenterol 2002;8:933-936
26 Bong JJ, Ammori BJ, McMahon MJ, Kumar A, Turney JH, Norfolk DR. Thrombotic microangiopathy in acute
pancreatitis. Pancreas 2002;25:107-109
27 Bentrem DJ, Joehl RJ. Pancreas:healing response in critical illness. Crit Care Med 2003;31(8 Suppl):S582-S589
28 Zhang Q, Ni Q, Cai D, Zhang Y, Zhang N, Hou L. Mechanisms of multiple organ damages in acute necrotizing
, http://www.100md.com
pancreatitis. Chin Med J 2001;114:738-742
29 Gurewich V. Fibrinolysis:an unfinished agenda. Blood Coagul Fibrinolysis 2000;11:401-408
30 Biousse V. The coagulation system. J Neuroophthalmol 2003;23:50-62
31 Cao DJ, Guo YL, Colman RW. Urokinase-type plasminogen activator receptor is involved in mediating the apoptotic
effect of cleaved high molecular weight kininogen in human endothelial cells. Circ Res 2004;94:1227-1234
, 百拇医药
32 Colman RW, Jameson BA, Lin Y, Johnson D, Mousa SA. Domain 5 of high molecular weight kininogen(kininostatin)d
own-regulates endothelial cell proliferation and migration and inhibits angiogenesis. Blood 2000;95:543-550
33 Ilback NG, Benyamin G, Lindh U, Fohlman J, Friman G. Trace element changes in the pancreas during viral infection
in mice. Pancreas 2003;26:190-196
编辑 张海宁, 百拇医药( 陆新良,诸景辉, 卢兴国,姒健敏)
